Short-Term course in Human Embryonic Stem Cell Culture Techniques

描述（由申请人提供）：“人类胚胎干细胞工具箱（HEST）”研讨会的目标是提供详细的“动手”培训，以提高参与者未来在人类胚胎干细胞（hESC）实验中获得更统一和信息丰富的结果的成功。培养hESC不能直接从方案和/或教科书中教导或理解。技术或“工具”正在发展，需要与受过培训的个人进行一对一的演示。HEST研讨会有经验丰富的教师在发育生物学，基因表达和细胞遗传学的培训。参与者将在为期5天的研讨会（4：1的参与者与讲师比例）中学习hESC细胞自我更新的复杂细节，“如何”和理论。目标是：1.）教育和培训研究人员使用NIH批准的细胞系进行人类胚胎细胞方法。(BG01，BG 02和WA 09）。2.）利用经过测试的关键材料启动参与者研究。3.）第三章在研讨会参与者与HEST教师和协调员之间建立持续的渠道，以进行继续教育，培训和支持。4.）持续评估和改进HEST教育和培训。我们即将进行我们的第四次HEST研讨会（2005年10月），在我们最初的资助结束时完成了5次，有80多名参与者。之前的参与者已经获得了NIH的资助，并在他们的机构中启动了hESC核心设施。为此提议举办6次HEST讲习班（5天）。所有3年（4个讲习班）的网站是格鲁吉亚大学。从第二年和第三年开始，将在白石研究所举办两个讲习班。两个地点将教授相同的首席教员和课程。这一交流提供了一个机会，让讲师有不同的培训和专业知识（提供信件），并吸引当地参与者。各机构之间的相互交流将在第二年和第三年加强已经非常成功的HEST讲习班的内容。NIH资助的与使用hESC相关的研究正在增加，拟议的HEST研讨会将满足这一需求。HEST研讨会将通过培训生物医学研究人员“如何”培养和维持hESC用于他们的研究来推进公共卫生方面的相关研究。

DESCRIPTION (provided by applicant): The goal of the "Human Embryonic Stem cell toolbox (HEST) workshop is to provide detailed "hands on" training to enhance the participants' future success in obtaining more uniform and informative results in their human embryonic stem cell (hESC) experiments. Propagating hESC cannot be taught or understood directly from protocols and or textbooks. The techniques or "tools" are evolving and requires one on one demonstration with trained individuals. The HEST workshop has experienced instructors trained in developmental biology, gene expression and cytogenetics. The participants will learn the intricate details, the "how too" and theory of hESC cell self renewal during a 5 day workshop (4:1 participant to instructor ratio). The aims are: 1.) To educate and train researchers in human embryonic cell methods using the NIH approved cell lines. (BG01, BG02 and WA 09). 2.) To jumpstart participant research utilizing hESCs with tested critical materials. 3.) To establish an ongoing conduit between workshop participants and HEST faculty and coordinator for continued education, training and support. 4.) To obtain ongoing evaluation and improvement of HEST education and training. We are about to conduct our 4th HEST workshop (Oct 2005), with 5 completed by the end of our initial grant with over 80 participants. Prior participants have obtained NIH funding and started hESC core facilities in their institutions. For this proposal, 6 HEST workshops (five days) are proposed. The site for all 3 yrs (4 workshops) is the University of Georgia. Starting in yr 2 and 3, 2 workshops will be conducted at the Whitehead Institute. The same head instructors and curriculum will be taught at both sites. This exchange provides an opportunity to have lecturers with different training and expertise (letters provided) and to draw in local participants. Cross fertilization between institutions will enhance the content of an already highly successful HEST workshop during the second and third years. NIH funded research relating to using hESC is increasing and the proposed HEST workshop will meet this demand. The HEST workshop will advance relevant research in public health by training biomedical researchers the "how to" grow and maintain hESC for use in their research.