Core C- Physiology

综合生理学核心C：项目总结 综合生理学核心是一种多服务资源，为中心研究者及其 实验室建立和研究小鼠（包括转基因和基因敲除）所需的工具和建议 和胃肠道疾病的人（即3D类器官培养物）模型。自本中心成立以来， 这一核心已经扩大了研究基地的使用范围，并成为一个具有高度成本效益和效率的 组织学、基因分型和生理学服务的资源。该核心还为研究基地提供了 利用人类肠道干细胞技术进行研究的培训和资源。使用人 肠干细胞衍生的类肠/类结肠已经迅速发展，并正在成为治疗结肠炎的首要模型。 研究人类胃肠道生理学和病理生理学。在最初供资期间，46个国家使用了核心方案， 核心中心研究者（>70%利用多种服务），发表了65篇论文， 在11个获资助的P/F项目中，有7个项目使用了该系统。所提供的服务包括：1）就建立和 维持小鼠群体，包括如何在统一的背景下繁殖; 2）基因分型，包括 开发和优化引物; 3）组织学服务，包括组织固定和处理， 包埋、切片（包括冷冻切片）、组织病理学染色（例如H&E、PAS、天狼星红），以及 组织存档。本研究基地研究的小鼠模型胃肠道器官H&E切片组织库 提供给其他核心成员用于初步研究; 4）由一个 用于表征小鼠GI疾病模型的专家病理学家; 5）代谢笼可用于 代谢平衡研究，包括血液、尿液、粪便分析; 6）Ussing室/电压钳技术 用于测量活性离子转运和紧密连接渗透性和选择性渗透性;7）用于测量活性离子转运和紧密连接渗透性和选择性渗透性的多重ELISA。 从小体积中同时测量约100种分析物（例如细胞因子、趋化因子、生长因子） （<100μl）血清、组织或培养基样本;以及8）建立、维持和 使用小鼠和人的肠样组织以及提供长期- 文化术语

Integrated Physiology Core C: Project Summary The Integrated Physiology Core is a multi-service resource that provides Center Investigators and their laboratories the tools and advice needed to establish and study mouse (including transgenic and knockout) and human (i.e. 3D organoid cultures) models of gastrointestinal disease. Since the inception of our Center, this Core has expanded its use by the Research Base and become a highly cost effective and efficient resource for histology, genotyping, and physiology services. This Core also provides the Research Base with the training and resources to utilize human intestinal stem cell technology for their studies. The use of human intestinal stem cell derived enteroids/colonoids has rapidly evolved and is becoming a premier model for the study of human GI physiology and pathophysiology. During the initial funding period, the Core was used by 46 Core Center investigators (>70% utilized multiple services), resulting in 65 published manuscripts, and has been used by 7 out of 11 funded P/F projects. The services offered include 1) Advice on establishing and maintaining mouse colonies, including how to breed onto uniform backgrounds; 2) Genotyping, which includes developing and optimizing primers; 3) Histological services, which include tissue fixation and processing, embedding, sectioning (including cryosectioning), histopathological staining (e.g. H&E, PAS, Sirius Red), and tissue archiving. A tissue bank of H&E slides of GI organs of the mouse models studied by our Research Base are made available for other Core members to use for preliminary studies; 4) Histopathology consultation by an expert pathologist for characterization of mouse GI disease models; 5) Metabolic cages are available for metabolic balance studies, including blood, urine, stool analysis; 6) Ussing chamber/voltage clamp technology for measuring active ion transport and tight junction permeability and permselectivity;7) Multiplex ELISA for simultaneous measurement of ~100 analytes (e.g. cytokines, chemokines, growth factors) from a small volume (<100μl) sample of serum, tissue, or culture media; as well as 8) Training in establishing, maintaining, and using mouse and human enteroids as well as provision of growth factor conditioned media necessary for long- term culture.