Core C- Physiology

综合生理学核心C：项目总结 综合生理学核心是一个多服务资源，为中心研究人员和他们的 实验室建立和研究小鼠所需的工具和建议(包括转基因和基因敲除) 以及胃肠道疾病的人体(即3D器官培养)模型。本中心自成立以来， 这一核心扩大了研究基地的使用范围，成为一种高性价比和高效率的 组织学、基因分型和生理学服务的资源。该核心还为研究基地提供 利用人类肠道干细胞技术进行研究的培训和资源。人类的使用 肠干细胞来源的肠样/结肠样体进化迅速，并正在成为 人体胃肠道生理学和病理生理学的研究。在最初的筹资期间，有46人使用了核心 核心中心调查人员(70%利用多种服务)，发表了65篇手稿，并 已被11个受资助的P/F项目中的7个使用。所提供的服务包括1)关于建立和 维持小鼠群体，包括如何在统一的背景下繁殖；2)基因分型，包括 开发和优化引物；3)组织学服务，包括组织固定和加工， 包埋、切片(包括冰冻切片)、组织病理学染色(如H&E、PAS、天狼星红)； 组织归档。本研究基地建立的小鼠胃肠道组织H&E切片组织库 供其他核心成员用于初步研究；4)组织病理学会诊 描述小鼠胃肠道疾病模型的专家病理学家；5)代谢笼可用于 代谢平衡研究，包括血液、尿液、大便分析；6)使用电压室/电压钳技术 用于测量活性离子转运和紧密连接渗透性和渗透选择性；7)多重酶联免疫吸附试验 从小容量同时测量~100种分析物(如细胞因子、趋化因子、生长因子) (&lt；100μL)血清、组织或培养液样本；以及8)建立、维护和 使用小鼠和人的肠样体，以及提供长期必需的生长因子条件培养液 术语文化。

Integrated Physiology Core C: Project Summary The Integrated Physiology Core is a multi-service resource that provides Center Investigators and their laboratories the tools and advice needed to establish and study mouse (including transgenic and knockout) and human (i.e. 3D organoid cultures) models of gastrointestinal disease. Since the inception of our Center, this Core has expanded its use by the Research Base and become a highly cost effective and efficient resource for histology, genotyping, and physiology services. This Core also provides the Research Base with the training and resources to utilize human intestinal stem cell technology for their studies. The use of human intestinal stem cell derived enteroids/colonoids has rapidly evolved and is becoming a premier model for the study of human GI physiology and pathophysiology. During the initial funding period, the Core was used by 46 Core Center investigators (>70% utilized multiple services), resulting in 65 published manuscripts, and has been used by 7 out of 11 funded P/F projects. The services offered include 1) Advice on establishing and maintaining mouse colonies, including how to breed onto uniform backgrounds; 2) Genotyping, which includes developing and optimizing primers; 3) Histological services, which include tissue fixation and processing, embedding, sectioning (including cryosectioning), histopathological staining (e.g. H&E, PAS, Sirius Red), and tissue archiving. A tissue bank of H&E slides of GI organs of the mouse models studied by our Research Base are made available for other Core members to use for preliminary studies; 4) Histopathology consultation by an expert pathologist for characterization of mouse GI disease models; 5) Metabolic cages are available for metabolic balance studies, including blood, urine, stool analysis; 6) Ussing chamber/voltage clamp technology for measuring active ion transport and tight junction permeability and permselectivity;7) Multiplex ELISA for simultaneous measurement of ~100 analytes (e.g. cytokines, chemokines, growth factors) from a small volume (<100μl) sample of serum, tissue, or culture media; as well as 8) Training in establishing, maintaining, and using mouse and human enteroids as well as provision of growth factor conditioned media necessary for long- term culture.