Core A Islet Production

摘要/概要 核心A：胰岛生产核心。胰岛功能和完整性代表葡萄糖稳态的关键节点。 胰岛生产核心支持DRC研究人员的研究，他们分析了 在从小鼠和人的胰腺分离的胰岛中进行遗传或药物干预。大学旧金山 刚果民主共和国也有一个非常强大的胰岛免疫和移植计划。孤立的胰岛对于 研究方向是对抗胰岛自身免疫和减少移植排斥。 目的：刚果民主共和国胰岛生产核心巩固，增强和传播专业知识和资源， 向目前正在研究或计划研究胰岛细胞生物学的DRC研究者提供胰岛制备， 胰岛功能和/或移植。Core提供以下功能： 1.胰岛纯化。用于胰岛生物学的人和小鼠胰岛的加工和制备， 自身免疫和移植研究。 2.胰岛功能分析。胰岛素生产和其他的真实的实时生物和生化分析 人和小鼠胰岛的功能方面，以表征不同条件下的胰岛活性， 评估用于移植研究的胰岛制备质量。 对刚果民主共和国社区的好处：刚果民主共和国调查人员不需要投入时间和资源来建立这个高度 实验室内的专业能力。不同DRC采用的胰岛制备方法一致 调查人员还可以进行跨研究比较，以在研究之间产生意料之外的联系。35 目前，该核心项目正在为不同的项目提供资金，每年的直接费用共计6，580，009美元。 仅在过去的2年中，小鼠胰岛共制备了807次（每次制备1至20只小鼠），用于 12个DRC研究实验室。另外45个小鼠胰岛制备用于3个其他研究者 因为他们考虑他们的研究是否可以应用于糖尿病研究。UCSF也有一个领先的 临床胰岛移植计划。为这些人类胰岛移植开发的设施已经 利用DRC使人类胰岛可用于研究。在过去的一年里， 制备胰岛供6名DRC研究者研究。根据设施的容量和可用性 人类研究级胰腺，我们建议提供这种独特的人类胰岛功能， UCSF以外的研究人员（见区域核心）。 技术开发：人类和模式生物的独特能力促进了翻译 UCSF和整个地区的核心用户的研究。刚果民主共和国也有强大的计划， 通过刚果民主共和国的赠款，已成功地开发了胰岛制备监测的改进， 和功能这使得核心的活动不仅有利于转化和基础研究， 还有临床试验和参与其中的病人。

Abstract/Summary Core A: Islet Production Core. Islet function and integrity represent a critical node in glucose homeostasis. The Islet Production Core supports research by DRC investigators who analyze the functional responses to genetic or pharmacologic interventions in islets isolated from pancreases of mice and humans. The UCSF DRC also has a very strong islet immunology and transplantation program. Isolated islets are essential for studies oriented towards counteracting islet autoimmunity and minimizing transplant rejection. Purpose: The DRC Islet Production Core consolidates, enhances and disseminates expertise and resources in islet preparation to DRC investigators who currently study or have plans to study pancreatic islet cell biology, islet function and/or transplantation. The Core provides the following capabilities: 1. Islet Purification. Processing and preparation of human and mouse islets for use in islet biology, autoimmunity and transplantation studies. 2. Islet Functional Analysis. Real time biological and biochemical analysis of insulin production and other functional aspects of human and mouse islets to characterize islet activity under different conditions and to assess islet preparation quality for transplantation studies. Benefits to DRC Community: DRC investigators need not commit time and resources to establishing this highly specialized capability within their laboratory. The uniform islet preparation methods used by different DRC investigators also can enable cross-study comparisons to generate unanticipated linkages between studies. 35 different projects totaling $6,580,009 annual direct costs currently are benefiting from this Core. In the past 2 years alone, mouse islets were prepared a total of 807 times (from 1 to 20 mice per prep) for 12 DRC research laboratories. A further 45 mouse islet preparations were conducted for 3 other investigators as they considered whether their studies had applications to diabetes research. UCSF also has a leading clinical islet transplantation program. The facilities developed for those human islet transplants have been leveraged by the DRC for making human islets available for research studies. In the last year, human research islets were prepared for studies by six DRC investigators. With the capacity of the facilities and the availability of human research-grade pancreases, we propose to provide this unique human islets capability to investigators beyond UCSF (see Regional Core). Technology Development: Unique capabilities in human and model organisms facilitate translational research by Core users at UCSF and throughout the region. The DRC also has robust programs, funded through the DRC grant, that have successfully developed improvements in the monitoring of islet preparation and function. This enables the activities of the Core to benefit not only translational and basic research, but also clinical trials and the patients participating in them.