Oklahoma Nathan Shock Center of Excellence in Basic Biology of Aging

俄克拉荷马州内森休克衰老基础生物学卓越中心

• 批准号: 9110089
• 负责人: ARLAN G. RICHARDSON
• 金额: $ 74.85万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9110089
• 关键词: Aging; Animal Model; Animals; Arts; Bioinformatics; Biological Assay; Biology; Biology of Aging; Caenorhabditis elegans Proteins; Cells; Chronic Disease; Cities; Communities; Country; Couples; DNA Methylation; Data; Deletion Mutation; Development; Discipline; Disease; Drosophila genus; Education; Faculty; Foundations; Funding; Funding Agency; Genes; Geroscience; Goals; Grant; Health; Health Sciences; Human; Institutes; Institution; Interest Group; Leadership; Life; Mass Spectrum Analysis; Measurement; Measures; Medical Research; Medical center; Mentors; Methods; Mitochondria; Molecular; Oklahoma; Onset of illness; Organism; Oxidation-Reduction; Oxidative Stress; Pathway interactions; Peptides; Proteins; Research; Research Personnel; Resources; Risk Factors; Role; Running; Sampling; Series; Services; Shock; Speed; Structure; Technology; Time; Tissues; United States National Institutes of Health; Universities; Variant; Yeasts; age related; career; catalyst; design; disorder prevention; experience; genome sequencing; genome-wide; innovation; instrumentation; interest; mass spectrometer; mitochondrial genome; next generation sequencing; non-genetic; novel; oxidative damage; programs; research and development; tool

DESCRIPTION (provided by applicant): The Oklahoma Nathan Shock Center will focus on the newly-developing field of Geroscience and on providing investigators across the nation with unique and innovative assays that are accessible to the entire research community studying aging and Geroscience. The 5 Cores in the Oklahoma Nathan Shock Center are: Program Enhancement Core: In addition, to overseeing all the activities of the Shock Center, the Program Enhancement Core will administer two novel programs. The development of the Oklahoma Geroscience Consortium will bring together investigators interested in aging and Geroscience from the five major research institutions in Oklahoma. In addition, through a Discovery Bioinformatics Component, the Core will provide investigators in aging with unique bioinformatics tools and methods to discover new connections between their data and age-related phenomenon. Research Development Core: This Core is an innovative departure from the traditional structure of similar Cores in Nathan Shock Centers. The Research Development Core of the Oklahoma Nathan Shock Center is designed to provide unprecedented service to the scientific community at large, not just investigators at the host institution. Multiplexing Protein Quantification Core: This Core will provide investigators with a novel mass spectrometry method for measuring the abundance of proteins from any tissue/cell from any organism that has its genome sequenced. The Core can quantify panels of 25 to 30 proteins in a single run, thereby allowing investigators to interrogate pathways of importance in aging and Geroscience. By developing panels of proteins for C elegans, Drosophila, and yeast, as well as exceptionally long-lived animals, this Core will provide investigators, for the first-time, a method for measurin protein abundance in these animal models, which are important to aging. Targeted DNA Methylation & Mitochondrial Heteroplasmy Core: Using novel next-generation sequencing with unique primer sets, which are currently available only in this Core, it will be possible for investigators to study DNA methylation either genome-wide or in specific genes. In addition, the Core has developed methods that will allow investigators to conduct a comprehensive analysis of mitochondrial genome heteroplasmy (variants/mutations and deletions) and copy number from very small samples in a high throughput manner. Integrative Redox Biology Core: This Core will provide investigators with a comprehensive state-of-the-art assessment of the oxidative stress status of a cell, e.g., measures of oxidative damage and redox couples, which require major instrumentation and expertise to obtain accurate and reproducible data. In the past, the Leaders of this Core have experienced a high demand for these assays from investigators across the country.

描述（由申请人提供）：俄克拉荷马州内森休克中心将重点关注新发展的老年科学领域，并为全国各地的研究人员提供独特和创新的检测方法，整个研究衰老和老年科学的研究界都可以使用这些检测方法。俄克拉荷马州内森休克中心的 5 个核心是： 计划增强核心：此外，为了监督休克中心的所有活动，计划增强核心将管理两个新颖的计划。俄克拉荷马州老年科学联盟的发展将汇集俄克拉荷马州五个主要研究机构对衰老和老年科学感兴趣的研究人员。此外，通过发现生物信息学组件，该核心将为衰老研究人员提供独特的生物信息学工具和方法，以发现其数据与年龄相关现象之间的新联系。研究开发核心：该核心与内森休克中心类似核心的传统结构不同，具有创新性。俄克拉荷马州内森休克中心的研究开发核心旨在为整个科学界（而不仅仅是主办机构的研究人员）提供前所未有的服务。多重蛋白质定量核心：该核心将为研究人员提供一种新颖的质谱方法，用于测量基因组已测序的任何生物体的任何组织/细胞的蛋白质丰度。 Core 可以在一次运行中量化 25 至 30 种蛋白质的组合，从而使研究人员能够探究衰老和老年科学中的重要途径。通过开发线虫、果蝇和酵母以及特别长寿的动物的蛋白质组，该核心将首次为研究人员提供一种测量这些动物模型中蛋白质丰度的方法，这对衰老很重要。靶向 DNA 甲基化和线粒体异质性核心：使用具有独特引物组的新型下一代测序（目前仅在该核心中可用），研究人员将可以研究全基因组或特定基因中的 DNA 甲基化。此外，核心还开发了一些方法，使研究人员能够以高通量方式对非常小的样本中的线粒体基因组异质性（变异/突变和缺失）和拷贝数进行全面分析。综合氧化还原生物学核心：该核心将为研究人员提供对细胞氧化应激状态的全面、最先进的评估，例如氧化损伤和氧化还原对的测量，这需要主要仪器和专业知识才能获得准确且可重复的数据。过去，该核心的领导者经历了全国各地研究人员对这些检测方法的高需求。