ADMINISTRATIVE SUPPLEMENT TO GRANT R01-AG057424, Short-term Measurements of Physical Resilience as a Predictor of Healthspan in Mice.

这一行政补充申请代表着一个新的机会，它已经出现在 NIA资助的老年病理学评分平台(GGP)的最新进展 研究网络。这种对老鼠病理的新评估是在亲代之后才完全发展起来的 格兰特被提交了。要求提供资金以支持兽医布雷希尔斯博士的工资 病理学家将使用最近开发的GGP来评估将被 在AIMS 2和AIMS 3中测试其对压力的弹性。这里要求的补充品将允许我们使用 新开发的GGP在我们将用来测量的相同小鼠中测量全球病理 对压力的恢复能力。在对小鼠进行弹性测试后，它们将被终止，全球 每只动物的病理状态将由GGP进行评估。因为病理性的发生 损伤是衰老的标志，小鼠的整体病理状态给了我们一个健康跨度的衡量标准。 因此，我们将能够确定我们的复原力测量对小鼠健康寿命的预测效果如何 我们在学习。此外，这些实验将首次使用GGP来评估 饮食限制、阿卡波糖和17-α-雌二醇等干预对全球病理学的影响。

This administrative supplement application represents a new opportunity that has arisen from the recent development of the Geropathology Grading Platform (GGP) by the NIA funded Geropathology Research Network. This novel assessment of mouse pathology was only fully developed after the parent grant was submitted. Funds are requested to support the salary of Dr. Breshears, a veterinary pathologist, who will use the recently developed GGP to assess health status of the animals that will be tested in Aims 2 and 3 for their resilience to stress. The supplement requested here will allow us to use the newly developed GGP to measure global pathology in the same mice that we will use to measure resilience to stress. After the mice have been tested for resilience, they will be terminated, and the global pathological status of each animal will be assessed by the GGP. Because the occurrence of pathological lesions is a hallmark of aging, the global pathological status of the mice gives us a measure of healthspan. Thus, we will be able to determine how well our measures of resilience predict the healthspan of the mice we are studying. In addition, these experiments will be the first to use the GGP to assess the effect of interventions such as dietary restriction, acarbose, and 17-α-estradiol on global pathology.