ShEEP Request for Cell Sorter

摘要 我们正在申请资金购买Miltyeni Tyto细胞分选系统。该工具允许调查人员 基于来自转基因的荧光标记或来自细胞表面标记的荧光标记以不同于 常规细胞分选系统。该文书内的一些技术进步增加了 通量、易用性、无菌性和细胞活力。目前，无论是在俄克拉荷马州的研究机构 VA医学中心（OKC VAMC）或附属的俄克拉荷马州大学健康科学中心有能力 以进行这种类型的低压细胞分选样品收集。通过细胞培养和动物模型， 研究研究收集特定细胞群用于随后培养、分子或 从复杂的细胞混合物进行生化分析是至关重要的。能够收集特定的细胞类型， 了解健康和疾病中的细胞功能以及多个细胞之间的不同变化的优势 细胞类型以细胞非自主的方式工作以引起组织功能障碍和疾病。传统上，Cell 分选技术需要专门的鞘液用于悬浮细胞、高压分选和复杂的分离。 仪器.一种新的细胞分选方法使用微芯片盒来执行细胞分选的所有方面 而不使用高压或专门的鞘液。这就形成了一个完全无菌的一次性 细胞分选的环境，也更温和地处理细胞，增加它们的活力， 培养或引起较少的分子和生物化学变化用于随后的分析。 选择Miltyeni Tyto仪器是基于仪器的多功能性，以满足各种需求 俄克拉荷马州退伍军人管理委员会的调查员该系统能够为各种应用分选细胞，包括 神经科学，免疫学，胃肠道和癌症研究满足了我们不同的研究人员的需求。 研究领域。所有的研究者都可以利用这种细胞分选能力进行后续的传代培养 细胞，或收集细胞用于生物化学和分子分析。易于使用，减少维护， 与传统的细胞分选机相比，完全无菌是各种细胞分选机使用的核心仪器的优点。 的群体。仪器选择的另一个核心是三个集成激光器， 细胞参数排序。 对OKC VAMC所有VA研究人员的调查显示，各种各样的研究人员将使用该系统。这 仪器将由五个主要用户和两个次要用户使用，他们有五个VA优异赠款金和两个 高级职业科学家奖以及一些未来的用户。

Abstract We are requesting funds to purchase a Miltyeni Tyto cell sorting system. This instrument allows investigators to sort cells based on fluorescent markers from transgenes or from cell surface markers in a manner unlike conventional cell sorting systems. A number of technological advancements within this instrument increase the throughput, ease of use, sterility, and cell viability. Currently, neither the research facility at the Oklahoma City VA Medical Center (OKC VAMC) or the affiliated University of Oklahoma Health Sciences Center have the ability to perform this type of low pressure cell sorting sample collection. Across cell culture and animal model based research studies the ability to collect specific cellular populations for subsequent culturing, molecular, or biochemical analyses from complex mixtures of cells is critical. Being able to collect specific cell types has great advantages for understanding cellular function in health and disease and how different changes across multiple cell types work in a cell non-autonomous fashion to cause tissue dysfunction and disease. Traditionally, cell sorting techniques require specialized sheath fluids for suspension of cells, high pressure sorting, and complex instruments. A new approach to cell sorting uses microchip cartridges to perform all aspects of cell sorting without using high pressures or specialized sheath fluids. This creates a completely sterile and disposable environment for cell sorting that also treats the cells much more gently, increasing their viability for subsequent culturing or causing less molecular and biochemical changes for subsequent analyses. The Miltyeni Tyto instrument was selected based on the versatility of the instrument to meet the various needs of OKC VAMC investigators. The ability of this system to sort cells for a wide variety of applications including neuroscience, immunology, gastrointestinal and cancer research meets the needs of our varied investigators’ research areas. All of the investigators can make use of this cell sorting capabilities to subsequently sub-culture cells, or collect the cells for biochemical and molecular analyses. The ease of use, reduced maintenance, as compared to conventional cell sorters, complete sterility are advantages to core instrumentation used by a variety of groups. Also central to instrument selection was the three integrated lasers which will allow for up to ten parameter sorting of cells. A survey of all VA researchers at OKC VAMC showed a wide variety of researcher will utilize the system. This instrument will be used by five major user and two minor users who have five VA Merit grants funded and two senior career scientist awards as well as a number of future users.