ShEEP Request for Cell Sorter

ShEEP 请求细胞分选仪

• 批准号: 9906780
• 负责人: ARLAN G. RICHARDSON
• 金额: --
• 依托单位: OKLAHOMA CITY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9906780
• 关键词: Aging; Animal Model; Area; Award; Biochemical; Biomedical Research; Buffers; Cancer Immunology Science; Cell Culture Techniques; Cell Separation; Cell Survival; Cell physiology; Cell surface; Cells; Charge; Cities; Collecting Cell; Complex; Complex Mixtures; DNA Methylation; Detection; Disease; Environment; Functional disorder; Funding; Future; Gene Expression; Genomics; Grant; Health; Health Sciences; Immunology; Lasers; Liquid substance; Maintenance; Malignant neoplasm of gastrointestinal tract; Medical center; Minor; Mitochondria; Modality; Molecular; Molecular Analysis; Neurosciences; Neurosciences Research; Oklahoma; Population; Preparation; Process; Reporter Genes; Research; Research Personnel; Resolution; Sampling; Scientist; Sheep; Shock; Sorting - Cell Movement; Sterility; Stress; Surveys; Suspensions; System; Techniques; Technology; Tissues; Training; Transgenes; Universities; Work; anticancer research; base; career; cell type; epigenomics; flexibility; instrument; instrumentation; interest; marker transgenes; microchip; new technology; novel strategies; operation; population based; pressure; programs; research facility; research study; sample collection

Abstract We are requesting funds to purchase a Miltyeni Tyto cell sorting system. This instrument allows investigators to sort cells based on fluorescent markers from transgenes or from cell surface markers in a manner unlike conventional cell sorting systems. A number of technological advancements within this instrument increase the throughput, ease of use, sterility, and cell viability. Currently, neither the research facility at the Oklahoma City VA Medical Center (OKC VAMC) or the affiliated University of Oklahoma Health Sciences Center have the ability to perform this type of low pressure cell sorting sample collection. Across cell culture and animal model based research studies the ability to collect specific cellular populations for subsequent culturing, molecular, or biochemical analyses from complex mixtures of cells is critical. Being able to collect specific cell types has great advantages for understanding cellular function in health and disease and how different changes across multiple cell types work in a cell non-autonomous fashion to cause tissue dysfunction and disease. Traditionally, cell sorting techniques require specialized sheath fluids for suspension of cells, high pressure sorting, and complex instruments. A new approach to cell sorting uses microchip cartridges to perform all aspects of cell sorting without using high pressures or specialized sheath fluids. This creates a completely sterile and disposable environment for cell sorting that also treats the cells much more gently, increasing their viability for subsequent culturing or causing less molecular and biochemical changes for subsequent analyses. The Miltyeni Tyto instrument was selected based on the versatility of the instrument to meet the various needs of OKC VAMC investigators. The ability of this system to sort cells for a wide variety of applications including neuroscience, immunology, gastrointestinal and cancer research meets the needs of our varied investigators’ research areas. All of the investigators can make use of this cell sorting capabilities to subsequently sub-culture cells, or collect the cells for biochemical and molecular analyses. The ease of use, reduced maintenance, as compared to conventional cell sorters, complete sterility are advantages to core instrumentation used by a variety of groups. Also central to instrument selection was the three integrated lasers which will allow for up to ten parameter sorting of cells. A survey of all VA researchers at OKC VAMC showed a wide variety of researcher will utilize the system. This instrument will be used by five major user and two minor users who have five VA Merit grants funded and two senior career scientist awards as well as a number of future users.

摘要 我们正在申请购买Miltyeni Tyto细胞分选系统的资金。该仪器使调查人员能够 基于来自转基因的荧光标记或来自细胞表面标记的细胞以不同于 传统的细胞分选系统。该仪器中的一些技术进步增加了 产量、易用性、无菌和细胞活力。目前，俄克拉荷马城的研究机构 弗吉尼亚医学中心(OKC VAMC)或俄克拉荷马大学附属健康科学中心有能力 进行这种类型的低压细胞分选样品采集。以细胞培养和动物模型为基础 研究收集特定细胞群体用于后续培养、分子或 从复杂的细胞混合物中进行生化分析是至关重要的。能够收集特定类型的细胞具有很好的 了解健康和疾病中的细胞功能以及多个 细胞类型以细胞非自主方式工作，导致组织功能障碍和疾病。传统上，细胞 分选技术需要特殊的鞘液体来悬浮细胞、高压分选和复杂 乐器。一种新的细胞分选方法使用微芯片盒来执行细胞分选的所有方面 无需使用高压或专门的护套液。这创造了一个完全无菌和一次性的 细胞分选的环境，也更温和地对待细胞，提高它们的生存能力 培养的培养或引起较少的分子和生化变化以供后续分析的 Miltyeni Tyto仪器是根据仪器的多功能性来选择的，以满足各种需求 OKC VAMC调查人员。该系统能够对多种应用程序的细胞进行分类，包括 神经科学、免疫学、胃肠道和癌症研究满足了我们不同研究人员的需求 研究领域。所有的研究人员都可以利用这种细胞分选能力来随后进行继代培养 细胞，或收集细胞进行生化和分子分析。易用性，减少维护，如 与传统的细胞分选机相比，完全无菌是各种类型使用的核心仪器的优势 一群人。仪器选择的另一个核心是三个集成激光器，它将允许多达十个 单元格的参数排序。 OKC VAMC对所有退伍军人管理局研究人员的调查显示，将使用该系统的研究人员种类繁多。这 仪器将由五个主要用户和两个次要用户使用，他们有五个退伍军人奖励金和两个 资深职业科学家奖以及多位未来用户。