Understanding dynamical coding by NFkB

点击翻译按钮获取中文摘要

 DESCRIPTION (provided by applicant): Inflammation is critical for immunity but it is also a key determinant of a large number of human pathologies, if not as cause, then as promoter of progression. The expression of hundreds of genes is induced during inflammation, encompassing pro-inflammatory cytokines that function systemically, chemokines that recruit specific cell types to the site of infection, cell surface proteases for local tissue remodeling, itra-cellular innate immune effectors, regulators of cell survival and division, anti-inflammatory mediators and numerous signaling proteins that determine the progression of the inflammatory response and cellular responsiveness. Indeed, each pathologic threat, injury or inflammatory insult requires an appropriate and specific blend of these gene products to maximize the effectiveness of the inflammatory response and minimize its toxicity. Inflammatory responses are described as being stimulus-specific, mediated by stimulus-specific gene expression programs. It is well established that NFκB is the key regulator of inflammatory gene expression, and that the control of NFκB activity is highly dynamic due to several prominent delayed negative feedback loops. Prompted by our finding that NFκB dynamics are stimulus-specific, we posited the hypothesis that NFκB dynamics constitute a signaling code. According to this hypothesis, the dynamics of NFκB activity encode information about the stimulus that is then decoded by the nuclear gene regulatory network to produce stimulus-specific gene expression. While much progress has been made in elucidating the stimulus-encoding mechanisms, it has been remarkably difficult to develop a mechanistic understanding of how gene regulatory networks may decode stimulus-specific NFκB dynamics to produce stimulus-specific gene expression. Some reasons for the slow progress are the large numbers of possible stimulation conditions and the substantial cell-to-cell variability in NFκB dynamics - these necessitate high throughput single studies and novel data analysis approaches. Here we will combine (a) experimentally validated mathematical models (Hoffmann), (b) comprehensive microfluidics-enabled experimentation (Tay), and (c) new information theoretic approaches (Wollman) to (i) identify the dynamical features that mediate stimulus-specific cellular responses, (ii) quantify their reliability and information carrying capacity, and (iii) determine the gene regulatory strategies that decode these specific NFκB dynamical features. As a concerted collaborative effort, the proposed work will break through the current impasse in understanding how inflammatory gene expression programs are specified, thus revealing novel opportunities for therapeutic modulation in myriad pathologic settings. Further, as a model system for the field of signal transduction in general, the work will have broad impact in establishing concepts and workflow for the analysis of dynamical codes found also in other biological signaling systems.