Biochemisty, Pathology, and Imaging Core

生物化学、病理学和影像核心

• 批准号: 9188010
• 负责人: Ravichandran Ramasamy
• 金额: $ 23.06万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2018-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9188010
• 关键词: Acids; Adopted; Age; Albumins; Animals; Antibodies; Aorta; Apoptosis; Atherosclerosis; Biochemical; Biochemistry; Biological Assay; Biology; Buffers; Cardiovascular Diseases; Cells; Critiques; Cultured Cells; Darkness; Detection; Diabetes Mellitus; Diacetyl; Disease; Enzyme-Linked Immunosorbent Assay; Epitopes; Equipment; Fibronectins; Fluorescence; Fructose; Generations; Glucose; Glucose-6-Phosphate; Glucosephosphates; Glutathione; Glutathione Disulfide; Heart; High Pressure Liquid Chromatography; Homidium Bromide; Hour; Hydrogen Peroxide; Image; Imaging Techniques; Immunize; Immunoglobulin G; Immunohistochemistry; Incidence; Incubated; Individual; Instruction; Letters; Link; Lysine; Manufacturer Name; Measurement; Measures; Mediator of activation protein; Messenger RNA; Methods; Mitochondria; Modification; Molecular Probes; Monitor; Mus; Myocardial Infarction; NADPH Oxidase; Oryctolagus cuniculus; Oxidative Stress; Oxides; Oxis; Pathologic; Pathology; Perchloric Acids; Peripheral arterial disease; Permeability; Plant Roots; Plasma; Post-Translational Protein Processing; Procedures; Proteins; Publications; Publishing; Pyruvaldehyde; Reaction; Reader; Recommendation; Research; Sampling; Severities; Signal Transduction; Standardization; Sterility; Superoxides; System; Techniques; Time; Tissues; Vascular Diseases; Weight; Western Blotting; angiogenesis; catalase; diacetyldichlorofluorescein; ethyl lysine; experience; fluorophore; hydroethidine; imaging study; in vivo; methionine sulfoxide; molecular imaging; myocardial infarct sizing; novel; pentosidine; programs; receptor for advanced glycation endproducts; response

PROJECT SUMMARY (See instructions): Core B, the Biochemistry, Pathology and Imaging Core will continue to serve all three Projects through all five years of the Program. The core is composed of three units: (1) The Biochemistry Unit of Core B will perform assays on tissue/plasma of mice and cultured cells in the individual projects for assessment of oxidative stress, AGEs and other biochemical mediators linked to RAGE and vascular dysfunction. (2) The Pathology Unit of Core B will perform pathological analysis of mouse tissues. This Unit will serve all 3 projects for standardization of immunohistochemistry and semiquantitative analyses. The Unit will perform analysis of atherosclerosis (aortas (atherosclerosis at the aortic root and en face assessment of aorta), angiogenesis (Project 2-3) and myocardial infarction size (Project 3). (3) The newly-formed Imaging Unit will perform novel imaging techniques using state-of-the-art new equipment for molecular imaging studies in atherosclerosis and apoptosis (Project 1) and angiogenesis (Project 2).

项目摘要（参见说明）： 核心 B（生物化学、病理学和成像核心）将在该计划的五年内继续为所有三个项目提供服务。核心由三个单元组成：（1） 核心 B 的生物化学部门将在各个项目中对小鼠的组织/血浆和培养细胞进行检测，以评估氧化应激、AGE 以及与 RAGE 和血管功能障碍相关的其他生化介质。 (2) Core B的病理学单元将对小鼠组织进行病理分析。该单位将为免疫组织化学和半定量标准化的所有 3 个项目提供服务 分析。该科室将对动脉粥样硬化（主动脉（主动脉根部动脉粥样硬化和主动脉正面评估）、血管生成（项目 2-3）和心肌梗塞面积（项目 3）进行分析。 (3) 新成立的成像部门将使用最先进的新设备执行新颖的成像技术，用于动脉粥样硬化和细胞凋亡（项目1）和血管生成（项目2）的分子成像研究。