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World Reference Center for Emerging Viruses and Arboviruses (WRCEVA)

世界新兴病毒和虫媒病毒参考中心 (WRCEVA)

基本信息

批准号：
9210602
负责人：
Scott C Weaver
金额：
$ 92.49万
依托单位：
UNIVERSITY OF TEXAS MED BR GALVESTON
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-02-01 至 2021-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9210602
关键词：
Adopted Antibodies Antigenic Diversity Antigens Arboviruses Arthropods Biological Bioterrorism Chikungunya virus Clinical Collaborations Collection Communities Complementary DNA Coronaviridae Coronavirus Cryoelectron Microscopy Culicidae Databases Dengue Diagnosis Diagnostic Disease Outbreaks Ebola virus Ecology Electron Microscopy Ensure Epidemiology Evolution Family Foundations Funding Genetic Genetic Determinism Genetic Variation Genomics Genus Phlebovirus Goals Growth Hantavirus Health Hemorrhage Human Immune Sera In Vitro Infection Institution International Intervention Laboratories Location Medical Methods National Institute of Allergy and Infectious Disease New York Pathogenesis Pattern Phenotype Phylogenetic Analysis Play Principal Investigator Public Health Quality Control Reagent Recording of previous events Research Research Support Role Sampling Scientist Serological Severe Acute Respiratory Syndrome Ships Source Taxonomy Texas Therapeutic Training United States National Institutes of Health Universities Vaccine Design Vaccines Viral Viral Genome Virus Virus Diseases Yellow Fever Zoonoses anthropogenesis base biodefense deep sequencing effective intervention feeding human disease improved in vivo new technology next generation sequencing novel programs public health relevance repository therapeutic development tool transmission process vaccine development vector vector transmission virus identification weapons

项目摘要

DESCRIPTION (provided by applicant): New and emerging viruses and arboviruses represent increasing threats to human health, yet their mechanisms of emergence remain poorly understood, and effective interventions are not available for most. Research on their ecology, evolution, epidemiology, emergence mechanisms, diagnostics, and development of vaccines and therapeutics remain critical public health needs. The World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) comprises a comprehensive, diverse collection of over 6,700 virus strains in 21 families, as well as antisera, antigens and other reagents to enable research worldwide. Approximately 400 new virus strains are added each year, and 1000 viruses and reagents are shipped annually. The WRCEVA also maintains broad expertise in both novel and traditional approaches to virus identification and characterization, and assists with outbreak diagnosis. This proposal seeks to continue these WRCEVA activities in support of NIH-funded and other research on emerging viruses worldwide through 5 Specific Aims: 1. Maintain a comprehensive set of emerging viruses, arboviruses and associated reagents to support research and surveillance. The virus collection as well as antigens, antibodies and other reagents will be continually enhanced to capitalize on new technology, and cDNA clones of selected strains will be added. NextGen sequencing-based quality control practices will be implemented to ensure strain accuracy/purity. 2. Discover, isolate and characterize newly acquired viruses by using electron microscopy, next generation sequencing, and serologic methods to determine relationships and taxonomic assignments, and to assess in vitro and in vivo host range. Clinical and field samples as well as viral isolates will be received for identification and characterization, and added to the repository. Critical phenotypes of newly discovered viruses and strains will be assessed by using in vitro and in vivo infections. 3. Perform sequencing and phylogenetic analyses of selected virus groups to determine evolutionary histories and emergence mechanisms, patterns of spread and infection, and to rapidly determine the sources of new outbreaks. Key virus strains will undergo genomic sequencing to generate databases that can be exploited for the rapid determination of new outbreak sources, including potential bioterrorism. 4. Characterize recently discovered mosquito-specific viruses (MSVs) and determine their evolutionary history, impact on the transmission of arboviruses, and genetic determinants of host range. Selected arbovirus taxa that include mosquito-specific viruses will be studied to understand the genetic basis of their host range restriction and assess their potential as tools to interfere with arbovirus transmission 5. Train scientists in the identification and characterization of emerging viruses and arboviruses. To further enhance research efforts in the U.S. and worldwide as well as to leverage collaborations that feed our collections, basic training in virus identification and characterizaton will be provided to qualified scientists.