Cell Culture and Translational Studies Core

细胞培养和转化研究核心

• 批准号: 9307580
• 负责人: KAREN M RIDGE
• 金额: $ 31.83万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9307580
• 关键词: Acute Lung Injury; Adult Respiratory Distress Syndrome; Alveolar; Alveolar Macrophages; Animals; Biological; Biological Assay; Biological Preservation; Biological databases; Bone Marrow; Bronchoalveolar Lavage Fluid; Cell Culture Techniques; Cell Line; Cell physiology; Cells; Characteristics; Collaborations; Collection; Cryopreservation; Databases; Ensure; Epithelial; Epithelial Cells; Experimental Designs; Extracellular Matrix; Fibroblasts; Functional disorder; Generations; Hospitals; Human; Human Resources; Immunologics; Infection; Laboratories; Liquid substance; Lung; Medical; Methods; Mus; Nitrogen; Patients; Phenotype; Plasticizers; Process; Protocols documentation; Publications; Rattus; Reagent; Research; Research Personnel; Rodent; Sampling; Secondary Cell Culture; Serum; Specimen; Techniques; Translating; Translational Research; Update; cost; cost effectiveness; established cell line; experience; experimental study; macrophage; microorganism; tissue culture; translational study

Project Summary The Cell Culture and Translational Studies Core, designated as Core B, is a centralized facility that provides primary alveolar epithelial type 2 and type 1 cells and macrophages from humans, rats, and mice, as well as rat and mouse fibroblasts, to each of the three projects and to Core C. Core B also maintains secondary lung cell lines for the PPG. Centralization of the cell culture facilities will ensure that a continuous supply of high- quality alveolar epithelial and macrophage cells are available to each of the three projects. The Core personnel has extensive experience in the isolation of primary alveolar type 2 and type 1 cells and macrophages from human, rat, and mouse lungs, as well as general cell culture techniques. The consolidated cell culture facility provides an economical means of isolating and culturing primary and secondary cells. This translates into reduced overall costs (i.e., personnel, reagents, and animals) and more importantly maintains the quality of the cells used in research. Additionally, Core B will facilitate translational research by coordinating the collection, analysis, and generation of a database of biological samples from ARDS patients and distributing BALF samples to the project investigators to be used according to the research plan outlined in Projects 1, 2, and 3.

项目摘要 细胞培养和翻译研究核心，被指定为核心B，是一个集中的设施，提供 人、大鼠和小鼠的原代肺泡上皮2型和1型细胞和巨噬细胞 大鼠和小鼠成纤维细胞，分别到三个项目和核心C核心B也维持继发性肺 PPG的细胞系。细胞培养设施的集中化将确保持续供应高质量的 三个项目都有优质的肺泡上皮细胞和巨噬细胞。核心人员 在原代肺泡2型和1型细胞和巨噬细胞的分离方面有丰富的经验 人类、大鼠和小鼠的肺，以及一般的细胞培养技术。整合的细胞培养设施 为分离和培养原代和次级细胞提供了一种经济的方法。这将转化为 降低了总成本(即人员、试剂和动物)，更重要的是保持了 用于研究的细胞。此外，核心B将通过协调收集，促进翻译研究， 分析和生成ARDS患者的生物样本数据库并分发BALF 根据项目1、2和3中概述的研究计划将样本提供给项目调查人员使用。