National Xenopus Resource Center

国家爪蟾资源中心

• 批准号: 9070037
• 负责人: Robert M Grainger
• 金额: $ 65.88万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-12 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9070037
• 关键词: Achievement; Address; Amphibia; Animal Housing; Animals; Applied Research; Area; Arts; Back; Bioinformatics; Biological; Biological Models; Biomedical Research; Breeding; Cellular biology; Clustered Regularly Interspaced Short Palindromic Repeats; Communities; Community Services; Complex; Congenital Abnormality; Cryopreservation; Custom; Development; Diabetes Mellitus; Educational process of instructing; Educational workshop; Enhancement Technology; Ensure; Five-Year Plans; Funding; Gene Transfer Techniques; Genes; Genetic; Goals; Grant; Health; Housing; Image; Inbreeding; Individual; Institutes; Laboratories; Learning; Life; Malignant Neoplasms; Marines; Methods; Mission; Neurobiology; Oocytes; Physiology; Productivity; Rana; Regenerative Medicine; Research; Research Personnel; Resources; Services; Signal Transduction; Site; System; Techniques; Technology; Testing; Training; Transgenes; Transgenic Organisms; United States National Institutes of Health; Universities; Use of New Techniques; Visit; Wood material; Work; Xenopus; Xenopus laevis; animal resource; base; biological research; college; design; genome editing; high standard; human disease; improved; insight; interest; meetings; model organisms databases; mutant; nervous system disorder; new technology; novel; pathogen; repository; research facility; screening; sperm cell; tool; transcription activator-like effector nucleases

 DESCRIPTION (provided by applicant): Overall Component This project supports continued funding for the National Xenopus Resource (NXR) located at the Marine Biological Laboratory in Woods Hole, MA and identified as one of two top priorities for the Xenopus community. The NXR was established in 2010 to serve as a national resource for researchers working with the Xenopus amphibian model system, which includes two species Xenopus laevis and Xenopus tropicalis. The NXR serves as a national repository for community-generated animal stocks as well as serving as an advanced training venue that greatly facilitates productivity for a multitude of researchers. It is a community-oriented resource center that serves all Xenopus researchers, including individuals from large research-focused universities to small liberal arts colleges. As biological research becomes ever more complex, often requiring specialized animal lines and involving diverse technologies, individual laboratory units become insufficient to meet all of the demands required for addressing significant biological problems and a centralized repository or stock center becomes essential. There are five main aims to this grant. First, the NXR will maintain current stocks of frogs as well as obtain new lines making them available to the community; this includes special inbred, wild type and mutant lines of both species. In the second aim we outline the NXR custom research services for creating transgenic and mutant animals that are offered to the research community. In the third aim we outline the resources that we have created for the Xenopus community that helps to facilitate the use of new techniques in the community. These resources benefit from the excellent MBL facilities for research, teaching and housing. This includes the development of new advanced training workshops taught by experts in each field that serve to teach and propagate specialized techniques; this includes workshops on bioinformatics, imaging, genome editing, transgenesis and husbandry. In this aim we also outline the research hotel service, which provides opportunities for researchers to come to the MBL for short-term visits and use NXR resources. The last two aims are focused on applied research. In Aim 4 we describe new transgenic technology and enhancement of the new genome editing technology. In the last aim we propose to optimize sperm cryopreservation in X. laevis and develop a new health screening PCR service to test for the presence of specific pathogens.

 描述（由申请人提供）：总体组成部分 该项目支持继续为位于马萨诸塞州伍兹霍尔海洋生物实验室的国家非洲爪蟾资源 (NXR) 提供资金，并被确定为非洲爪蟾社区的两个首要任务之一。 NXR 成立于 2010 年，旨在为从事爪蟾两栖动物模型系统研究的研究人员提供国家资源，该系统包括非洲爪蟾和热带爪蟾两个物种。 NXR 是社区产生的动物种群的国家存储库，也是先进的培训场所，极大地提高了大众的生产力 的研究人员。它是一个面向社区的资源中心，为所有非洲爪蟾研究人员提供服务，包括从大型研究型大学到小型文理学院的个人。随着生物学研究变得越来越复杂，通常需要专门的动物品系并涉及不同的技术，单个实验室单位不足以满足解决重大生物学问题所需的所有需求，因此集中存储库或库存中心变得至关重要。这笔赠款有五个主要目的。首先，NXR 将维持现有的青蛙库存并获得新的品系，使它们可供社区使用；这包括两个物种的特殊近交系、野生型和突变系。在第二个目标中，我们概述了 NXR 定制研究服务，用于创建向研究界提供的转基因和突变动物。在第三个目标中，我们概述了我们为 Xenopus 社区创建的资源，这些资源有助于促进社区中新技术的使用。这些资源受益于 MBL 优良的研究、教学和住宿设施。这包括开发新的高级培训讲习班，由各个领域的专家讲授，以教授和传播专业技术；这包括生物信息学、成像、基因组编辑、转基因和畜牧业研讨会。为此，我们还概述了研究酒店服务，该服务为研究人员来 MBL 进行短期访问和使用 NXR 资源提供了机会。最后两个目标侧重于应用研究。在目标 4 中，我们描述了新的转基因技术和新基因组编辑技术的增强。在最后一个目标中，我们建议优化非洲虎精子冷冻保存，并开发一种新的健康筛查 PCR 服务来测试特定病原体的存在。