National Xenopus Resource Center

国家爪蟾资源中心

• 批准号: 7764306
• 负责人: Robert M Grainger
• 金额: $ 67.42万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-12 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7764306
• 关键词: Address; Advisory Committees; Affect; Animal Housing; Animal Husbandry; Animals; Area; Bioinformatics; Biological; Budgets; Cancer Biology; Cell Adhesion; Cell Cycle Regulation; Cells; Communities; Community Surveys; Congenital Abnormality; Core Facility; Databases; Development; Developmental Cell Biology; Disease Outbreaks; Educational process of instructing; Embryo; Female; Fertility; Funding; Gene Expression Regulation; Gene Transfer Techniques; Generations; Genetic; Goals; Guidelines; Health; Housing; Human Resources; Image; Laboratories; Leadership; Learning; Malignant - descriptor; Marines; Methods; Monitor; Movement; Paper; Pattern; Preparation; Process; Protocols documentation; Published Comment; Rana; Recovery; Regenerative Medicine; Research; Research Personnel; Research Priority; Resources; Role; Series; Shipping; Ships; Signal Transduction; Site; System; Technology; Time; Transgenic Animals; Transgenic Organisms; United States National Institutes of Health; Visit; Wood material; Xenopus; Xenopus laevis; base; biological research; cost; egg; genetic manipulation; imaging modality; improved; insight; mutant; new technology; operation; positional cloning; public health relevance; research study; web site

DESCRIPTION (provided by applicant): This project would establish a national Xenopus Resource Center at the Marine Biological Laboratory (MBL) in Woods Hole, MA. White Papers submitted to NIH by the Xenopus research community describe the urgent need for a center to house animal stocks and to disseminate new technology. Several laboratories, primarily those who are key personnel on this proposal, have defined the husbandry, health and housing conditions needed to develop a major stock center. The MBL is an ideal place for such a center because it is acknowledged as a premier site for biological research; it has a long association with the Xenopus research community and, as part of a Regenerative Medicine initiative, will fund the construction of a new Xenopus Center if funds are obtained for Center operation in 2009. This resubmission has four aims. The first entails setting up the facility in year 1 and generating critical animal lines (wildtype, inbred, transgenic and mutant). In aim 2, covering years 2-5, the scope of the facility will be expanded, to provide a spectrum of animal lines recognized as a high priority by the research community. In addition, transgenic and genetic core facilities will be established as part of the new Center to allow new lines to be generated, and for researchers to visit the facility to learn technologies associated with these areas. In the third aim, we focus on the role of the proposed Center in disseminating new technology This would include a series of minicourses to be offered at the Center, to be taught by experts in particular areas (e.g. husbandry, transgenesis, advanced imaging, preparation of egg extracts, bioinformatics). The final aim focuses on animal husbandry, include setting up protocols for enhanced husbandry and health surveillance, and disseminating these to the research community. Experiments are proposed to optimize husbandry to improve fertility of females and shorten the time to sexual maturity. Some key changes in this resubmission are: 1) an increased emphasis on X. laevis stocks (and concomitant reduction of X. tropicalis stocks) in order to better serve community needs; 2) development of a clearer leadership plan; 3) reduction in the complexity of aims (e.g. reducing stock number in the Center; removing forward genetics core); and 4) clarification of health and disease outbreak issues. PUBLIC HEALTH RELEVANCE (provided by applicant): Cell biologists studying Xenopus have made many breakthroughs in our understanding of cell adhesion and movements, signal transduction and control of the cell cycle, and thus to our understanding of cancer biology, because malignant transformation affects all of these processes in fundamental ways. Developmental biologists studying Xenopus have made transformative insights into processes controlling patterning of and movements in the embryo. and gene regulation, which underlie a multitude of birth defects.

描述（由申请人提供）：本项目将在马萨诸塞州伍兹霍尔海洋生物实验室（MBL）建立一个国家爪蟾资源中心。非洲爪蟾研究界提交给美国国立卫生研究院的白皮书描述了建立一个中心来饲养动物和传播新技术的迫切需要。几个实验室，主要是这项提案的关键人员，已经确定了发展一个主要牲畜中心所需的饲养、卫生和住房条件。MBL是这样一个中心的理想场所，因为它被公认为生物研究的首要场所；它与非洲爪蟾研究界有着长期的联系，作为再生医学倡议的一部分，如果2009年获得中心运营的资金，它将资助建设一个新的非洲爪蟾中心。这次重新提交有四个目的。第一种方法需要在第一年建立设施，并培育关键的动物品系（野生型、近交系、转基因和突变）。在目标2中，涵盖第2-5年，该设施的范围将扩大，以提供被研究界认为是高度优先的动物品系的光谱。此外，将建立转基因和遗传核心设施，作为新中心的一部分，以便产生新的品系，并供研究人员参观该设施，学习与这些领域相关的技术。在第三个目标中，我们关注拟议中的中心在传播新技术方面的作用，这将包括在中心提供一系列迷你课程，由特定领域的专家教授（例如，畜牧业，转基因，先进成像，蛋提取物制备，生物信息学）。最终目标集中在畜牧业，包括制定加强畜牧业和卫生监测的协议，并将这些协议传播给研究界。提出了优化饲养的实验，以提高雌性的生育能力，缩短性成熟时间。这次重新提交的一些关键变化是：1)增加了对热带野檀种群的重视（同时减少了热带野檀种群），以便更好地满足社区需求；2)制定更清晰的领导计划；3)降低目标的复杂性（例如减少中心的库存数量，去除前向遗传核心）；4)澄清健康和疾病暴发问题。