National Xenopus Resource Center

国家爪蟾资源中心

• 批准号: 7764306
• 负责人: Robert M Grainger
• 金额: $ 67.42万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-12 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7764306
• 关键词: Address; Advisory Committees; Affect; Animal Housing; Animal Husbandry; Animals; Area; Bioinformatics; Biological; Budgets; Cancer Biology; Cell Adhesion; Cell Cycle Regulation; Cells; Communities; Community Surveys; Congenital Abnormality; Core Facility; Databases; Development; Developmental Cell Biology; Disease Outbreaks; Educational process of instructing; Embryo; Female; Fertility; Funding; Gene Expression Regulation; Gene Transfer Techniques; Generations; Genetic; Goals; Guidelines; Health; Housing; Human Resources; Image; Laboratories; Leadership; Learning; Malignant - descriptor; Marines; Methods; Monitor; Movement; Paper; Pattern; Preparation; Process; Protocols documentation; Published Comment; Rana; Recovery; Regenerative Medicine; Research; Research Personnel; Research Priority; Resources; Role; Series; Shipping; Ships; Signal Transduction; Site; System; Technology; Time; Transgenic Animals; Transgenic Organisms; United States National Institutes of Health; Visit; Wood material; Xenopus; Xenopus laevis; base; biological research; cost; egg; genetic manipulation; imaging modality; improved; insight; mutant; new technology; operation; positional cloning; public health relevance; research study; web site

DESCRIPTION (provided by applicant): This project would establish a national Xenopus Resource Center at the Marine Biological Laboratory (MBL) in Woods Hole, MA. White Papers submitted to NIH by the Xenopus research community describe the urgent need for a center to house animal stocks and to disseminate new technology. Several laboratories, primarily those who are key personnel on this proposal, have defined the husbandry, health and housing conditions needed to develop a major stock center. The MBL is an ideal place for such a center because it is acknowledged as a premier site for biological research; it has a long association with the Xenopus research community and, as part of a Regenerative Medicine initiative, will fund the construction of a new Xenopus Center if funds are obtained for Center operation in 2009. This resubmission has four aims. The first entails setting up the facility in year 1 and generating critical animal lines (wildtype, inbred, transgenic and mutant). In aim 2, covering years 2-5, the scope of the facility will be expanded, to provide a spectrum of animal lines recognized as a high priority by the research community. In addition, transgenic and genetic core facilities will be established as part of the new Center to allow new lines to be generated, and for researchers to visit the facility to learn technologies associated with these areas. In the third aim, we focus on the role of the proposed Center in disseminating new technology This would include a series of minicourses to be offered at the Center, to be taught by experts in particular areas (e.g. husbandry, transgenesis, advanced imaging, preparation of egg extracts, bioinformatics). The final aim focuses on animal husbandry, include setting up protocols for enhanced husbandry and health surveillance, and disseminating these to the research community. Experiments are proposed to optimize husbandry to improve fertility of females and shorten the time to sexual maturity. Some key changes in this resubmission are: 1) an increased emphasis on X. laevis stocks (and concomitant reduction of X. tropicalis stocks) in order to better serve community needs; 2) development of a clearer leadership plan; 3) reduction in the complexity of aims (e.g. reducing stock number in the Center; removing forward genetics core); and 4) clarification of health and disease outbreak issues. PUBLIC HEALTH RELEVANCE (provided by applicant): Cell biologists studying Xenopus have made many breakthroughs in our understanding of cell adhesion and movements, signal transduction and control of the cell cycle, and thus to our understanding of cancer biology, because malignant transformation affects all of these processes in fundamental ways. Developmental biologists studying Xenopus have made transformative insights into processes controlling patterning of and movements in the embryo. and gene regulation, which underlie a multitude of birth defects.

描述（由申请人提供）：该项目将在马萨诸塞州伍兹霍尔市的海洋生物实验室（MBL）建立一个国家Xenopus资源中心。 Xenopus研究社区提交给NIH的白皮书描述了一个迫切需要一个中心容纳动物库存并传播新技术的中心。几个实验室，主要是该提案中的关键人员的实验室，已经定义了开发主要股票中心所需的饲养，健康和住房条件。 MBL是这样一个中心的理想场所，因为它被认为是生物学研究的首要地点。它与Xenopus研究社区有着悠久的联系，作为再生医学计划的一部分，如果在2009年获得中心运营的资金，将为新的Xenopus中心的建设提供资金。此重新提取的目标有四个目标。第一个需要在第1年内建立设施并产生关键的动物线（野生型，近交，转基因和突变体）。在AIM 2（涵盖2 - 5年的AIM 2）中，将扩大该设施的范围，以提供一系列动物线，并被研究界认为是高度优先的。此外，将建立转基因和遗传核心设施作为新中心的一部分，以允许生成新的线路，并供研究人员访问该设施以学习与这些领域相关的技术。在第三个目标中，我们关注拟议中心在传播新技术方面的作用，这将包括在中心提供的一系列微型库，由特定领域的专家（例如，饲养，转基因，高级成像，鸡蛋提取物的制备，生物学信息学）教授。最终目标的重点是畜牧业，包括制定加强饲养和健康监视的协议，并将其传播给研究社区。提出了实验来优化饲养以提高女性的生育能力，并缩短了性成熟的时间。此重新提交的一些关键变化是：1）越来越重视X. laevis股票（以及X. Tropicalis股票的减少），以便更好地满足社区需求； 2）制定更清晰的领导计划； 3）降低目标的复杂性（例如，中心的库存数量降低；去除前遗传学核心）； 4）澄清健康和疾病爆发问题。 公共卫生相关性（由申请人提供）：研究Xenopus的细胞生物学家在我们对细胞粘附和运动的理解，信号转导和对细胞周期的控制以及对我们对癌症生物学的理解的理解方面取得了许多突破，因为恶性转化会影响基本方式的所有这些过程。研究Xenopus的发展生物学家已经对控制胚胎中模式和运动的过程进行了变革性的见解。和基因调节，这是许多先天缺陷的基础。