National Xenopus Resource Center

国家爪蟾资源中心

• 批准号: 9269631
• 负责人: Robert M Grainger
• 金额: $ 63.58万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-12 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9269631
• 关键词: Achievement; Address; Amphibia; Animal Housing; Animals; Applied Research; Area; Arts; Back; Bioinformatics; Biological; Biological Models; Biomedical Research; Breeding; Cellular biology; Clustered Regularly Interspaced Short Palindromic Repeats; Communities; Community Services; Complex; Congenital Abnormality; Cryopreservation; Custom; Development; Diabetes Mellitus; Educational process of instructing; Educational workshop; Ensure; Five-Year Plans; Funding; Gene Transfer Techniques; Genes; Genetic; Goals; Grant; Health; Housing; Image; Inbreeding; Individual; Institutes; Laboratories; Learning; Malignant Neoplasms; Marines; Methods; Mission; Neurobiology; Oocytes; Physiology; Productivity; Rana; Regenerative Medicine; Research; Research Personnel; Resources; Services; Signal Transduction; Site; System; Techniques; Technology; Testing; Training; Transgenes; Transgenic Organisms; United States National Institutes of Health; Universities; Use of New Techniques; Visit; Wood material; Xenopus; Xenopus laevis; animal resource; base; biological research; college; design; genome editing; high standard; human disease; improved; insight; interest; meetings; model organisms databases; mutant; nervous system disorder; new technology; novel; pathogen; public health relevance; repository; research facility; screening; sperm cell; tool; transcription activator-like effector nucleases

 DESCRIPTION (provided by applicant): Overall Component This project supports continued funding for the National Xenopus Resource (NXR) located at the Marine Biological Laboratory in Woods Hole, MA and identified as one of two top priorities for the Xenopus community. The NXR was established in 2010 to serve as a national resource for researchers working with the Xenopus amphibian model system, which includes two species Xenopus laevis and Xenopus tropicalis. The NXR serves as a national repository for community-generated animal stocks as well as serving as an advanced training venue that greatly facilitates productivity for a multitude of researchers. It is a community-oriented resource center that serves all Xenopus researchers, including individuals from large research-focused universities to small liberal arts colleges. As biological research becomes ever more complex, often requiring specialized animal lines and involving diverse technologies, individual laboratory units become insufficient to meet all of the demands required for addressing significant biological problems and a centralized repository or stock center becomes essential. There are five main aims to this grant. First, the NXR will maintain current stocks of frogs as well as obtain new lines making them available to the community; this includes special inbred, wild type and mutant lines of both species. In the second aim we outline the NXR custom research services for creating transgenic and mutant animals that are offered to the research community. In the third aim we outline the resources that we have created for the Xenopus community that helps to facilitate the use of new techniques in the community. These resources benefit from the excellent MBL facilities for research, teaching and housing. This includes the development of new advanced training workshops taught by experts in each field that serve to teach and propagate specialized techniques; this includes workshops on bioinformatics, imaging, genome editing, transgenesis and husbandry. In this aim we also outline the research hotel service, which provides opportunities for researchers to come to the MBL for short-term visits and use NXR resources. The last two aims are focused on applied research. In Aim 4 we describe new transgenic technology and enhancement of the new genome editing technology. In the last aim we propose to optimize sperm cryopreservation in X. laevis and develop a new health screening PCR service to test for the presence of specific pathogens.

 描述（由适用提供）：总体组件该项目支持位于马萨诸塞州伍兹霍尔市海洋生物实验室的国家Xenopus资源（NXR）的持续资金，并被确定为Xenopus社区的两个主要优先事项之一。 NXR成立于2010年，是为使用Xenopus两栖动物模型系统的研究人员提供的国家资源，其中包括两个物种Xenopus laevis和xenopus tropicalis。 NXR是社区生成的动物股票的国家存储库，并充当了高级培训场地，可为众多的生产力提供极大的生产力 它是一个面向社区的资源中心，为所有Xenopus研究人员提供服务，包括从大型研究的大学到小型文科学院的个人。随着生物学研究变得越来越复杂，经常需要专门的动物线并涉及潜水员技术，单个实验室单位不足以满足解决重大生物学问题所需的所有需求，而集中的存储库或股票中心变得至关重要。这笔赠款有五个主要目标。首先，NXR将维护当前的青蛙库存，并获得新的产品线，使其可以向社区提供。这包括两种物种的特殊近交，野生型和突变线。在第二个目标中，我们概述了NXR定制研究服务，用于创建提供给研究社区的转基因和突变动物。在第三个目标中，我们概述了为Xenopus社区创造的资源，这些资源有助于促进社区中新技术的使用。这些资源受益于研究，教学和住房的出色MBL设施。这包括开发由专家在每个领域教授的新高级培训研讨会，这些研讨会有助于教授和传播专业技术；这包括有关生物信息学，成像，基因组编辑，转基因和畜牧业的研讨会。在此目标中，我们还概述了研究酒店服务，该服务为研究人员提供了短期访问并使用NXR资源的机会。最后两个目标集中在应用研究上。在AIM 4中，我们描述了新的转基因技术和新基因组编辑技术的增强。在最后一个目的中，我们建议在X. laevis中优化精子冷冻保存，并开发新的健康筛查PCR服务，以测试特定病原体的存在。