Developing plant synthetic biology platforms to elucidate the role of natural products
开发植物合成生物学平台以阐明天然产物的作用
基本信息
- 批准号:9371803
- 负责人:
- 金额:$ 9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-08-01 至 2018-06-30
- 项目状态:已结题
- 来源:
- 关键词:AddressAdvisory CommitteesAffectAnabolismAwardBiochemicalBiologicalBiological AvailabilityBiological ProcessBiological SciencesBiologyBrassicaBroccoli - dietaryCarbonCollaborationsCommunitiesComplexComplex MixturesConflict (Psychology)ConsumptionDevelopmentDietDiseaseEdible PlantsEducational workshopEngineeringEnsureEnvironmentEnzymesEscherichia coliEvolutionFacultyFamilyFellowshipFoundationsFutureGenerationsGlucosinolatesGoalsHealthHealth BenefitHumanHydrolysisIndividualIndole AlkaloidsIndolesInstitutesInstitutionInterdisciplinary StudyIntestinesJointsLaboratoriesLearningLinkMalignant NeoplasmsMass Spectrum AnalysisMeasuresMentorsMentorshipMetabolicMetabolic PathwayMetabolismMethodsMolecularMusNatural ProductsNicotianaNutritionalOrganismPathway interactionsPharmacologic SubstancePhotosynthesisPhylogenetic AnalysisPhysiologicalPhytochemicalPlant ComponentsPlant SourcesPlantsPlayPositioning AttributeProcessProductionPropertyResearchRiceRoleSchoolsScientistSulfurSystemTechniquesTechnologyTimeTobaccoTrainingTransgenic OrganismsTranslational ResearchUniversitiesVegetablesWorkYeastsantimicrobialantimicrobial drugbasebiological systemsbiosynthetic productcancer preventioncarbon fixationcareercareer developmentdesigndesign and constructionexperienceexperimental studyfeedinggut microbiomegut microbiotaimprovedinsightinterestmeetingsmembermetabolic engineeringmicrobialmicrobial communitymicrobiomenovelnutritionpathogenplant growth/developmentprogramsresearch and developmentscreeningsmall moleculestemsynthetic biologytooltool developmenttraitvisiting scholar
项目摘要
Project Summary and Abstract
Candidate and Environment
During my graduate career at UC Berkeley, I became interested in the evolution and engineering of metabolic
pathways, primarily relating to photosynthesis. It was through this work that I became familiar with the field of synthetic
biology and integrated it into my graduate work through engineering synthetic carbon fixation pathways to improve
photosynthetic yield and the synthesis of phylogenetically predicted enzymes that existed hundreds of millions of years
ago. Excited about the prospects of synthetic biology in plants, I received a Life Sciences Research Foundation
postdoctoral fellowship and joined Dr. Dominique Loqué's lab at the Joint BioEnergy Institute and Lawrence Berkeley
National Laboratory, where I have focused on developing plant synthetic biology tools to facilitate metabolic engineering
in plants. Although I have spent my postdoctoral career focused on tool development, I am interested in the application of
these technologies to engineer novel natural product biosynthetic pathways with unique biological functions. My long-
term career goals involve the development of synthetic biology tools and platforms for enabling basic discovery and
translational research involving plant natural products and metabolic engineering. My diverse research experiences
provide a strong foundation to attain this goal. Although I have spent much of my career focusing on primary carbon
metabolism and photosynthesis, I have not yet had any training in secondary metabolism and elucidation of natural
product biosynthetic pathways. Thus, my immediate goal is to obtain training from the K99/R00 award under the
guidance of experts in the field to successfully bridge my interests in synthetic biology and plant natural products and
ultimately transition to an independent faculty position.
My mentoring team is composed of experts in disparate yet complementary fields, allowing me to receive training
in their respective niches and organically build my own independent research program. I will be co-mentored by Dr.
Dominique Loqué (Lawrence Berkeley National Lab/UC Berkeley), an expert in plant synthetic biology, Dr. Elizabeth
Sattely (Stanford), a leading expert in plant secondary metabolism, and Dr. Jay Keasling (Lawrence Berkeley National
Lab/UC Berkeley), a pioneer in microbial synthetic biology and metabolic engineering. I will receive further training and
guidance form Dr. Justin Sonnenburg (Stanford) as a collaborator and expert studying the role of small molecules on the
intestinal microbiota. I will have regular one-on-one meetings with my advisory committee to ensure that I stay on track
with my career development and research progress to obtain an independent faculty position.
The proximity between both Berkeley and Stanford will enable me to take advantage of the excellent academic
environment of both institutions, providing opportunities to meet visiting scholars, attend seminars, and take courses from
experts in the field. Furthermore, leading experts in the field of synthetic biology and plant biology are members of both
universities, providing opportunities to receive guidance and input on research from other distinguished faculty. I will take
advantage of seminars and workshops on career and professional development offered by both schools. The combination
of mentorship and professional training between Berkeley and Stanford will provide the training needed to establish my
own unique interdisciplinary research program merging synthetic biology and plant secondary metabolism.
Research
Plants produce a wealth of natural products that have wide-ranging effects on human nutrition, disease, and overall
wellbeing. However, because of the complexities of many of these specialized metabolites, we have been limited in our
ability to study the effects of individual phytochemicals on human health. Recently, the nascent field of synthetic biology
has provided the means to dissect biological systems into their individual components, enabling scientists to reverse
engineer and reconstruct their biochemical makeup. This approach has largely been limited to simple organisms (e.g., E.
coli and yeast); however, plants provide a unique platform to leverage synthetic biology. My research focuses on
introducing design and engineering principles to rationally manipulate plant metabolism in order to investigate the
biosynthesis and physiological roles of plant natural products. Edible cruciferous plants (e.g., broccoli, bok choy) have
been implicated in cancer prevention, stemming from their diversity of indole glucosinolate derivatives. However,
because of the vast diversity of bioactive compounds produced in cruciferous plants, it is challenging to tease apart and
pinpoint the specific molecules that may be responsible for a trait as complex as cancer prevention. As a result, many
studies have resulted in conflicting findings and tenuous links between glucosinolates and their claimed nutritional
benefits. Engineering specific target molecules into novel hosts with no basal biological activity may provide insight and
help clarify their role in human health at a molecular level. The development of plant synthetic biology platforms to
produce and deliver specific concentrations of target plant natural products will enable future studies to more
quantitatively study the claimed benefits and effects of glucosinolates on human health.
项目摘要及摘要
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Towards a sustainable bio-based economy: Redirecting primary metabolism to new products with plant synthetic biology.
- DOI:10.1016/j.plantsci.2018.03.012
- 发表时间:2018-08
- 期刊:
- 影响因子:0
- 作者:Shih PM
- 通讯作者:Shih PM
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Patrick Shih其他文献
Patrick Shih的其他文献
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{{ truncateString('Patrick Shih', 18)}}的其他基金
Developing plant synthetic biology platforms to elucidate the role of natural products
开发植物合成生物学平台以阐明天然产物的作用
- 批准号:
9769625 - 财政年份:2017
- 资助金额:
$ 9万 - 项目类别:
Developing plant synthetic biology platforms to elucidate the role of natural products
开发植物合成生物学平台以阐明天然产物的作用
- 批准号:
10464431 - 财政年份:2017
- 资助金额:
$ 9万 - 项目类别:
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