Developing plant synthetic biology platforms to elucidate the role of natural products

开发植物合成生物学平台以阐明天然产物的作用

• 批准号: 10464431
• 负责人: Patrick Shih
• 金额: $ 22.04万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10464431
• 关键词: Developing; plant; synthetic; biology; platforms

Project Summary and Abstract No change to the Project Summary and Abstrasct will be made with the transfer of this award from UC Davis to UC Berkeley. The change in recipient institution is not related to concerns about safety and/or work environments (e.g. due to concerns about harassment, bullying, retaliation, or hostile working conditions) involving the PD/PI. Plants produce a wealth of natural products that have wide-ranging effects on human nutrition, disease, and overall wellbeing. However, because of the complexities of many of these specialized metabolites, we have been limited in our ability to study the effects of individual phytochemicals on human health. Recently, the nascent field of synthetic biology has provided the means to dissect biological systems into their individual components, enabling scientists to reverse engineer and reconstruct their biochemical makeup. This approach has largely been limited to simple organisms (e.g., E. coli and yeast); however, plants provide a unique platform to leverage synthetic biology. My research focuses on introducing design and engineering principles to rationally manipulate plant metabolism in order to investigate the biosynthesis and physiological roles of plant natural products. Edible cruciferous plants (e.g., broccoli, bok choy) have been implicated in cancer prevention, stemming from their diversity of indole glucosinolate derivatives. However, because of the vast diversity of bioactive compounds produced in cruciferous plants, it is challenging to tease apart and pinpoint the specific molecules that may be responsible for a trait as complex as cancer prevention. As a result, many studies have resulted in conflicting findings and tenuous links between glucosinolates and their claimed nutritional benefits. Engineering specific target molecules into novel hosts with no basal biological activity may provide insight and help clarify their role in human health at a molecular level. The development of plant synthetic biology platforms to produce and deliver specific concentrations of target plant natural products will enable future studies to more quantitatively study the claimed benefits and effects of glucosinolates on human health.

项目摘要和摘要 将该奖项从加州大学戴维斯分校移交给加州大学戴维斯分校时，不会对项目摘要和摘要进行任何更改 伯克利。接受机构的变化与对安全和/或工作环境的担忧无关(例如，由于 对骚扰、欺凌、报复或敌对工作条件的担忧)涉及PD/PI。 植物产生丰富的天然产物，对人类的营养、疾病和整体都有广泛的影响 幸福。然而，由于许多这些专门的代谢物的复杂性，我们在我们的 研究个别植物化学物质对人类健康的影响的能力。最近，合成生物学这一新兴领域 提供了将生物系统分解为各自组成部分的方法，使科学家能够逆转 设计并重建他们的生化组成。这种方法在很大程度上局限于简单的生物体(例如，E。 大肠杆菌和酵母)；然而，植物为利用合成生物学提供了一个独特的平台。我的研究重点是 介绍合理操作植物新陈代谢的设计和工程原理，以研究 植物天然产物的生物合成和生理作用。可食用的十字花科植物(如西兰花、白菜) 由于其吲哚硫代葡萄糖苷衍生物的多样性，被认为与癌症预防有关。然而， 由于十字花科植物中产生的生物活性化合物种类繁多，要将它们分离出来是一件具有挑战性的事情。 找出可能对癌症预防这样复杂的特征负责的特定分子。因此，许多人 研究发现硫代葡萄糖苷与其声称的营养成分之间存在相互矛盾的联系。 福利。将特定的靶分子工程到没有基本生物活性的新宿主中可能提供洞察力和 有助于在分子水平上阐明它们在人类健康中的作用。植物合成生物学平台的开发 生产和交付特定浓度的目标植物天然产物将使未来的研究能够获得更多 定量研究硫代葡萄糖酸盐声称的益处和对人类健康的影响。