XANES OF ZINC BINDING TO CARDIAC PROTEINS

锌的 XAN 与心脏蛋白质的结合

• 批准号: 8361294
• 负责人: BRADLEY M PALMER
• 金额: $ 0.2万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361294
• 关键词: Binding; Biophysics; Cardiac; Data; Effectiveness; Film; Freeze Drying; Funding; Grant; Heart; Methods; National Center for Research Resources; Principal Investigator; Proteins; Rattus; Research; Research Infrastructure; Resources; Sampling; Slice; Sorting - Cell Movement; Source; Spectrum Analysis; Thick; Tissues; United States National Institutes of Health; Zinc; cost; research study; tissue preparation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We prepared thin slices of rat heart tissue (~50 microns thick), freeze dried and placed into an ultralene thin film tissue holder. The sample were irradiated at 12 keV for purposes of detect the elemental content distribution and determine the Zn content for spectroscopy purposes. These experiments evaluated the efficacy of this method with this sort of tissue and tissue preparation. We found that the method can detect enough amount of Zn to allow performing XANES at Zn K edge with sufficient effectiveness. This experiment also allowed us to collect preliminary data that will be used to plan next experiments.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们制备大鼠心脏组织的薄片（约50微米厚），冷冻干燥并置于ultralene薄膜组织保持器中。在12 keV下照射样品，以检测元素含量分布并确定Zn含量以用于光谱学目的。这些实验评估了这种方法对这种组织和组织制备的有效性。我们发现，该方法可以检测到足够量的锌，以允许执行XANES在锌K边缘具有足够的有效性。该实验还使我们能够收集初步数据，这些数据将用于计划下一个实验。