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The Role of 2-Pore Domain Potassium Channels in Acute Lung Injury.

2 孔域钾通道在急性肺损伤中的作用。

基本信息

批准号：
9272423
负责人：
Andreas Schwingshackl
金额：
$ 17.39万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-02-01 至 2019-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9272423
关键词：
Acute Lung Injury Advisory Committees Affect Alveolar Award Bronchoalveolar Lavage Fluid Budgets Clinical Collaborations Coupling Development Disease Dyes Electrical Resistance Electrophysiology (science)Environment Environmental air flow Epithelial Epithelial Cell Proliferation Epithelial Cells Evans blue stain Exposure to Financial Support Functional disorder Funding Goals Healthcare Human Hyperoxia Image Immunohistochemistry In Vitro Inflammation Mediators Inflammatory Intensive Care International Intervention Knockout Mice Life Literature Lung Measurement Measures Mechanical ventilation Mechanics Medicine Mentorship Molecular Morbidity - disease rate Mus Outcome Oxygen Oxygen Therapy Care Pathogenesis Pathogenicity Pathway interactions Patients Permeability Phosphorylation Potassium Potassium Channel Protein Isoforms Proteins Publications Pulmonology Rattus Regulation Reporting Research Research Infrastructure Role Savings Signal Transduction Slice Speed Stimulus Stretching TNF gene Techniques Therapeutic Therapeutic Intervention Tight Junctions Transducers Water base career cytokine electric impedance experimental study improved in vivo in vivo Model inhibitor/antagonist innovation interest knock-down lung injury mechanotransduction meetings monolayer mortality new therapeutic target novel novel therapeutic intervention overexpression patch clamp pressure public health relevance response sensor success treatment strategy

项目摘要

DESCRIPTION (provided by applicant): Despite advances in Pulmonology and Intensive Care Medicine, mortality rates of patients with Acute Lung Injury (ALI) remain high, perhaps because the underlying mechanisms of this disease are poorly understood. Patients with ALI routinely require high oxygen concentrations and positive-pressure ventilation, although both therapies accentuate ongoing lung injury. While the signaling cascades activated by hyperoxia are well known, "mechano-transduction" after alveolar distension is poorly understood. Recent literature suggests that 2-pore domain potassium (K2P) channels may act as mechano-sensors and mechano-transducers, and participate in stimulus-secretion coupling. However, little is known about the expression and potential functions of K2P channels in the lung. We propose a bold and novel hypothesis that K2P channels are expressed in lung epithelial cells, and that pathogenic K2P channel regulation caused by hyperoxia, mechanical stretch and TNF-a exposure, an environment similar to the one encountered in ALI, results in dysregulation of inflammatory mediator secretion from epithelial cells, and in loss of epithelial barrier function, two hallmarks of ALI. Our overarching objective is to investigate the mechanisms leading to hyperoxia- and mechanical stretch- induced lung injury, and to identify K2P channels as a new target in the search for innovative therapeutic strategies against ALI. Specifically, we will use both in vitro and in vivo approaches including K2P knockout mice to [1] investigate the effects of hyperoxia, mechanical stretch and TNF-a on K2P channel expression and function in cultured mouse and primary rat and human alveolar epithelial cells using molecular techniques, immunohistochemistry and patch clamp studies, [2] to determine the role of K2P channels in inflammatory mediator secretion from cultured and primary alveolar epithelial cells, and in broncho-alveolar lavage fluid from K2P knockout mice, and [3] to demonstrate that K2P channels regulate epithelial barrier function via Ca2+-dependent tight junction phosphorylation. We have the unique expertise and technical capabilities to study the effects of hyperoxia and mechanical stretch in both in vitro and in vivo models of ALI. In addition, Dr. Jaggar has an inimitable setup to measure global and localized intracellular Ca2+ concentrations. The academic environment at UTHSC, the outstanding mentorship, rich opportunities for collaborations, and the institutional, departmental, and divisional commitment to my research success provide the intellectual infrastructure and the financial support to guarantee my progress towards independent research funding, including an R01 award, within 5 years. These long-term goals will be achieved by targeting a minimum of 2 publications and 2 abstract presentations per year at international meetings, supplemented by formal coursework, and the close mentorship of Dr. Waters, Dr. Anand and my Career Advisory Committee.

描述（由申请人提供）：尽管肺病学和重症监护医学取得了进展，但急性肺损伤（ALI）患者的死亡率仍然很高，这可能是因为人们对这种疾病的潜在机制知之甚少。ALI患者通常需要高氧浓度和正压通气，尽管这两种治疗都加重了持续的肺损伤。虽然高氧激活的信号级联是众所周知的，但肺泡膨胀后的“机械转导”却知之甚少。最近的文献表明，2孔结构域钾（K2P）通道可能作为机械传感器和机械传感器，并参与刺激-分泌耦合。然而，对肺中K2P通道的表达和潜在功能知之甚少。我们提出了一个大胆而新颖的假设，即K2P通道在肺上皮细胞中表达，并且高氧、机械拉伸和TNF-a暴露引起的致病性K2P通道调节，类似于ALI所遇到的环境，导致上皮细胞炎症介质分泌失调，上皮屏障功能丧失，这是ALI的两个标志。我们的总体目标是研究导致高氧和机械拉伸诱导的肺损伤的机制，并确定K2P通道作为寻找针对ALI的创新治疗策略的新靶点。具体来说，我们将使用体外和体内方法，包括K2P敲除小鼠来研究高氧、机械拉伸和TNF-a对培养小鼠、原代大鼠和人肺泡上皮细胞中K2P通道表达和功能的影响，使用分子技术、免疫组织化学和膜片钳研究，确定K2P通道在培养和原代肺泡上皮细胞中炎症介质分泌中的作用。在K2P敲除小鼠的支气管肺泡灌洗液和[3]中，证明K2P通道通过Ca2+依赖性紧密连接磷酸化调节上皮屏障功能。我们有独特的专业知识和技术能力来研究高氧和机械拉伸对ALI体外和体内模型的影响。此外，Jaggar博士有一个独特的设置来测量全局和局部细胞内Ca2+浓度。清华大学的学术环境、优秀的导师、丰富的合作机会，以及学院、部门和分部对我研究成功的承诺，为我提供了智力基础设施和财政支持，以保证我在5年内获得独立研究资金，包括R01奖。这些长期目标将通过每年在国际会议上至少发表2篇论文和2篇摘要报告来实现，并辅以正式课程，以及沃特斯博士、阿南德博士和我的职业咨询委员会的密切指导。