Protein Quality Early in Life: Mechanisms of Growth and Later Obesity Development

生命早期的蛋白质质量：生长和后期肥胖发展的机制

• 批准号: 9352844
• 负责人: Minghua Tang
• 金额: $ 13.4万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-14 至 2020-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9352844
• 关键词: ATP phosphohydrolase; Achievement; Affect; Age-Months; Amino Acids; Arginine; Bacteroidetes; Biological Markers; Biometry; Birth; Body Composition; Body fat; Branched-Chain Amino Acids; Child Nutrition; Clinical Skills; Colorado; Complementary Feeding; Data; Data Analyses; Development; Diet; Dietary Proteins; Domestic Fowls; Early Intervention; Environment; Epidemiology; Exposure to; Faculty; Fatty acid glycerol esters; Fermentation; Fishes; Food; Future; Genes; Glycoside Hydrolases; Goals; Growth; Harvest; Homeostasis; Impairment; Individual; Infant; Institution; Insulin Resistance; Insulin-Like Growth Factor I; Investigation; Iron; Knowledge; Leadership; Life; Literature; Long-Term Effects; Marketing; Meat; Mediator of activation protein; Medical; Mentors; Metagenomics; Microbe; Micronutrients; Mission; Monitor; Obesity; Outcome; Overweight; Participant; Pattern; Play; Positioning Attribute; Postdoctoral Fellow; Prevention; Proteins; Protocols documentation; Public Health; Randomized Controlled Trials; Recommendation; Regulation; Research; Research Methodology; Research Training; Ribosomal RNA; Risk; Risk Factors; Role; Sampling; Serum; Solid; Source; Statistical Methods; Taxonomy; Techniques; Time; Toddler; Training; Training Programs; United States National Institutes of Health; Universities; Weight; Weight Gain; Zinc; acylcarnitine; base; career; career development; cohort; doubly-labeled water; effective intervention; evidence base; experience; feeding; gut microbiome; heart disease risk; human disease; insulin sensitivity; meetings; metabolomics; metagenomic sequencing; microbiome; modifiable risk; multidisciplinary; nutrition; obesity in children; obesity prevention; obesogenic; prevent; programs; protein intake; randomized trial; research and development; skills; sound; total energy expenditure; trimethyloxamine

ABSTRACT Rapid weight gain during the first two years of life predisposes to later-in-life obesity. However, there is a fundamental gap in knowledge of what dietary recommendations should be provided to infants and toddlers to promote optimal growth and prevent rapid weight gain. Dietary protein intake is considered a risk factor because it accelerates weight and fat gain in infants and toddlers, based primarily on research of dairy protein. However, the underlying mechanisms are not clear. These findings have been the basis of recent recommendations to reduce protein intake from birth to 24 months. What is not known is whether all protein sources have the same weight-accelerating effect. Our research group conducted a randomized controlled trial in infants consuming a meat- or dairy-based complementary diet from 6 to 12 months of age. Growth data showed that a dairy-based complementary diet, not meat, leads to impaired linear growth and increased risk for being overweight. The objective of this proposed project is to investigate the underlying mechanisms and determine which mediators are predictive for the different growth patterns in this cohort via fecal microbiome/metagenomics, serum metabolomics and doubly labeled water technique. Although the candidate has expertise in pediatric nutrition and conducting randomized controlled trials, training in mechanistic investigations of obesity development and research methodologies are critical to achieve the long-term goal of becoming a leader in pediatric nutrition focusing on implementing effective interventions early in life for optimal growth and obesity prevention. This career development application proposes a 4-year multidisciplinary training program to the candidate, who is a 4th year postdoctoral fellow transitioning to a junior faculty position. A well-developed training plan by the candidate and the mentoring team is in place, including 1) Hands-on experience of microbiome/metagenomics, metabolomics analysis and data interpretation; 2) Hands-on experience of conducting the doubly labeled water protocol for body composition and total energy expenditure in infants and toddlers, plus sample analysis and data interpretation; 3) Regular meetings with mentors to monitor project progress, achievements towards the short- and long-term goals, and didactic trainings; 4) Advanced coursework in microbiome, metabolomics and biostatistics; 5) Training in leadership and program management. University of Colorado Anschutz Medical Campus is world-leading research institution and provides the candidate an excellent research and training environment which facilitates a successful transition to an independent career in pediatric nutrition and early-in-life obesity prevention.

摘要 在生命的头两年体重迅速增加容易导致以后的肥胖。但有一个 在应该向婴幼儿提供什么样的饮食建议方面存在根本性的知识空白 以促进最佳生长并防止体重快速增加。膳食蛋白质摄入被认为是一个危险因素 因为它会加速婴儿和幼儿的体重和脂肪增加，主要是基于对乳制品蛋白质的研究。 然而，其内在机制尚不清楚。这些发现是最近 建议从出生到24个月减少蛋白质摄入量。目前尚不清楚的是， 源具有相同的重量加速效应。我们的研究小组进行了一项随机对照的 在6至12个月的婴儿中进行的试验，这些婴儿食用以肉类或乳制品为基础的补充饮食。增长 数据显示，以乳制品为基础的补充饮食，而不是肉类，会导致线性生长受损， 超重的风险。本项目的目的是研究潜在的机制， 确定哪些介质可预测该队列中的不同生长模式， 微生物组/宏基因组学、血清代谢组学和双标记水技术。虽然 候选人具有儿科营养和进行随机对照试验的专业知识， 肥胖发展的机制研究和研究方法对于实现 长期目标是成为儿科营养领域的领导者，重点是实施有效的干预措施 在生命的早期，以获得最佳的生长和预防肥胖。 这份职业发展申请向候选人提出了一个为期4年的多学科培训计划， 他是一名四年级的博士后研究员，正在向初级教师职位过渡。完善的培训计划 由候选人和辅导团队提供，包括1） 微生物组学/宏基因组学、代谢组学分析和数据解释; 2） 进行婴儿身体成分和总能量消耗的双标记水方案 和幼儿，加上样本分析和数据解释; 3）与导师定期会议，以监测 项目进展情况、短期和长期目标的实现情况以及教学培训情况; 4）高级 微生物组学，代谢组学和生物统计学课程; 5）领导力和计划培训 管理科罗拉多大学安舒茨医学院是世界领先的研究机构， 为候选人提供一个良好的研究和培训环境，促进成功的 过渡到儿科营养和早期肥胖预防的独立职业。