Protein Deamidation in Innate Immune Evasion Regulated by Viral Pseudo Enzymes

病毒伪酶调节的先天免疫逃避中的蛋白质脱酰胺

• 批准号: 9381580
• 负责人: Pinghui Feng
• 金额: $ 45.48万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-02 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9381580
• 关键词: Affinity Chromatography; Antiviral Agents; Binding; Biological Process; Complex; DNA Viruses; Data; Detection; Double-Stranded RNA; Enzymes; Epithelial; Epithelial Cells; Family; Future; Glean; Glutamine; Herpesviridae; Herpesviridae Infections; Human; Human Herpesvirus 4; Human Herpesvirus 8; IRF3 gene; Immune; Immune Evasion; Immune response; Immune signaling; Immunocompromised Host; Infection; Innate Immune Response; Interferon Suppression; Interferons; Knowledge; Laboratories; Ligase; Link; Lymphatic Endothelial Cells; Malignant Neoplasms; Mediating; Metabolic; Molecular; Molecular Target; Multienzyme Complexes; Mus; Natural Immunity; Oral; Oral cavity; Pathogenesis; Pathway interactions; Phosphotransferases; Post-Translational Protein Processing; Production; Proteins; Publishing; RNA; Recruitment Activity; Regulation; Reporting; Role; Signal Pathway; Signal Transduction; Testing; Therapeutic; Tumorigenicity; Vaccine Design; Viral; Viral Proteins; Virus Diseases; Virus Replication; Work; cellular targeting; cytokine; cytosolic receptor; deamidation; enzyme activity; gammaherpesvirus; immunoregulation; in vivo; insight; interest; keratinocyte; lymphoid neoplasm; novel; nucleotide metabolism; pathogen; receptor; transmission process; tumorigenic; ubiquitin mediated proteasome degradation; viral RNA; viral interferon regulatory factor-1; virus host interaction

Herpesviruses are ubiquitous in humans and they have been implicated in diverse malignancies. Human Kaposi's sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus are associated with tumors of lymphoid, epithelial, and endothelial origin in immuno-compromised patients. The oral cavity is a crucial compartment for gamma herpesvirus infection, particularly important for viral replication, transmission and pathogenesis. Employing human oral keratinocytes and lymphatic endothelial cells, we will delineate multiple viral immune evasion mechanisms enabled by regulated protein deamidation, the simplest post-translational modification of proteins. Host innate immunity is the first line of defense and herpesviruses have evolved an array of mechanisms to evade innate immune responses. Studying human KSHV and murine γHV68, we have uncovered a novel immune evasion mechanism enabled by a family of viral pseudoenzymes and a cellular metabolic glutamine amidotransferase. The viral pseudoenzymes interact with the cellular glutamine amidotransferase and alter its activity to deamidate both cellular and viral proteins, potently evading the interferon induction at multiple steps. Our published and unpublished findings collectively support the conclusion that deamidation is a key mechanism regulating innate immune responses and herpesviruses exploit this mechanism to benefit their infection. In this study, we will investigate how protein deamidation regulates a cytosolic receptor in sensing viral RNA (Aim 1) and the activity of a viral protein in antagonizing interferon induction (Aim 2). Furthermore, we will define the molecular action by which KSHV and γHV68 deploy vGAT proteins to regulate PFAS in selectively deamidating cellular and viral proteins to evade innate immune detection (Aim 3). Collectively, this study will elucidate a novel mechanism whereby protein deamidation regulates multiple steps of interferon induction, a key innate immune signaling cascade. Our work will establish more general regulatory roles of protein deamidation in fundamental biological processes, such as immune responses. Findings gleaned from this study will advance our understanding in host immune recognition and interferon induction, and potentially guide our future efforts in vaccine design and antiviral therapeutics to treat malignancies associated with human KSHV and EBV.

疱疹病毒在人类中普遍存在，并且它们与多种疾病有关。 恶性肿瘤。人类卡波西肉瘤相关疱疹病毒（KSHV）和EB病毒是 与免疫功能低下的淋巴样、上皮和内皮源性肿瘤相关 患者口腔是γ疱疹病毒感染的关键部位， 对病毒复制、传播和发病机制很重要。使用人口腔角质形成细胞 和淋巴管内皮细胞，我们将描绘多种病毒免疫逃避机制， 通过调节蛋白质脱酰胺，最简单的蛋白质翻译后修饰。 宿主的先天免疫是第一道防线，疱疹病毒已经进化出一系列的免疫系统。 逃避先天免疫反应的机制。通过研究人KSHV和鼠γ HV 68， 发现了一种新的免疫逃避机制，由一个病毒假酶家族和一个 细胞代谢谷氨酰胺转移酶。病毒假酶与细胞内的 谷氨酰胺转移酶，并改变其活性，使细胞和病毒蛋白脱酰胺， 避免了干扰素的多步诱导。我们已发表和未发表的研究结果 支持脱酰胺是调节先天免疫应答的关键机制的结论 疱疹病毒利用这一机制来促进其感染。在这项研究中，我们将调查 蛋白质脱酰胺作用如何调节胞质受体在感受病毒RNA（Aim 1）中的作用及其活性 病毒蛋白拮抗干扰素诱导（目的2）。此外，我们将定义 KSHV和γ HV 68部署vGAT蛋白以选择性调节PFAS的分子作用 使细胞和病毒蛋白脱酰胺以逃避先天免疫检测（Aim 3）。总的来说，这 研究将阐明一种新的机制，即蛋白质脱酰胺调节多个步骤， 干扰素诱导，一个关键的先天免疫信号级联。我们的工作将建立更普遍的 蛋白质脱酰胺在基本生物过程中的调节作用，如免疫 应答从这项研究中收集的发现将促进我们对宿主免疫的理解 识别和干扰素诱导，并可能指导我们未来的疫苗设计和 治疗与人KSHV和EBV相关的恶性肿瘤的抗病毒治疗剂。