Metabolic Interactions Supporting Effective TCE Bioremediation under Various Biog

描述（由申请人提供）：优化基于脱卤球菌的生物修复系统，以处理三氯乙烯（TCE）污染的超级基金场地，依赖于对复杂微生物相互作用的知识密集型理解，这些相互作用塑造了暴露于各种地球化学条件下的TCE脱氯微生物群落的结构和功能稳健性。以往的研究表明，不同的地球化学参数，如pH值降低或替代终端电子受体的存在下，可能会导致不完全脱氯。我们建议使用分子，生物化学和分析工具的组合，以评估可持续的和定义的多菌株微生物三氯乙烯脱氯财团在连续流恒化器的相互作用，研究社区的相互作用和生物地球化学条件对脱氯活性的影响。具体来说，我们建议分析社区水平的转录组学，细胞间代谢组学，和先进的随机矩阵理论网络分析，以了解地球化学应力的三氯乙烯脱氯财团的影响。该项目旨在提供对影响厌氧含脱卤球菌微生物群落并控制其TCE脱氯能力的网络化基因调控和代谢物交换的基本理解。在拟议的工作中，将研究各种与环境相关的地球化学应力，包括pH值、盐度和向系统引入潜在的竞争性电子受体（例如，硫酸根离子）。这些地球化学应力的影响将被监测和跟踪，通过交换的代谢产物，细胞过程和遗传调控之间发生的相互作用和相互依赖的人口脱氯财团。所产生的知识将提高设计和优化主动工程解决方案的能力，以减少与成功的地下水生物修复相关的时间和成本。本研究拟探讨的具体目标是：1.构建可持续的三氯乙烯脱氯微生物联合体，其中包含多个确定的微生物物种，这些微生物物种代表了强大的三氯乙烯脱氯群落中的基本功能，并在恒化器中建立其持续增长。2.确定生理变化，遗传调控的变化，并发生在三氯乙烯脱氯财团在响应的变化，如pH值，盐度，和替代电子受体的存在下，生物地球化学条件的细胞间代谢的变化。元转录组学和代谢组学分析将被用来获得一个系统级的了解复杂的地球化学应力的财团的反应。3.建立生态地球化学条件、微生物遗传调控和代谢相互作用之间的相关性，以阐明对各种生态地球化学条件作出反应的聚生体成员之间的网络相互作用。研究结果将用于调查刺激或增强战略，可以稳定功能的脱氯社区在不断变化的地球化学条件。

DESCRIPTION (provided by applicant): The optimization of Dehalococcoides-based bioremediation systems to treat trichloroethene (TCE)-contaminated Superfund sites relies upon knowledge-intensive understanding of complex microbial interactions that shape the structural and functional robustness of TCE-dechlorinating microbial communities exposed to a variety of geochemical conditions. Previous studies have shown that varied geochemical parameters, such as decreased pH or the presence of alternative terminal electron acceptors can result in incomplete dechlorination. We propose to use a combination of molecular, biochemical and analytical tools to evaluate interactions within sustainable and defined multi-strain microbial TCE- dechlorinating consortia in continuous-flow chemostats to study community interactions and the effects of biogeochemical conditions on dechlorination activity. Specifically, we propose to analyze community-level transcriptomics, intercellular metabolomics, and advanced Random Matrix Theory network analysis to understand the impact of geochemical stresses to the TCE-dechlorinating consortia. This project seeks to deliver a fundamental understanding of networked gene regulations and metabolite exchanges that impact anaerobic Dehalococcoides- containing microbial communities and control their TCE dechlorination capabilities. In the proposed work, a variety of environmentally-relevant geochemical stresses will be investigated including pH, salinity and the introduction of potential competitive electron acceptors to the system (e.g., sulfate ions). Effects of these geochemical stresses will be monitored and tracked through the exchanged metabolites, cellular processes and genetic regulations occurred amongst the interactive and interdependent populations in dechlorinating consortia. The generated knowledge will lead to an improved ability to design and optimize proactive engineering solutions to decrease the time and cost associated with successful groundwater bioremediation. The specific objectives to be investigated in this proposed study are, 1. Construct sustainable TCE-dechlorinating microbial consortia with multiple defined microbial species that represent the essential functions within robust TCE-dechlorinating communities, and establish their sustained growth in chemostats. 2. Identify the physiological changes, genetic regulatory changes, and the intercellular metabolic changes that occur in the TCE-dechlorinating consortia in response to changes in biogeochemical conditions, such as pH, salinity, and the presence of alternative electron acceptors. Metatranscriptomics and metabolomics analyses will be used to obtain a systems-level understanding of the complex responses of the consortia to geochemical stresses. 3. Establish correlations among biogeochemical conditions, microbial genetic regulations and metabolic interactions in order to elucidate the networked interactions among the members of the consortia that respond to various biogeochemical conditions. The results will be used to investigate stimulation or augmentation strategies that can stabilize the functions of dechlorination communities under changing geochemical conditions.