Metabolic Interactions Supporting Effective TCE Bioremediation under Various Biog

描述(由申请人提供)：基于脱卤球化合物的生物修复系统的优化，以处理三氯乙烯(TCE)污染的超级基金场地，依赖于对复杂的微生物相互作用的知识密集型理解，这些相互作用塑造了暴露在各种地球化学条件下的TCE脱氯微生物群落的结构和功能稳定性。以前的研究表明，不同的地化参数，如降低的pH值或替代的末端电子受体的存在，可能导致不完全的脱氯。我们建议使用分子、生化和分析工具的组合来评估连续流动恒化器中可持续的和已定义的多菌株微生物TCE-脱氯联合体之间的相互作用，以研究群落相互作用和生物地球化学条件对脱氯活性的影响。具体地说，我们建议分析社区水平的转录组学、细胞间代谢组学和高级随机矩阵理论网络分析，以了解地化压力对TCE脱氯联合体的影响。该项目旨在提供对影响厌氧脱卤球菌类微生物群落的联网基因调控和代谢物交换的基本了解，并控制它们的三氯乙烯脱氯能力。在拟议的工作中，将调查各种与环境有关的地球化学压力，包括pH、盐度和向该系统引入潜在的竞争性电子受体(例如，硫酸盐离子)。这些地球化学压力的影响将通过在脱氯联合体中相互作用和相互依赖的种群之间发生的交换代谢物、细胞过程和遗传调节来监测和跟踪。产生的知识将提高设计和优化主动工程解决方案的能力，以减少与成功的地下水生物修复相关的时间和成本。这项拟议研究的具体目标是：1.构建可持续的三氯乙烯脱氯微生物联合体，包括代表强大的三氯乙烯脱氯群落基本功能的多个定义的微生物种类，并建立它们在恒化器中的持续生长。2.确定TCE-脱氯联合体中发生的生理变化、基因调控变化和细胞间代谢变化，以响应生物地球化学条件的变化，如pH、盐度和替代电子受体的存在。将使用代谢组学和新陈代谢组学分析来获得对联合体对地球化学压力的复杂反应的系统水平的了解。3.建立生物地球化学条件、微生物遗传调控和代谢相互作用之间的关系，以阐明联合体成员之间对各种生物地球化学条件作出反应的网络相互作用。这些结果将被用于研究刺激或增强策略，这些策略可以在不断变化的地化条件下稳定脱氯群落的功能。

DESCRIPTION (provided by applicant): The optimization of Dehalococcoides-based bioremediation systems to treat trichloroethene (TCE)-contaminated Superfund sites relies upon knowledge-intensive understanding of complex microbial interactions that shape the structural and functional robustness of TCE-dechlorinating microbial communities exposed to a variety of geochemical conditions. Previous studies have shown that varied geochemical parameters, such as decreased pH or the presence of alternative terminal electron acceptors can result in incomplete dechlorination. We propose to use a combination of molecular, biochemical and analytical tools to evaluate interactions within sustainable and defined multi-strain microbial TCE- dechlorinating consortia in continuous-flow chemostats to study community interactions and the effects of biogeochemical conditions on dechlorination activity. Specifically, we propose to analyze community-level transcriptomics, intercellular metabolomics, and advanced Random Matrix Theory network analysis to understand the impact of geochemical stresses to the TCE-dechlorinating consortia. This project seeks to deliver a fundamental understanding of networked gene regulations and metabolite exchanges that impact anaerobic Dehalococcoides- containing microbial communities and control their TCE dechlorination capabilities. In the proposed work, a variety of environmentally-relevant geochemical stresses will be investigated including pH, salinity and the introduction of potential competitive electron acceptors to the system (e.g., sulfate ions). Effects of these geochemical stresses will be monitored and tracked through the exchanged metabolites, cellular processes and genetic regulations occurred amongst the interactive and interdependent populations in dechlorinating consortia. The generated knowledge will lead to an improved ability to design and optimize proactive engineering solutions to decrease the time and cost associated with successful groundwater bioremediation. The specific objectives to be investigated in this proposed study are, 1. Construct sustainable TCE-dechlorinating microbial consortia with multiple defined microbial species that represent the essential functions within robust TCE-dechlorinating communities, and establish their sustained growth in chemostats. 2. Identify the physiological changes, genetic regulatory changes, and the intercellular metabolic changes that occur in the TCE-dechlorinating consortia in response to changes in biogeochemical conditions, such as pH, salinity, and the presence of alternative electron acceptors. Metatranscriptomics and metabolomics analyses will be used to obtain a systems-level understanding of the complex responses of the consortia to geochemical stresses. 3. Establish correlations among biogeochemical conditions, microbial genetic regulations and metabolic interactions in order to elucidate the networked interactions among the members of the consortia that respond to various biogeochemical conditions. The results will be used to investigate stimulation or augmentation strategies that can stabilize the functions of dechlorination communities under changing geochemical conditions.