A fiber-coupled multimodal imaging platform for in vitro assessment of engineering tissue

用于工程组织体外评估的光纤耦合多模态成像平台

• 批准号: 9434895
• 负责人: Laura Marcu
• 金额: $ 7.85万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9434895
• 关键词: 3D Print; Address; Algorithms; American Heart Association; Area; Arteries; Autologous; Back; Biochemical; Biochemistry; Biocompatible Materials; Biological; Biological Assay; Biophotonics; Bioreactors; Blood Vessels; Bypass; Cardiovascular Diseases; Cause of Death; Cell Survival; Cells; Cessation of life; Computer software; Coronary heart disease; Coupled; Detection; Development; Disease; Drug or chemical Tissue Distribution; Endothelial Cells; Engineering; Ensure; Environment; Extracellular Matrix; Fiber; Fiber Optics; Fluorescence; Geometry; Goals; Green Fluorescent Proteins; Harvest; High Pressure Liquid Chromatography; Histology; Image; Image Analysis; In Vitro; Joints; Label; Laboratories; Light; Maps; Measurement; Measures; Medial; Monitor; Morphology; Motivation; Multi-modal optical imaging; Multimodal Imaging; Optical Coherence Tomography; Optics; Patients; Penetration; Performance; Perfusion; Prevalence; Process; Public Health; Publishing; Regenerative Medicine; Research; Research Personnel; Resolution; Rotation; Sampling; Sampling Errors; Scanning; Side; Smooth Muscle Myocytes; Spatial Distribution; Speed; Sterility; Structural Protein; Structure; Surface; System; Techniques; Testing; Thick; Time; Tissue Engineering; Tubular formation; Tunica Media; Validation; Work; attenuation; cost; crosslink; design; flexibility; fluorescence imaging; fluorescence lifetime imaging; image processing; imaging modality; imaging platform; imaging system; indexing; innovation; interest; lens; mechanical properties; monolayer; multimodality; novel; optical fiber; optical imaging; prototype; scaffold; statistics; tissue phantom; vascular tissue engineering

Project summary/Abstract Cardiovascular disease is a leading cause of death globally. In vitro engineering of replacement blood vessels has emerged as a promising area of research that has the potential to provide lifesaving therapy in patients where autologous bypass grafts are not possible. The challenges of vascular tissue engineering require the developing samples to be monitored at regular intervals, however conventional assessment techniques are slow, laborious and destructive. The goal of this proposal is to realize and validate a multimodal optical imaging platform that uses a single flexible fiber optic interface to acquire non-destructive measurements on-demand from the luminal surface of vascular constructs developing inside a bioreactor. The platform is designed such that three independent but complimentary imaging modalities will be able to operate in parallel, these include fluorescence lifetime imaging (FLIm) for monitoring changes in construct biochemistry; optical coherence tomography (OCT) for monitoring construct morphology and microstructure; and steady state fluorescence imaging (SSFI) to track the proliferation of green fluorescent protein labeled endothelial cells across the luminal surface. The specific aims of this proposal are as follows: (1) To realize a multimodal imaging platform compatible with non-destructive in vitro assessment of the luminal surface regions of vascular constructs inside a bioreactor; (2) To validate the performance of the multimodal imaging platform using tissue phantoms and engineered vascular constructs. The significance of this proposal is it presents an opportunity for a paradigm shift in the assessment of engineered tissue, where conventional destructive techniques are supplanted with faster, non- destructive ones. The innovation of this proposal is that it will allow FLIm, OCT and SSFI to operate in parallel, using the same double-clad fiber interface to guide light to and from the sample. The successful completion of this work has the potential for significant impact in the field of regenerative medicine where supplanting destructive measurements for non-destructive ones will lower costs and allow for faster prototyping of novel biomaterials.

项目摘要/摘要 心血管疾病是全球主要的死亡原因。替代血管的体外工程研究 已经成为一个很有希望的研究领域，有可能为患者提供挽救生命的疗法 不能进行自体搭桥的地方。血管组织工程的挑战要求 开发要定期监测的样品，然而传统的评估技术很慢， 费力且具有破坏性。该方案的目标是实现和验证多模式光学成像 使用单个灵活的光纤接口按需获取非破坏性测量的平台 从生物反应器内发育的血管结构的管腔表面。该平台是这样设计的 三种独立但互补的成像设备将能够并行运行，其中包括 用于监测结构生物化学变化的荧光寿命成像(FLIM)；光学相干 用于监测结构形态和微结构的层析成像(OCT)；以及稳态荧光 成像(SSFI)追踪绿色荧光蛋白标记的血管内皮细胞跨越管腔的增殖 浮出水面。该方案的具体目标如下：(1)实现多通道成像平台的兼容 对生物反应器内血管结构的管腔表面区进行非破坏性体外评估； (2)利用组织模体和工程化模型对多通道成像平台的性能进行验证 血管构造。这一提议的意义在于，它提供了一个转变 对工程组织的评估，其中传统的破坏性技术被更快的、非 具有破坏性的。这项提议的创新之处在于，它将允许FLIM、OCT和SSFI并行运行， 使用相同的双包层光纤接口将光引导到样品和从样品传出。圆满完成 这项工作有可能对再生医学领域产生重大影响， 对非破坏性测量的破坏性测量将降低成本，并允许更快地制作新的原型 生物材料。