Molecular Biology/Molecular Genetics (Core C)

MBMG核心 项目摘要/摘要 分子生物学/分子遗传学(MBMG)核心服务于NYORC肥胖研究社区 在过去的4个周期的这笔赠款。MBMG核心为肥胖相关临床提供关键研究支持 以及分子遗传学，生物信息学和模式生物领域的基础研究活动 人物刻画。这些服务得到了广泛的使用，提高了NORC调查人员的工作效率，同时 降低成本和便利获得先进的基因组学技术。 这个核心的主要目标是帮助调查人员应用分子生物学的工具和技术 遗传学和基因组学阐明发病和死亡的分子遗传学基础 肥胖症的医学/生理并存。MBMG核心的具体目标是：1)促进 通过为两项研究设计提供专家咨询，将分子遗传学应用于肥胖问题 和适用的分子生物学技术；2)为年轻人提供标准的实验室服务 研究人员和那些没有正式实验室进行肥胖相关研究的人；3)开发和提供 新的尖端研究工具和试剂。为此，MBMG Core提供了其他服务， 咨询和服务，包括基因表达、DNA提取、基因分型、DNA和RNA测序， 16S rRNA鉴定细菌系统发育，小鼠转基因，干细胞来源和 分化，以及使用CRISPR Cas9进行基因组编辑，这是与这些技术相关的分析工具。堆芯 服务有3种基本类型：(I)现场直接服务。(2)协商--与机构核心合作。 (3)协商--转介。为了获得尖端技术，避免重复，并最大限度地 利用我们的资源，我们与当地其他核心设施和实验室进行了广泛的合作。堆芯 人员提供从研究设计到研究执行和数据解释的各种协助。我们 特别强调为年轻的调查人员和P&F补助金持有者提供服务。核心一直活跃在 开发新的研究工具和试剂，并在建立iPS细胞方面发挥了至关重要的作用 为我们的社区提供技术。我们的研发活动专注于开发用于大脑的新工具和试剂 在细胞和模型动物中的研究和遗传操作。MBMG核心一直是 智力交流与合作，是学生、研究员和青年的重要培训场所 教职员工。在上一个周期中，核心响应了来自34个NORC用户的3,000个服务请求 49笔赠款，另外还有11名非兽人委员会成员。这些服务帮助调查人员产生了35个新的 赠款和147种出版物。

MBMG Core PROJECT SUMMARY/ABSTRACT The Molecular Biology/Molecular Genetics (MBMG) Core has served the NYORC obesity research community for the past 4 cycles of this grant. The MBMG Core provides critical research support for obesity-related clinical and basic research activities in the area of molecular genetics, bioinformatics, and model organism characterization. The services are widely used and have increased productivity of NORC investigators, while curtailing costs and facilitating access to advanced genomics technologies. The primary objective of this Core is to assist investigators to apply the tools and technologies of molecular genetics and genomics to elucidate the molecular-genetic bases for the pathogenesis and medical/physiological co-morbidities of obesity. The Specific Aims of the MBMG Core are: 1) To facilitate the application of molecular genetics to problems of obesity by providing expert consultation on both study design and applicable molecular biological techniques; 2) To provide standard laboratory services to young investigators and those without formal labs for studies related to obesity; 3) To develop and make available new cutting edge research tools and reagents. To these ends, the MBMG Core offers, among others, consultation and services, including gene expression, DNA extraction, genotyping, DNA and RNA sequencing, bacterial phylogenetic characterization by 16s rRNA, mouse transgenics, stem cell derivation and differentiation, and genome editing with CRISPR Cas9, analytic tools related to these techniques. Core services are of 3 basic types: (i) On-site Direct Services. (ii) Consultative-Collaborative with Institutional Cores. (iii) Consultative-Referral. To gain access to cutting-edge technologies, avoid duplication and to maximally leverage our resources, we collaborate extensively with other local Core facilities and laboratories. Core personnel provide assistance ranging from study design to execution of studies and data interpretation. We particularly emphasize service to young investigators and holders of P&F grants. The Core has been active in developing new research tools and reagents, and has played an essential role in establishing iPS cell technology for our community. Our R&D activities are focused on developing new tools and reagents for brain research and genetic manipulations in cells and model animals. The MBMG Core has been a nexus for intellectual exchange and collaboration, and an important training venue for students, fellows and young faculty. During the past cycle, the Core responded to >3,000 service requests from 34 NORC users supported by 49 grants, in addition to 11 non-ORC members. These services helped investigators generate 35 new grants and 147 publications.