Mouse Genome Engineering Core Facility

小鼠基因组工程核心设施

• 批准号: 9338261
• 负责人: Channabasavaiah Gurumurthy
• 金额: $ 4.2万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9338261
• 关键词: Animal Model; Biomedical Research; Client; Complex; Consultations; Core Facility; Development; Developmental Process; Disease; Embryo; Embryo Transfer; Equipment; Faculty; Female; Funding; Future; Generations; Genetic; Genetic Engineering; Genetically Engineered Mouse; Genome engineering; Genotype; Goals; Human Resources; Individual; Injection of therapeutic agent; Institutes; Investigation; Investments; Knockout Mice; Laboratories; Learning; Medical center; Methods; Microinjections; Micromanipulation; Molecular Biology; Mouse Strains; Mus; Nature; Nebraska; Nobel Prize; Operative Surgical Procedures; Phase; Procedures; Recombinants; Research; Research Infrastructure; Research Personnel; Resources; Services; Speed; Students; System; Techniques; Technology; Time; Training; Training and Education; Transgenes; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Universities; base; blastocyst; congenic; cost effective; design; embryonic stem cell; genome editing; improved; innovation; interest; member; model design; mouse genome; mouse model; neurosensory; new technology; pregnant; skills; success; training opportunity; transcription activator-like effector nucleases; vector

As one of the important scientific cores of the Center for the Molecular Biology of Neurosensory Systems, the goals of Mouse Genome Engineering Core facility (MGECF) are i) to serve all the needs of CoBRE investigators related to the design and creation of transgenic and knockout mouse models, ii) undertake technological research to continuously Improve genetic engineering methods and iii) to provide education and training opportunities to students, researchers and faculty. MGECF was supported by CoBRE during its Phase I at which time the facility was in the initial stages of development. By the end of Phase I the facility was fully functional in creating the mouse models by employing standard mouse embryo manipulation techniques such as microinjection and transferring into pseudo-pregnant females. During the CoBRE phase II period the facility has achieved significant accomplishments: i) started offering critical services such as the design and building of transgenic and targeting vectors that were done previously by the investigators; ii) initiated many other services such as mouse genotyping and speed congenics; iii) acquired additional equipment and personnel and provided training to enhance their overall skills (both scientific and administrative); and iv) initiated research on the most advanced genome engineering methods to validate them for offering as new services. Notably, the MGECF is among the first three such core facilities in the nation that started offering'the genome editing service TALENs (Transcription Activator Like Effector Nucleases). The core almost tripled its user base in 2010 and has maintained it at that level since then. It has has successfully increased its revenue in the recent three years, becoming less dependent of CoBRE support and demonstrating its capability to become sustainable in the future. This core facility is one of the most critical core facilities in the region that serves many investigators across three different institutes. Because of necessity as well as its remarkable success, the MGECF is supported by the UNMC office of the Vice Chancellor for Research. Additionally, the VCR office has a strong commitment to provide support beyond Phase III of CoBRE support.

作为神经感觉系统分子生物学中心的重要科学核心之一， 小鼠基因组工程核心设施(MGECF)的目标是：1)满足Cobre的所有需求 与转基因和基因敲除小鼠模型的设计和创建有关的调查人员，二)承担 不断改进基因工程方法的技术研究和三)提供教育 并为学生、研究人员和教职员工提供培训机会。MGECF在Cobre的支持下 第一阶段，当时该设施正处于发展的初始阶段。到第一阶段结束时，该设施 通过使用标准的小鼠胚胎操作来创建小鼠模型 显微注射和移植到假孕雌性体内等技术。在科布雷阶段 II期间，该设施取得了重大成就：i)开始提供关键服务，如 设计和构建以前由调查人员完成的转基因和靶向载体；二) 启动了许多其他服务，如小鼠基因分型和速度遗传；iii)获得了更多 设备和人员，并提供培训，以提高他们的综合技能(包括科学和 行政)；以及iv)启动对最先进的基因组工程方法的研究以验证 他们提供了新的服务。值得注意的是，MGECF是 开始提供基因组编辑服务TALENS(转录激活因子样效应器)的国家 核酸酶)。2010年，核心用户几乎增加了两倍，自那以来一直保持在这一水平。它 在最近三年中成功地增加了收入，变得不那么依赖Cobre 支持并展示其在未来变得可持续的能力。这一核心设施是 该地区最关键的核心设施，为三个不同机构的许多调查人员提供服务。 由于必要性以及其显著的成功，MGECF得到了联合国赔偿委员会办事处的支持。 负责研究的副校长。此外，录像机办公室有提供支持的坚定承诺 超越科布雷支持的第三阶段。