Mouse Genome Engineering Core Facility

小鼠基因组工程核心设施

基本信息

批准号：
8921229
负责人：
Channabasavaiah Gurumurthy
金额：
$ 4.2万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8921229
关键词：
Animal Model Biomedical Research Client Complex Consultations Core Facility Development Developmental Process Disease Embryo Embryo Transfer Equipment Faculty Female Funding Future Generations Genetic Genetic Engineering Genetically Engineered Mouse Genome engineering Genotype Goals Human Resources Individual Injection of therapeutic agent Institutes Investigation Investments Knockout Mice Laboratories Learning Medical center Methods Microinjections Micromanipulation Molecular Biology Mouse Strains Mus Nature Nebraska Nobel Prize Operative Surgical Procedures Phase Procedures Recombinants Research Research Personnel Resources Services Speed Staging Students System Techniques Technology Time Training Training and Education Transcription Coactivator Transgenes Transgenic Mice Transgenic Organisms United States National Institutes of Health Universities base blastocyst congenic cost effective design embryonic stem cell genome editing improved innovation interest meetings member model design mouse genome mouse model new technology nuclease pregnant skills success vector

项目摘要

As one of the important scientific cores of the Center for the Molecular Biology of Neurosensory Systems, the goals of Mouse Genome Engineering Core facility (MGECF) are i) to serve all the needs of CoBRE investigators related to the design and creation of transgenic and knockout mouse models, ii) undertake technological research to continuously Improve genetic engineering methods and iii) to provide education and training opportunities to students, researchers and faculty. MGECF was supported by CoBRE during its Phase I at which time the facility was in the initial stages of development. By the end of Phase I the facility was fully functional in creating the mouse models by employing standard mouse embryo manipulation techniques such as microinjection and transferring into pseudo-pregnant females. During the CoBRE phase II period the facility has achieved significant accomplishments: i) started offering critical services such as the design and building of transgenic and targeting vectors that were done previously by the investigators; ii) initiated many other services such as mouse genotyping and speed congenics; iii) acquired additional equipment and personnel and provided training to enhance their overall skills (both scientific and administrative); and iv) initiated research on the most advanced genome engineering methods to validate them for offering as new services. Notably, the MGECF is among the first three such core facilities in the nation that started offering'the genome editing service TALENs (Transcription Activator Like Effector Nucleases). The core almost tripled its user base in 2010 and has maintained it at that level since then. It has has successfully increased its revenue in the recent three years, becoming less dependent of CoBRE support and demonstrating its capability to become sustainable in the future. This core facility is one of the most critical core facilities in the region that serves many investigators across three different institutes. Because of necessity as well as its remarkable success, the MGECF is supported by the UNMC office of the Vice Chancellor for Research. Additionally, the VCR office has a strong commitment to provide support beyond Phase III of CoBRE support.

作为神经感觉系统分子生物学中心的重要科学核心之一，鼠标基因组工程核心设施（MGECF）的目标是i）满足鞋底的所有需求与转基因和淘汰小鼠模型的设计和创建有关的研究人员，ii）进行技术研究以不断改善基因工程方法和iii）提供教育以及向学生，研究人员和教职员工培训机会。 MGECF在其期间得到了Cobre的支持第一阶段，该设施处于开发的初始阶段。到第一阶段结束时，设施通过采用标准的鼠标胚胎操纵来创建鼠标模型，在创建鼠标模型方面充分发挥了作用诸如显微注射和转移到伪怀孕的女性之类的技术。在毛th期 ii期间该设施取得了重大成就：i）开始提供关键服务，例如研究人员先前完成的转基因和靶向向量的设计和建造； ii）启动了许多其他服务，例如小鼠基因分型和速度友善； iii）获得了更多设备和人员提供培训以提高其整体技能（科学和行政）; iv）对最先进的基因组工程方法进行了研究，以验证他们作为新服务提供。值得注意的是，MGECF是前三个这样的核心设施之一开始提供基因组编辑服务talens的国家（转录激活器，例如效应器核酸酶）。核心在2010年几乎将其用户群几乎增加了两倍，从那以后就将其维持在该级别。它最近三年已成功增加了收入支持并证明其未来具有可持续性的能力。这个核心设施是该地区最关键的核心设施为三个不同机构的许多调查人员提供服务。由于必要及其杰出成功，MGECF得到了UNMC办公室的支持研究副校长。此外，VCR办公室有坚定的承诺来提供支持超越了山科支撑的第三阶段。