Mouse Genome Engineering Core Facility

小鼠基因组工程核心设施

• 批准号: 8751149
• 负责人: Channabasavaiah Gurumurthy
• 金额: $ 4.2万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8751149
• 关键词: Animal Model; Biomedical Research; Client; Complex; Consultations; Core Facility; Development; Developmental Process; Disease; Embryo; Embryo Transfer; Equipment; Faculty; Female; Funding; Future; Generations; Genetic; Genetic Engineering; Genetically Engineered Mouse; Genome; Genome engineering; Genotype; Goals; Human Resources; Individual; Injection of therapeutic agent; Institutes; Investigation; Investments; Knockout Mice; Laboratories; Learning; Medical center; Methods; Microinjections; Micromanipulation; Molecular Biology; Mouse Strains; Mus; Nature; Nebraska; Nobel Prize; Operative Surgical Procedures; Phase; Procedures; Recombinants; Research; Research Personnel; Resources; Services; Speed; Staging; Students; System; Techniques; Technology; Time; Training; Training and Education; Transcription Coactivator; Transgenes; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Universities; base; blastocyst; congenic; cost effective; design; embryonic stem cell; improved; innovation; interest; meetings; member; model design; mouse genome; mouse model; new technology; nuclease; pregnant; skills; success; vector

As one of the important scientific cores of the Center for the Molecular Biology of Neurosensory Systems, the goals of Mouse Genome Engineering Core facility (MGECF) are i) to serve all the needs of CoBRE investigators related to the design and creation of transgenic and knockout mouse models, ii) undertake technological research to continuously Improve genetic engineering methods and iii) to provide education and training opportunities to students, researchers and faculty. MGECF was supported by CoBRE during its Phase I at which time the facility was in the initial stages of development. By the end of Phase I the facility was fully functional in creating the mouse models by employing standard mouse embryo manipulation techniques such as microinjection and transferring into pseudo-pregnant females. During the CoBRE phase II period the facility has achieved significant accomplishments: i) started offering critical services such as the design and building of transgenic and targeting vectors that were done previously by the investigators; ii) initiated many other services such as mouse genotyping and speed congenics; iii) acquired additional equipment and personnel and provided training to enhance their overall skills (both scientific and administrative); and iv) initiated research on the most advanced genome engineering methods to validate them for offering as new services. Notably, the MGECF is among the first three such core facilities in the nation that started offering'the genome editing service TALENs (Transcription Activator Like Effector Nucleases). The core almost tripled its user base in 2010 and has maintained it at that level since then. It has has successfully increased its revenue in the recent three years, becoming less dependent of CoBRE support and demonstrating its capability to become sustainable in the future. This core facility is one of the most critical core facilities in the region that serves many investigators across three different institutes. Because of necessity as well as its remarkable success, the MGECF is supported by the UNMC office of the Vice Chancellor for Research. Additionally, the VCR office has a strong commitment to provide support beyond Phase III of CoBRE support.

作为神经感觉系统分子生物学中心的重要科学核心之一， 小鼠基因组工程核心设施（MGECF）的目标是：i）满足CoBRE的所有需求 与转基因和基因敲除小鼠模型的设计和创建相关的研究人员，ii）承担 技术研究，以不断改进基因工程方法; iii）提供教育 和培训机会的学生，研究人员和教师。MGECF得到了CoBRE的支持， 第一阶段，当时该设施正处于发展的初始阶段。到第一阶段结束时， 通过使用标准的小鼠胚胎操作， 技术，如显微注射和转移到假怀孕的女性。在CoBRE阶段 在第二阶段，该设施取得了重大成就：i）开始提供关键服务， 设计和构建转基因载体和靶向载体，这是研究人员以前完成的; ii） 启动了许多其他服务，如小鼠基因分型和速度同源性; iii）获得了额外的 设备和人员，并提供培训，以提高他们的整体技能（科学和 （四）启动最先进的基因组工程方法研究， 作为新的服务提供。值得注意的是，该基金是世界上最早的三个核心设施之一。 一个国家开始提供“基因组编辑服务TALENs（转录激活因子样效应器 核酸酶）。核心在2010年几乎将其用户基础增加了两倍，并从那时起一直保持在这一水平。它 在最近三年中，它成功地增加了收入，减少了对Cobre的依赖 支持并展示其未来可持续发展的能力。这个核心设施是 该区域最重要的核心设施，为三个不同研究所的许多研究人员提供服务。 由于必要性以及其显著的成功，MGECF得到了联合国MC办公室的支持， 副校长研究。此外，VCR办公室有一个强有力的承诺，提供支持 第三阶段的CoBRE支持。