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Functions of distinct trigeminal brainstem projection pathways in tactile behavior

不同三叉神经脑干投射通路在触觉行为中的功能

基本信息

批准号：
9298006
负责人：
Fan Wang
金额：
$ 21.75万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-03-01 至 2019-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9298006
关键词：
Address Animals Applications Grants Area Behavior Behavioral Brain Brain Stem Cannulas Cations Cell Nucleus Cells Chloride Channels Chronic Code Complex Detection Discrimination Dissection Electrophysiology (science)Emotions Environment Esthesia Face Fiber Optics Future Genetic Goals Impairment Individual Injury Lead Learning Lentivirus Vector Light Medial Modality Motor Movement Mus Neurons Outcome Output Pathologic Pathway interactions Perception Physiological Population Posterior Thalamic Nuclei Preparation Process Property Proteins Research Resolution Rodent Role Rosa Sensory Signal Transduction Site Social Interaction Source Specificity Speed Spinal Stimulus Stream Structure of trigeminal ganglion System Tactile Techniques Technology Tetanus Toxin Texture Thalamic structure Touch sensation Training Translating Trigeminal System Vibrissae Viral Viral Vector awake base behavior measurement experience experimental study extracellular follow-up high risk implantation in vivo innovation insight multi-electrode arrays neural circuit neuromechanism novel optogenetics receptor relating to nervous system response sensory discrimination somatosensory spatiotemporal technological innovation temporal measurement

项目摘要

Tactile sensation is paramount to how we experience the physical world around us. Species like mice use their facial whiskers to navigate and assess their environment. Although there is a basic understanding of how brainstem trigeminal neurons, the first central relay for whisker-derived signals, respond to passive tactile stimuli, little is known about their activities and functions during active behaviors. Tactile exploration is an active sensing process, which adapts to behavioral purposes, and responses of trigeminal brainstem neurons are likely adjusted accordingly. Furthermore, brainstem projection neurons contribute to two main ascending pathways, lemniscal and paralemniscal, but the functions of these two distinct pathways remain unclear. Our goal for this high-risk exploratory grant application is to develop a chronic in vivo recording preparation, perform recordings from optogenetically identified lemniscal or paralemniscal projection neurons during active behaviors, and use causal manipulations to determine their functions. Recording and manipulating pathway-specific neurons in vivo, and making accurate behavioral measurements in freely moving animals are both challenging. We will use a combination of technological innovations to overcome these challenges. The outcome of this project will establish a platform for future in-depth dissection of how touch sensory information is integrated, transformed and delivered to different processing streams by trigeminal brainstem to generate perception, invoke emotion and/or inform decisions.

触觉对于我们如何体验周围的物理世界至关重要。像老鼠这样的物种它们的胡须来导航和评估环境。虽然有一个基本的理解，脑干三叉神经元是第一个传递胡须信号的中枢神经元，触觉刺激，很少有人知道他们的活动和功能，在主动行为。触觉探索是主动感知过程，适应行为目的，三叉神经脑干反应神经元可能会相应地调整。此外，脑干投射神经元有助于两个主要的功能，上升途径，丘系和腹系，但这两个不同的途径的功能仍然存在不清楚我们的目标是这个高风险的探索性拨款申请是开发一种慢性体内记录制备，从光遗传学鉴定的丘系或丘系投射神经元进行记录在积极的行为，并使用因果操纵，以确定其功能。记录和在体内操纵通路特异性神经元，并在自由环境中进行精确的行为测量。移动动物都是挑战。我们将利用技术创新的组合来克服这些挑战。该项目的成果将为未来深入剖析如何建立一个平台触摸感觉信息被集成、转换并传送到不同的处理流，三叉神经脑干产生知觉，唤起情感和/或通知决策。