Defining Brainstem Neuronal Pathways for Orofacial Motor Actions

定义口面部运动动作的脑干神经元通路

• 批准号: 10413914
• 负责人: Fan Wang
• 金额: $ 51.1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10413914
• 关键词: Action Research; Address; Adult; Anatomy; Architecture; Atlases; Automobile Driving; Behavior; Brain Stem; Breathing; Cells; Data; Discrimination; Electrophysiology (science); Exploratory Behavior; Genetic; Goals; Head; Individual; Jaw; Label; Larynx; Lateral; Lead; Maps; Mastication; Methods; Modeling; Molecular; Motor; Motor Neurons; Movement; Mus; Muscle; Nature; Neonatal; Neurons; Neurotransmitters; Nose; Pathway interactions; Periodicity; Phenotype; Population Heterogeneity; Positioning Attribute; Presynaptic Terminals; Property; Rabies virus; Research Project Grants; Reverse engineering; Structure; Technology; Testing; Texture; Tongue; Training; Vibrissae; Viral; Work; base; design; digital; feeding; genetic approach; insight; interest; mature animal; motor control; neonatal mice; neonate; operation; orofacial; preBotzinger complex; theories; tool; virus genetics; vocalization

Project 1. Abstract Defining brainstem neuronal pathways for orofacial motor actions This Research Project will define the neuronal phenotypes and their connectivity, i.e., the “Components" and "Wiring diagrams", for premotor and higher order, e.g., pre2motor pathways for rhythmic orofacial motor actions. This will reveal the control structure for coordinating motor actions. Primary technologies for this work are transsynaptic viral tracing in both neonates and adult mice, intersectional labeling strategies followed by transsynaptic tracing in adult animals, and intersectional strategies to manipulate neuronal activity in order to infer function. Adult data will be added to the Trainable Texture-based Digital Atlas (Project 5 and Core 2) These data will yield essential circuitry for orofacial exploratory actions. Our five-year horizon is anatomical identification of pre- and pre2motor pathways for key orofacial senses: twitching and directional movement of by the nose, shifts in the position of the mystacial pad driving set point changes, and completion of the circuit for whisking, bobbing of the head. We further consider anatomical circuitry underlying motor control of the tongue for motor actions involved in search or discrimination tasks, along with vocalization. These data provide a set of connections that constrain models for the activation and operation of orofacial exploratory circuitry. They give insight into the operational tradeoffs that nature has made in network design. These data, along with complementary anatomy and electrophysiology data from Research Projects 2 and 3, provide input for a theoretical analyses of the control mechanisms that coordinate different orofacial actions during exploration (Project 4).

项目1。摘要 定义口腔运动动作的脑干神经元途径 该研究项目将定义神经元表型及其连接性，即“组件” 和“接线图”，用于前运动和更高阶的，例如，有节奏的Orofacial Motor的Pre2 Motor途径 动作。这将揭示协调电机动作的控制结构。这项工作的主要技术 是在新生儿和成年小鼠中跨突触病毒追踪，交叉标记策略，然后是 成年动物中的透射性追踪，以及操纵神经元活动的交叉策略 推断功能。成人数据将添加到可训练的基于纹理的数字地图集（项目5和Core 2） 这些数据将产生针对口面探索性作用的必需电路。我们的五年视野是解剖学的 识别主要口面感觉的前和前2MOTOR途径：抽搐和方向运动 在鼻子上，神秘垫驱动点的位置变化和电路的完成 为了搅拌，头部摇摆。我们进一步考虑了该解剖电路的基础电机控制 涉及搜索或歧视任务的运动动作的舌头以及发声。 这些数据提供了一组连接，这些连接将模型限制在口面上的激活和操作 探索性电路。他们深入了解自然在网络设计中所做的运营权衡。 这些数据，以及研究项目2和3的完整解剖学和电生理数据， 提供对协调不同种族作用的控制机制的理论分析的输入 在探索期间（项目4）。