Pre-motor Neural Circuits for Exploratory Movement

用于探索性运动的前运动神经回路

基本信息

批准号：
10348340
负责人：
Fan Wang
金额：
$ 32.85万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-02-15 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10348340
关键词：
Pre motor Neural Circuits Exploratory

项目摘要

ABSTRACT/PROJECT SUMMARY Movements performed by animals in order to explore external objects are called exploratory movements. The rodent whisker-mediated tactile exploration is a prominent model of exploratory movements. It is known that fast sensory feedback, attention, memory and other factors modulate the patterns of whisker movements in different behavioral contexts. However, the precise neural circuits mechanisms that mediate such task- or context-dependent control of whisker movement patterns remain largely unknown. Through our monosynaptic whisker premotor circuit mapping studies and on-going functional characterizations, we have now identified a group of premotor neurons mediating a sensory feedback reflex, and also discovered the putative premotor whisking oscillator (rhythmogenic) neurons. The goal of this competitive renewal is to determine the in vivo activity patterns of these identified premotor neurons (sensory feedback and the rhythmogenic premotor neurons) in different behavior contexts, mapping their presynaptic inputs (i.e. identifying pre-premotor neurons, or pre2motor neurons) and begin to unveil how key pre2motor neurons convey context-dependent modulation of whisker movements. We will use a combination of viral genetic intersectional labeling strategy, in vivo optetrode-array based recording of photo-identified premotor neurons, modeling, non-toxic viral mediated transsynaptic labeling and manipulation of pre2motor neurons in multiple behavioral tasks to achieve our goal.

摘要/项目摘要动物为探索外部物体进行的动作称为探索性运动。这啮齿动物晶须介导的触觉探索是探索性运动的重要模型。众所周知快速的感觉反馈，注意力，记忆和其他因素调节晶须运动的模式不同的行为环境。但是，介导此任务或晶须运动模式的上下文依赖性控制仍然很大程度上未知。通过我们的单突触晶须前电路映射研究和持续的功能表征，我们现在已经确定一组介导感官反馈反射的前前神经元，还发现了推定的先前搅拌振荡器（节奏）神经元。这种竞争性更新的目标是确定体内这些确定的前神经元的活动模式（感觉反馈和节律性前体神经元）在不同的行为环境中，绘制其突触前输入（即识别前部门神经元或前2MOTOR神经元）并开始揭示关键前2MOTOR神经元如何传达上下文依赖性的神经元晶须运动的调节。我们将结合病毒遗传交叉标记策略的组合，基于光学识别的前神经元的基于体内选词阵列的记录，建模，无毒病毒在多个行为任务中介导的透射性标记和操纵前2MOTOR神经元以实现我们的目标。