Pre-motor Neural Circuits for Exploratory Movement

用于探索性运动的前运动神经回路

• 批准号: 10204126
• 负责人: Fan Wang
• 金额: $ 34.82万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-15 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10204126
• 关键词: Agnosia; Anatomy; Animals; Attention; Back; Behavior; Behavioral; Development; Environment; Event; Face; Fingers; Funding; Genetic; Goals; Human; Intention; Knowledge; Label; Maps; Mediating; Memory; Methods; Modeling; Modernization; Molecular; Motor; Motor Neurons; Movement; Movement Disorders; Mus; Neurons; Organ; Parvalbumins; Patients; Pattern; Periodicity; Play; Progress Reports; Pump; Rabies virus; Rattus; Reflex action; Reflex control; Reticular Formation; Rodent; Role; Shapes; Structure of trigeminal nerve spinal tract nucleus; Tactile; Techniques; Testing; Texture; Touch sensation; Vibrissae; Viral; base; design; extracellular; genetic approach; in vivo; motor control; motor disorder; neural circuit; neuromechanism; neuroprosthesis; nucleus ambiguus; optogenetics; physical property; presynaptic; response; sensory feedback; sensory input; social; virus genetics

ABSTRACT/PROJECT SUMMARY Movements performed by animals in order to explore external objects are called exploratory movements. The rodent whisker-mediated tactile exploration is a prominent model of exploratory movements. It is known that fast sensory feedback, attention, memory and other factors modulate the patterns of whisker movements in different behavioral contexts. However, the precise neural circuits mechanisms that mediate such task- or context-dependent control of whisker movement patterns remain largely unknown. Through our monosynaptic whisker premotor circuit mapping studies and on-going functional characterizations, we have now identified a group of premotor neurons mediating a sensory feedback reflex, and also discovered the putative premotor whisking oscillator (rhythmogenic) neurons. The goal of this competitive renewal is to determine the in vivo activity patterns of these identified premotor neurons (sensory feedback and the rhythmogenic premotor neurons) in different behavior contexts, mapping their presynaptic inputs (i.e. identifying pre-premotor neurons, or pre2motor neurons) and begin to unveil how key pre2motor neurons convey context-dependent modulation of whisker movements. We will use a combination of viral genetic intersectional labeling strategy, in vivo optetrode-array based recording of photo-identified premotor neurons, modeling, non-toxic viral mediated transsynaptic labeling and manipulation of pre2motor neurons in multiple behavioral tasks to achieve our goal.

摘要/项目摘要 动物为探索外部物体进行的动作称为探索性运动。这 啮齿动物晶须介导的触觉探索是探索性运动的重要模型。众所周知 快速的感觉反馈，注意力，记忆和其他因素调节晶须运动的模式 不同的行为环境。但是，介导此任务或 晶须运动模式的上下文依赖性控制仍然很大程度上未知。通过我们的单突触 晶须前电路映射研究和持续的功能表征，我们现在已经确定 一组介导感官反馈反射的前前神经元，还发现了推定的先前 搅拌振荡器（节奏）神经元。这种竞争性更新的目标是确定体内 这些确定的前神经元的活动模式（感觉反馈和节律性前体 神经元）在不同的行为环境中，绘制其突触前输入（即识别前部门 神经元或前2MOTOR神经元）并开始揭示关键前2MOTOR神经元如何传达上下文依赖性的神经元 晶须运动的调节。我们将结合病毒遗传交叉标记策略的组合， 基于光学识别的前神经元的基于体内选词阵列的记录，建模，无毒病毒 在多个行为任务中介导的透射性标记和操纵前2MOTOR神经元以实现 我们的目标。