Microscopic Imaging and Analysis

显微成像和分析

• 批准号: 9234417
• 负责人: Anastas Popratiloff
• 金额: $ 16.04万
• 依托单位: GEORGE WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9234417
• 关键词: 22q11 Deletion Syndrome; Adherence; Child; Childhood; Competence; Consult; Cost Savings; Cranial Nerves; Data; Data Analyses; Data Collection; Data Quality; Deglutition Disorders; Development; Embryo; Ensure; Equilibrium; Equipment; Gene Expression; Generations; Genetic Models; Goals; Guidelines; Health system; Human Resources; Image; Image Analysis; Imagery; In Situ Hybridization; Information Technology; Laboratories; Libraries; Mental Retardation and Developmental Disabilities Research Centers; Methodology; Mission; Molecular; Morphogenesis; Morphology; Mus; National Institute of Neurological Disorders and Stroke; Neurodevelopmental Disorder; Neurons; Optics; Pathogenicity; Pathology; Performance; Phenotype; Phototoxicity; Prevention; Protocols documentation; Publications; Quality Control; Reporting; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Scientist; Supervision; System; Technology; Three-Dimensional Image; Training; Update; base; cellular imaging; design; embryo tissue; equipment acquisition; experimental study; image archival system; imaging approach; imaging modality; instrument; microscopic imaging; novel strategies; orofacial; outreach; personalized approach; pre-clinical; pre-clinical research; programs; temporal measurement; tissue preparation

ABSTRACT The PROJECTS described in this Program will examine the phenotypic effects of disrupted oro-facial and cranial nerve development and function in several mouse genetic models of 22q11 Deletion Syndrome. CORE D, Microscopic Imaging and Analysis, is designed to facilitate these PROJECTS by providing unique instruments, platforms for analysis, technical support, and extensive expertise for cellular, immuno- histochemical, and in situ hybridization analyses related to the Program's mission. In an era when no single laboratory can develop the highest level of technical competence in multiple important and highly specialized image analysis approaches, CORE D will tailor approaches to maximize the use and availability of state-of-art technology. CORE D will ensure that the imaging resources for the Program are maintained and constantly updated to achieve optimal performance. CORE D will be primarily responsible for refining existing approaches and designing new approaches for microscopic imaging. The Core personnel will advise Program investigators on adherence to preclinical research rigor guidelines. CORE D will provide information technology infrastructure and guidelines for maintaining a library of imaging data from all of the PROJECTS that will be accessible, via a password protected system, by all Program personnel. CORE D will utilize electronic media and seminars to disseminate research progress and to provide outreach opportunities in microscopic imaging methodology. The Core Director and the research scientist will be engaged in the design and implementation of microscopic imaging approaches and will consult with the PROJECT PIs, Co-Is and their staff from the design of the experiments to the reporting and publication of the results. Finally, CORE D will be instrumental in bridging the activities associated with the Program and those of the Cellular Imaging Core of the Intellectual and Developmental Disabilities Research Center (IDDRC) at Children's National Health System (CNHS). NARRATIVE CORE D, Microscopic Imaging and Analysis, will support Program investigators' analyses of changes in gene expression, morphogenesis, circuit development and function in mouse genetic models of 22q11 Deletion Syndrome. The Core will offer cutting edge technology and methodology unavailable in PROJECT PIs' laboratories to define pathogenic mechanisms in this neurodevelopmental disorder. The Core will support data analysis, and build an image archive accessible to all PROJECT investigators to facilitate integration and maximize resources between the three PROJECTS in this Program.

摘要 本计划中描述的项目将检查口腔面部和面部破裂的表型影响 22q11缺失综合征小鼠几种遗传模型的脑神经发育和功能。堆芯 D，显微成像和分析，旨在通过提供独特的 仪器、分析平台、技术支持以及细胞、免疫和免疫方面的广泛专业知识 组织化学和原位杂交分析与该计划的任务有关。在一个没有单身人士的时代 实验室可以在多个重要和高度专业化的领域发展最高水平的技术能力 图像分析方法，核心D将量身定做方法，以最大限度地使用和获得最先进的 技术核心D将确保计划的成像资源得到维护和持续 已更新以实现最佳性能。核心D将主要负责改进现有方法 以及设计新的显微成像方法。核心人员将为项目调查人员提供建议 关于坚持临床前研究的严谨指南。核心D将提供信息技术 用于维护来自所有项目的成像数据库的基础设施和指导方针 所有计划人员均可通过受密码保护的系统访问。核心D将利用电子媒体 和研讨会，以传播研究进展并提供显微成像方面的外展机会 方法论。核心主任和研究科学家将参与设计和实施 显微成像方法，并将与项目PIS、COIS及其工作人员协商 从实验设计到结果的报告和发布。最后，核心D将发挥重要作用 在与该计划相关的活动和智力的细胞成像核心的活动之间架起桥梁 和儿童国家卫生系统(CNHS)的发育障碍研究中心(IDDRC)。 叙事 核心D，显微成像和分析，将支持计划研究人员对基因变化的分析 22q11缺失小鼠遗传模型的表达、形态发生、回路发育及功能 综合症。核心将提供项目绩效指标所不具备的尖端技术和方法 实验室来确定这种神经发育障碍的致病机制。核心将支持数据 分析，并建立一个所有项目调查人员都可以访问的图像档案，以促进集成和 最大限度地利用本计划中三个项目之间的资源。