Zeiss 980 confocal microscope with 32-channel spectral detector, Airy Scan, and Chameleon Discovery IR laser for 2-photon excitation

Zeiss 980 共焦显微镜，配备 32 通道光谱检测器、Airy Scan 和 Chameleon Discovery IR 激光器，用于 2 光子激发

• 批准号: 10431118
• 负责人: Anastas Popratiloff
• 金额: $ 94.72万
• 依托单位: GEORGE WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-20 至 2023-07-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431118
• 关键词: Address; Advisory Committees; Area; Cells; Collaborations; Communities; Complex; Computers; Educational workshop; Electron Microscopy; Embryo; Fluorescence; Functional Imaging; Funding; Generations; Glutaral; Growth; Health Sciences; Image; Image Enhancement; Label; Lasers; Light; Lipids; Logistics; Microscope; Minor; Multimodal Imaging; NADH; Optics; Organ; Organism; Physiologic pulse; Research; Research Personnel; Resolution; Sampling; Scanning; Services; Source; System; Technology; Tissues; Training; Uncertainty; United States National Institutes of Health; Universities; base; biomedical resource; computerized data processing; confocal imaging; data format; detector; expectation; experimental study; imaging facilities; innovation; instrument; live cell imaging; medical schools; microscopic imaging; nanofabrication; nanoimaging; operation; photomultiplier; programs; quality assurance; second harmonic; two-photon

We request funds to purchase a Zeiss 980 confocal microscope equipped with a 32-channel linear array GaAsP photomultiplier spectral detector, Airyscan detector for high-resolution confocal imaging, visible laser launch, and Coherent Discovery (670-1330 nm) pulsed coherent light source for 2-photon excitation. The system will be equipped with an environmental chamber supporting live-cell experiments. The instrument will be programable to execute complex experimental sequences, capable of remote operations, collaborations, and multimodal image integration and will become a central piece of cross-technology workflows. The inherent ability of this instrument to generate large-format data will be addressed by two dedicated computers, synchronously managing the acquisition and processing of the data. The selected instrument will sustain and expand our current biomedical microimaging program following service termination of our workhorse spectral confocal – Zeiss 710. The system will serve a multitude of experiments, including live-cell imaging, photomanipulation, high-resolution large-area registrations, and large-volume registrations. The spectral capabilities of our Zeiss 710 have supported our research base to establish a robust biomedical microimaging program by eliminating uncertainties with fluorescence quality assurance, enabling imaging of cleared tissues and organisms presenting autofluorescence and tissues fixed with glutaraldehyde for correlative light-to-electron microscopy imaging. The proposed Zeiss 980 will empower us to continue in this direction while expanding our experimental portfolio offering also sub-diffraction microimaging - the standards of the next decade. Zeiss 980 will open doors for a more robust subcellular imaging, enhancing experiments focused on living and fixed cells and organisms, allowing discrete photomanipulation with visible and 2-photon excitation, functional imaging of NADH, label-free imaging of lipids, and second harmonic generation. The Airyscan will establish our portfolio in sub-diffraction resolution imaging of complex tissues, organs, and embryos, including optically cleared tissues and organs. This system will be exclusively available to the NIH-funded research community, with the major/minor users having access immediately after installation, but with the expectation to grow a user base, including newly NIH-funded investigators. This growth will follow a democratic principle involving the advisory committee. The well- established GW Nanofabrication and Imaging Center (GWNIC), which is the major technology resource for biomedical micro-and nanoimaging in the university, is supported by the GW’ Office of the Vice Provost for Research. GWNIC will provide administrative support, training of new users and will develop innovative approaches. Zeiss 980 will be a central component of cross-technology workflows such as correlative light- electron microscopy and will support our annual workshop. The instrument will be installed in GWNIC’s space within the School of Medicine and Health Sciences with the aim to effectively address the sample logistics and compliance by the biomedical segment of our research base.

我们申请资金购买配备32通道线性阵列的蔡司980共聚焦显微镜 GaAsP光电倍增管光谱探测器，用于高分辨率共焦成像的Airyscan探测器，可见激光 发射和相干发现（670-1330 nm）脉冲相干光源，用于双光子激发。系统 将配备一个支持活细胞实验的环境室。仪器将 可编程执行复杂的实验序列，能够远程操作，协作， 多模态图像集成，并将成为跨技术工作流程的核心部分。的固有能力 这台仪器产生大格式的数据将由两台专用计算机同步处理 管理数据的获取和处理。选定的仪器将维持和扩大我们目前的 生物医学显微成像计划后，我们的主力服务终止光谱共焦-蔡司710。 该系统将用于多种实验，包括活细胞成像，光操纵，高分辨率 大面积注册和大批量注册。蔡司710的光谱性能 通过消除不确定性，支持我们的研究基地建立一个强大的生物医学显微成像计划 具有荧光质量保证，能够对已清除的组织和生物体进行成像， 自体荧光和用戊二醛固定的组织用于相关的光-电子显微镜成像。的 建议蔡司980将使我们能够继续在这一方向，同时扩大我们的实验产品组合 还提供亚衍射显微成像-下一个十年的标准。蔡司980将为 更强大的亚细胞成像，增强实验重点是活的和固定的细胞和生物体， 允许用可见光和2-光子激发的离散光操纵，NADH的功能成像，无标记 脂质成像和二次谐波产生。Airyscan将建立我们的亚衍射产品组合 复杂组织、器官和胚胎的分辨率成像，包括光学透明的组织和器官。这 该系统将专门提供给NIH资助的研究社区，主要/次要用户有 安装后立即访问，但期望增加用户群，包括新的NIH资助的 investigators.这一增长将遵循涉及咨询委员会的民主原则。井- 建立了GW纳米纤维和成像中心（GWNIC），这是主要的技术资源， 生物医学显微和纳米成像在大学，是由GW的副教务长办公室的支持， Research. GWNIC将提供行政支持，培训新用户，并将开发创新的 接近。蔡司980将成为跨技术工作流程的核心组成部分，例如相关光- 电子显微镜，并将支持我们的年度研讨会。该仪器将安装在GWNIC的空间 在医学和健康科学学院，旨在有效地解决样品物流和 我们的研究基地的生物医学部分遵守。