Acquisition of Zeiss Cell Observer Spinning Disk Confocal System

收购 Zeiss Cell Observer 转盘共焦系统

• 批准号: 8247358
• 负责人: Anastas Popratiloff
• 金额: $ 49.95万
• 依托单位: GEORGE WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-15 至 2013-11-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247358
• 关键词: Acquired Immunodeficiency Syndrome; Address; Advisory Committees; Biological Assay; Cell Cycle; Cells; Child; Communities; Detection; Development; Developmental Disabilities; District of Columbia; Embryo; Engineering; Fluorescence; Funding; Future; Generations; HIV; Helminths; Hookworms; Image; Image Analysis; Institution; Intellectual functioning disability; Life; Light; Liver Fibrosis; Liver diseases; Maintenance; Medical center; Methodology; Microscope; Microscopic; Microscopy; Molecular; Mus; Nematoda; Organelles; Parasites; Parasitology; Phototoxicity; Principal Investigator; Radiation; Research; Research Infrastructure; Research Personnel; Sampling; Scanning; Signal Pathway; Signal Transduction; Specimen; System; Technology; Time; Training; Training Programs; Transgenic Organisms; United States National Institutes of Health; Universities; Washington; base; cell motility; cellular imaging; design; instrument; member; neoplastic cell; neurodevelopment; new technology; pathogen; programs; research study; trafficking

DESCRIPTION (provided by applicant): We propose to acquire the Carl Zeiss Cell Observer spinning disk confocal system, to support a large cadre of NIH-funded investigators at the George Washington University (GW) and its partner institutions. This system will primarily be dedicated to short and long-term live-cell imaging experiments, as well as other current applications, including high-throughput/high-content image acquisition. The acquisition of the instrument will surmount the large array of current barriers for live-cell imaging, a result of our existing point scanning confocal microscopes. For cutting-edge, live-cell imaging microscopy, these are essential technological requirements that must be addressed for time-lapse recording of fluorescence signals to study cell motility, organelle trafficking, free living nematodes (c. elegans) and murine genetically engineered embryos. The proposed spinning disk microscopic technology is uniquely tailored to rapid sampling of fluorescent images from living specimens, while providing high detection sensitivity and limited light radiation. Minimizing light radiation s critical for most live-cell functional assays in order to reduce potential phototoxicity and generation of reactive oxidative species. Spinning disk microscopes subject specimens to much lower light levels than the presently used point scan confocal microscopes and therefore ideally suited for live-cell imaging. Numerous projects will benefit from acquiring the instrument, including research in molecular mechanisms of neural development at the cellular and organelle levels, dysregulation of cell cycles associated with tumor cells, molecular parasitology investigating signaling pathways controlling re-initiation of hookworm development, host-pathogen interactions related to HIV and transgenic helminth parasites, and hepatobiology and molecular basis of liver diseases and fibrosis. The instrument will be integrated into the infrastructure of GW's well-established Center for Microscopy and Image Analysis (CMIA), and made available to members of NIH-funded Centers, including the Intellectual and Developmental Disabilities Research Center (IDDRC) at the GW-affiliated Children's National Medical Center and the District of Columbia Developmental Center for AIDS Research (DC D-CFAR). In addition, CMIA will manage and regulate maintenance of the instrument, design and implement appropriate training programs, methodology development and dissemination to the research community. To conclude, the proposed instrument acquisition will address existing deficits and positively impact NIH-funded research programs and create new collaborative venues for complementary Centers and Institutions. As such, we anticipate the user base to expand substantially with the acquisition of this new technology and implementation of training. Members of the advisory committee, the principal investigator on this proposal, and the broader user group will be involved in collectively managing future alterations to the user base and prioritization of access; the Director of the CMIA will have sole responsibility for training and compliance management. PUBLIC HEALTH RELEVANCE: The requested spinning disk confocal microscope allows the visualization of cellular processes in living cells in biologically relevant time-scale. This microscope will advance numerous research programs, including understanding of developmental neural disorders associated with intellectual deficits, the molecular basis of cancer transformation, and the molecular basis for parasitic and HIV infections. The overarching goal of these projects is to improve our understanding of the mechanisms underlying those diseased states and to design novel strategies to eradicate them.

描述(由申请人提供)：我们建议收购卡尔·蔡司细胞观察者旋转圆盘共聚焦系统，以支持乔治华盛顿大学(GW)及其合作机构由NIH资助的大量研究人员。该系统将主要用于短期和长期活细胞成像实验，以及其他当前的应用，包括高通量/高内容图像采集。该仪器的收购将克服目前用于活细胞成像的大量障碍，这是我们 现有的点扫描共聚焦显微镜。对于尖端的活细胞成像显微镜，这些是必须满足的基本技术要求，以延时记录荧光信号，以研究细胞运动、细胞器运输、自由活线虫(线虫)和小鼠基因工程胚胎。拟议的旋转圆盘显微镜技术是专门为从活样本中快速采样荧光图像量身定做的，同时提供高检测灵敏度和有限的光辐射。最大限度地减少光辐射S对大多数活细胞功能分析至关重要，以减少潜在的光毒性和活性氧化物种的产生。旋转圆盘显微镜使样品受到比目前使用的点扫描共焦显微镜低得多的光线，因此非常适合活细胞成像。许多项目将受益于获得该仪器，包括在细胞和细胞器水平上研究神经发育的分子机制，与肿瘤细胞相关的细胞周期失调的研究，研究控制钩虫发育的信号通路的分子寄生学，与艾滋病毒和转基因蠕虫寄生虫有关的宿主与病原体的相互作用，以及肝脏疾病和纤维化的肝生物学和分子基础。该仪器将集成到乔治华盛顿大学久负盛名的显微镜和图像分析中心(CMIA)的基础设施中，并向NIH资助的中心的成员提供，包括乔治华盛顿大学附属儿童国家医学中心的智力和发育障碍研究中心(IDDRC)和哥伦比亚特区艾滋病研究发展中心(DC D-CFAR)。此外，CMIA将管理和规范仪器的维护，设计和实施适当的培训计划，开发方法，并向研究界传播。总之，拟议的仪器收购将解决现有的赤字问题，并对NIH资助的研究项目产生积极影响，并为补充中心和机构创造新的合作场所。因此，我们预计随着这项新技术的获得和培训的实施，用户基础将大幅扩大。咨询委员会成员、该提案的主要调查员以及更广泛的用户群体将共同参与管理未来用户基础的变更和准入的优先次序；CMIA主任将单独负责培训和合规管理。 公共卫生相关性：所要求的旋转圆盘共聚焦显微镜允许在生物相关的时间尺度上可视化活细胞中的细胞过程。该显微镜将推进众多研究项目，包括了解与智力缺陷相关的发育神经障碍、癌症转化的分子基础，以及寄生虫和艾滋病毒感染的分子基础。这些项目的总体目标是提高我们对这些疾病状态背后的机制的了解，并设计新的战略来根除它们。