Acquisition of Zeiss 710 Confocal Laser Scanning Microscope

购买 Zeiss 710 共焦激光扫描显微镜

• 批准号: 7595495
• 负责人: Anastas Popratiloff
• 金额: $ 50万
• 依托单位: GEORGE WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-15 至 2011-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595495
• 关键词: Academic Medical Centers; Achievement; Address; Algorithms; Arts; Collaborations; Contract Services; Core Facility; Detection; Doctor of Philosophy; Energy Transfer; Environment; Equipment; Fluorescence; Fostering; Funding; Goals; Image; Image Analysis; Imagery; Institutes; Institution; Knowledge; Label; Lasers; Life; Maintenance; Methods; Microscope; Microscopic; Microscopy; Photobleaching; Process; Protocols documentation; Quantum Dots; Research; Research Personnel; Resources; Rotation; Scanning; Science; Students; Training; United States National Institutes of Health; Washington; cellular imaging; dissemination research; flexibility; fluorophore; instrument; meetings; next generation; programs; protein protein interaction; public health relevance; research study

DESCRIPTION (provided by applicant): The Center for Microscopy and Image Analysis (CMIA) at The George Washington University - Medical Center (GWUMC) provides core resources for acquisition and processing of microscopic images of relevance to biomedical sciences. The CMIA was made an institutional core facility in 1999 by a commitment of considerable institutional support. It has remained true to its original goals of research, dissemination of knowledge, and educational opportunities at several levels. CMIA offers outdated BioRad MRC 1024 ES confocal laser scanning microscopes, the maintenance of which is seriously challenged by the lack of service contracts. We now seek to acquire the next generation of confocal laser scanning microscope (CLSM) - Zeiss LSM 710. The requested CLSM will be equipped with variety laser excitation protocols and spectral emission detection, and environmental control to address current equipment deficiencies and to meet the research objectives of NIH funded investigators. In addition to the significantly higher detection efficiency and reliability compared to the existing CLSM, the proposed instrument will be capable of laser stimulation, allowing the implementation of sophisticated visualization methods such as fluorescent resonance energy transfer (FRET), photobleaching and photoconversion. Spectral detection will provide the researchers with a higher level of flexibility in selecting the emission bandwidth, elimination of cross-talk and autofluorescence. Additional laser lines (405nm) will offer labeling detection for state of the art markers such as quantum dots. Proposed instrument will be equipped with environmental control, which in combination of the greatest flexibility offered by excitation and emission protocols will advance investigators life cell imaging experiments - not possible with the current instrument. The CLSM will be the first of its class in the institution and will be capable of simultaneously detecting spectral signatures from up to eight fluorophores even if they have overlapping emission spectra, by using linear un-mixing algorithms. Proposed CLSM will advance fluorescence labeling detection, will provide us with the capability for studying protein-protein interactions, and will allow us for applying new and more reliable labeling strategies. A group of well established NIH funded investigators support this application. These investigators will be able to advance their projects beyond the limits imposed by the existing BioRad 1024ES CLSM. CMIA will use its multi-user environment for publicizing the achievements of investigators with the proposed CLSM, which will create a broad platform for methodological sharing and advancement of all NIH funded investigators. Thus, the instrument will foster interdepartmental interactions and collaborations. Training with the instrument will be available to our Institute of Biomedical Sciences PhD program students on research rotations and dissertation projects. PUBLIC HEALTH RELEVANCE: The deficits imposed by the available confocal laser scanning microscope available at The George Washington University Medical Center core facility are addressed in this application. Acquisition of a state of the art confocal laser scanning microscope is proposed, which will be integrated in into the core facility. The new instrument will allow a group of well established investigators to advance their NIH funded project.

描述（由申请人提供）：乔治华盛顿大学医学中心（GWUMC）的显微镜和图像分析中心（CMIA）为获取和处理与生物医学科学相关的显微图像提供核心资源。1999年，通过承诺提供大量的机构支助，CMIA成为一个机构核心设施。它一直忠于其研究，传播知识，并在几个层次的教育机会的最初目标。CMIA提供过时的BioRad MRC 1024 ES共聚焦激光扫描显微镜，由于缺乏服务合同，其维护面临严重挑战。我们现在寻求获得下一代共焦激光扫描显微镜（CLSM）-蔡司LSM 710。所要求的CLSM将配备各种激光激发协议和光谱发射检测，以及环境控制，以解决当前设备的缺陷，并满足NIH资助的研究人员的研究目标。除了与现有的CLSM相比显着更高的检测效率和可靠性外，拟议的仪器将能够进行激光刺激，允许实施复杂的可视化方法，如荧光共振能量转移（FRET），光漂白和光转换。光谱检测将为研究人员在选择发射带宽、消除串扰和自发荧光方面提供更高水平的灵活性。额外的激光线（405 nm）将为最先进的标记物（如量子点）提供标记检测。拟议的仪器将配备环境控制，结合激发和发射协议提供的最大灵活性，将推进研究人员的生命细胞成像实验-目前的仪器是不可能的。CLSM将是该机构同类产品中的第一个，并且能够通过使用线性非混合算法同时检测多达八个荧光团的光谱特征，即使它们具有重叠的发射光谱。提出的CLSM将推进荧光标记检测，将为我们提供研究蛋白质-蛋白质相互作用的能力，并使我们能够应用新的和更可靠的标记策略。一组由NIH资助的研究人员支持这一申请。这些研究人员将能够超越现有BioRad 1024 ES CLSM所施加的限制来推进他们的项目。CMIA将利用其多用户环境，通过拟议的CLSM宣传研究人员的成就，这将为所有NIH资助的研究人员的方法共享和进步创造一个广泛的平台。因此，该工具将促进部门间的互动与合作。该仪器的培训将提供给我们的生物医学科学研究所博士课程的学生进行研究轮换和论文项目。公共卫生关系：在本申请中解决了由乔治华盛顿大学医学中心核心设施可用的共聚焦激光扫描显微镜造成的缺陷。建议购置一台最先进的共焦激光扫描显微镜，将其集成到核心设施中。新仪器将允许一组成熟的研究人员推进他们的NIH资助项目。

项目成果

Identification and Functional Validation of Reciprocal microRNA-mRNA Pairings in African American Prostate Cancer Disparities.

• DOI: 10.1158/1078-0432.ccr-14-1566
• 发表时间: 2015-11-01
• 期刊: Clinical cancer research : an official journal of the American Association for Cancer Research
• 作者: Wang BD;Ceniccola K;Yang Q;Andrawis R;Patel V;Ji Y;Rhim J;Olender J;Popratiloff A;Latham P;Lai Y;Patierno SR;Lee NH
• 通讯作者: Lee NH

Properties of blebbistatin for cardiac optical mapping and other imaging applications.

肌球蛋白在心脏光学测绘和其他成像应用中的特性。

• DOI: 10.1007/s00424-012-1147-2
• 发表时间: 2012
• 期刊: Pflugers Archiv : European journal of physiology
• 作者: Swift,LutherM;Asfour,Huda;Posnack,NikkiG;Arutunyan,Ara;Kay,MatthewW;Sarvazyan,Narine
• 通讯作者: Sarvazyan,Narine

A New Model for Congenital Vestibular Disorders.

先天性前庭疾病的新模型。

• DOI: 10.1007/s10162-018-00705-z
• 发表时间: 2019
• 期刊: Journal of the Association for Research in Otolaryngology : JARO
• 作者: Lilian,SigmundJ;Seal,HayleyE;Popratiloff,Anastas;Hirsch,JuneC;Peusner,KennaD
• 通讯作者: Peusner,KennaD

Localization and sub-cellular shuttling of HTLV-1 tax with the miRNA machinery.

HTLV-1 与 miRNA 机制的定位和亚细胞穿梭。

• DOI: 10.1371/journal.pone.0040662
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: VanDuyne,Rachel;Guendel,Irene;Klase,Zachary;Narayanan,Aarthi;Coley,William;Jaworski,Elizabeth;Roman,Jessica;Popratiloff,Anastas;Mahieux,Renaud;Kehn-Hall,Kylene;Kashanchi,Fatah
• 通讯作者: Kashanchi,Fatah

Prostate apoptosis response protein 4 sensitizes human colon cancer cells to chemotherapeutic 5-FU through mediation of an NF kappaB and microRNA network.

• DOI: 10.1186/1476-4598-9-98
• 发表时间: 2010-04-30
• 期刊: Molecular cancer
• 影响因子: 37.3
• 作者: Wang BD;Kline CL;Pastor DM;Olson TL;Frank B;Luu T;Sharma AK;Robertson G;Weirauch MT;Patierno SR;Stuart JM;Irby RB;Lee NH
• 通讯作者: Lee NH