Preventing Persistence and Spread of Acinetobacter baumannii in Hospitals

 描述（由申请人提供）：抗生素耐药性、医院获得性细菌感染的发生率不断增加，而我们的抗生素选择越来越少，这削弱了所有医疗服务。 需要住院康复的治疗。了解细菌如何在医院中持续存在和传播对于提高抗生素耐药性医院相关细菌感染的负担至关重要。鲍曼不动杆菌是一种重要性迅速增长的医院相关病原体，并且是地方性临床污染的范例。反复发生的地方性甲型H1N1流感医院暴发。鲍曼不动杆菌菌株是有据可查的，其在各种医疗设备上的定殖以及从医院中消灭鲍曼不动杆菌的困难也是有据可查的。a.鲍曼不动杆菌强大的脱水耐受性是加剧这些问题的关键特征，然而，描述鲍曼不动杆菌脱水诱导致死和存活的潜在机制的信息很少。鲍曼不动杆菌或任何细菌。本提案的总体目标是表征氧化应激和蛋白质稳定组分对干燥耐受性的关键作用。该提案的具体目的是（1）表征干燥过程中氧化应激的作用并确定其靶点，以及（2）表征A.鲍曼氏蛋白质伴侣网络，促进干燥生存。这些目标的完成将有助于对临床环境中细菌持久性的基本知识体系的建立，并提供对A.鲍曼不动杆菌在干燥状态下存活很长时间。该提案代表了一种预防性方法，可以消除医院中病原菌的污染和传播， 但尚未充分研究的传染病控制方面。

 DESCRIPTION (provided by applicant): Increasing rates of antibiotic resistant, hospital-acquired bacterial infections, and our diminishing antibiotic options, are undercutting all medical treatments that require hospital-based recovery. Understanding how bacteria persist and spread in hospitals is paramount to elevating the burden of antibiotic resistant hospital-associated bacterial infections. Acinetobacter baumannii is a hospital-associated pathogen of rapidly growing importance, and is a paradigm for endemic clinical contamination. Reoccurring hospital outbreaks of endemic A. baumannii strains are well documented, as are its colonization of a wide array of medical equipment and the difficulty of exterminating it from hospitals. A. baumannii's robust desiccation tolerance is a key characteristic exacerbating each of these problems, however scarce information is available describing the underlying mechanisms of desiccation-induced lethality and survival for A. baumannii or any bacterium. The overall objectives of this proposal are to characterize the role of oxidative stress and proteostasis components critical for desiccation tolerance. The specific aims of this proposal are (1) to characterize the role and identify targets of oxidative stress during desiccation and (2) to characterize components of A. baumannii's protein chaperone network that promote desiccation survival. The completion of these aims will contribute to an essential body of knowledge to the persistence of bacteria in the clinical setting and provide a better understanding of the mechanisms by which A. baumannii survives for long periods of time in the desiccated state. This proposal represents a preventative approach to eradicating contamination and spread of pathogenic bacteria in hospitals and will offer a framework for future investigation of a critical, yet understudied, aspect of infectious disease control.