New Subatmospheric MALDI-MS Imaging Platform

新型低于大气压 MALDI-MS 成像平台

• 批准号: 9347389
• 负责人: EUGENE MOSKOVETS
• 金额: $ 14.43万
• 依托单位: MASSTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-05 至 2017-12-13
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9347389
• 关键词: Alkylation; Biological; Cell Culture Techniques; Cell Mobility; Chemicals; Clinical; Collection; Complex; Crystallization; Detection; Environment; Gangliosides; Gases; Generations; Goals; Image; Imaging Techniques; Implant; Ions; Lasers; Lipids; Maps; Mass Spectrum Analysis; Methods; Molecular; Morphology; Nitrogen; Optics; Peptides; Pharmaceutical Preparations; Phase; Physiologic pulse; Preparation; Proteins; Resolution; Running; Sampling; Silver; Slice; Small Business Innovation Research Grant; Source; Spatial Distribution; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spottings; System; Techniques; Thinness; Time; Tissue Sample; Tissue imaging; Tissues; Translating; United States National Institutes of Health; absorption; base; cost; design; drug distribution; experimental study; imaging platform; imaging study; improved; ionization; ionization technique; mass spectrometer; molecular mass; nanoparticle; operation; pressure; small molecule; success

The mass spectrometry imaging (MSI) is an important analytical technique broadly utilized to detect peptides, lipids, and small molecules in tissue samples with high spatial resolution. It has been started to emerge as a reliable technique in a clinical environment, particularly for characterization of drug distribution as well as morphological changes in tissues. A proposed MSI platform will combine a new UV laser and operate at subatmospheric conditions utilizing matrix-assisted laser desorption ionisation (MALDI) technique. A use of the proposed MALDI platform will drastically improve detection limits of peptides, lipids, drugs, and metabolites in the direct analysis of cell cultures and tissues.

质谱成像技术是一种重要的分析技术，应用广泛 检测具有高空间分辨率的组织样本中的肽、脂质和小分子， 分辨率它已经开始作为一种可靠的技术出现在临床环境中， 特别是用于表征药物分布以及 组织中拟议中的MSI平台将联合收割机与一种新的紫外激光器结合， 利用基质辅助激光解吸电离（MALDI）的负压条件 法使用拟议的MALDI平台将大大提高检测限 在细胞培养物和组织的直接分析中，肽、脂质、药物和代谢物的分析。