Core B: Murine Models and Gnotobiotics Core

核心 B：小鼠模型和知生素核心

• 批准号: 9197988
• 负责人: JEFFREY H MINER
• 金额: $ 12.34万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9197988
• 关键词: Alleles; Animals; Bacteria; Biomedical Research; Breeding; Control Animal; DNA; Development; Digestive System Disorders; Disease; Doxycycline; Fertilization in Vitro; Gastrointestinal tract structure; Generations; Genetic; Genotype; Germ-Free; Gnotobiotic; Health; Human; Injection of therapeutic agent; Knock-in Mouse; Knock-out; Knockout Mice; Maintenance; Methods; Movement; Mus; Mutation; Production; Research; Research Personnel; Services; Speed; Time; Transgenes; Transgenic Mice; Transgenic Organisms; Universities; Washington; assisted reproduction; blastocyst; congenic; cost effective; embryonic stem cell; gastrointestinal system; genetic pedigree; germ free condition; member; mouse model; zygote

Murine models have been extensively utilized by Washington University Digestive Diseases Research Core Center investigators and are central to DDRCC biomedical research into diverse aspects of the digestive system in development, health, and disease. The Murine Models and Gnotobiotics Core has been and will continue to be dedicated primarily to producing and maintaining genetically altered animals and to providing, deriving, and manipulating gnotobiotic mice for DDRCC members in a timely, reliable, and cost-effective manner. In addition, the Core provides ancillary services for maintaining mouse pedigrees, such as assisted reproduction, as well as rapid transfer of mutations or transgenes from one genetic background to another using "speed congenics". We will continue to offer these services in the next project period. New services to be offered include the maintenance of a Mouse Bank that will house and provide Cre and doxycycline-inducible transgenic mice of use to DDRCC members and the implementation of new, state-of-the-art methods for generating knockout and knockin mice. The Aims include: (1) Generation and propagation of transgenic, knockout, and chimeric mice for the study of digestive diseases. This includes injections of DNA into zygotes and of ES cells into blastocysts and morulae, as well as assistance with breeding, colony maintenance, rederivation of lines as specific pathogen-free, and in vitro fertilization; (2) Movement of specific alleles onto defined strain backgrounds by a "speed congenic" approach; (3) Maintenance of a "Mouse Bank" containing transgenic strains of use to DDRCC investigators; (4) Provision of unmanipulated germ-free mice and genotypically matched conventionally-raised control animals; (5) Colonization of germ-free mice with bacteria; (6) Rederivation of germ-free mice from existing conventionally-raised strains of genetically modified mice.

小鼠模型已被华盛顿大学消化系统疾病研究中心广泛使用 中心研究人员，是DDRCC对消化系统不同方面的生物医学研究的核心 发展、健康和疾病方面的系统。小鼠模型和GnotoBiotics核心已经并将继续 继续主要致力于生产和维护转基因动物，并提供 以及时、可靠和经济高效的方式为DDRCC成员派生和操作诺生菌小鼠 举止。此外，核心还为维持小鼠系谱提供辅助服务，如 繁殖，以及突变或转基因从一种遗传背景快速转移到另一种遗传背景 使用“速度基因”。在下一个项目期间，我们将继续提供这些服务。提供新服务 将提供包括维护一个鼠库，该库将容纳和提供CRE和强力霉素诱导剂 用于DDRCC成员的转基因小鼠和实施新的、最先进的 产生基因敲除和敲除小鼠的方法。 目的包括：(1)用于研究的转基因、基因敲除和嵌合小鼠的产生和繁殖 消化系统疾病。这包括将DNA注射到受精卵中，将ES细胞注射到囊胚中，并 桑树，以及协助育种、群体维持、特定品系的重新衍生 无病原体和体外受精；(2)通过一种特定的等位基因转移到特定的菌株背景 “速度同源”方法；。(3)维持含有转基因品系的“鼠库”。 DDRCC调查员；(4)提供未经处理的无菌小鼠和基因配型匹配的小鼠 常规饲养的对照动物；(5)无菌小鼠带菌定居；(6)重新衍生 从现有的常规饲养的转基因小鼠品系中分离出无菌小鼠。