Critical resources provided by UNMC RAP biorepository stimulate cancer research

UNMC RAP 生物样本库提供的关键资源促进癌症研究

• 批准号: 9353742
• 负责人: Paul M Grandgenett
• 金额: $ 10.68万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-16 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9353742
• 关键词: Address; Archives; Ascites; Autopsy; Award; Blood; Cancer Center; Cancer Research Project; Cell Culture Techniques; Cessation of life; Collaborations; Collection; DNA; Disease; Distant Metastasis; Drug or chemical Tissue Distribution; Early Detection Research Network; Freezing; Funding; Grant; Harvest; Hour; Human; Individual; Institution; International; Knowledge; Laboratories; Leadership; Lesion; Liquid substance; Malignant neoplasm of pancreas; Medical center; Molecular; Nebraska; Neoplasm Circulating Cells; Organ; Organoids; Pancreas; Patients; Play; Premalignant; Primary Neoplasm; Procedures; Process; Proteins; RNA; Recording of previous events; Research; Research Assistant; Research Personnel; Research Project Grants; Research Training; Residual state; Resources; Role; Sampling; Ships; Specialist; Specimen; TNFRSF5 gene; Time; Tissues; Universities; Work; Xenograft procedure; anticancer research; base; biobank; blood-based biomarker; experience; innovation; professor; programs; repository; sample collection; success; tumor microenvironment

Project Summary The highly innovative Rapid Autopsy Pancreas (RAP) program addresses a major limitation in pancreatic cancer research: the lack of quality human specimens for pancreatic cancer representing all stages of disease. Our program of rapid collection of large quantities of all organs and fluids that initiates within an hour of death and is completed within five hours is a unique program in the world. The repository generated from these autopsies allows for the capture of the complete disease history present at the time death and ultimately allows for collection of samples representing the full spectrum of disease from premalignant lesions, primary tumor and metastatic disease. The RAP program is a core component of the University of Nebraska Medical Center (UNMC) Pancreatic Cancer Research program that is tasked with the collection, archiving and distribution of tissues and fluids from patients that have recently passed with pancreatic cancer. The Unit Director of the Pancreatic Cancer Research program at the Fred and Pamela Buffett Cancer Center of UNMC is Dr. Michael A. Hollingsworth. Dr. Paul Grandgenett, a Research Assistant Professor under the aegis of Dr. Hollingsworth's, is the director of the RAP program and the applicant for this Research Specialist Award. The RAP program operates within the Pancreatic Cancer Research Program at UNMC, and is important to and currently funded in part by: a pancreatic cancer SPORE p50 grant; ongoing and pending U01 grants from the Early Detection Research Network; a U54 grant as part of the Tumor Microenvironment Network; and several R01 grants to individual investigators. A key role of Dr. Grandgenett is to work with collaborators to determine the samples that are needed for a research project, to modify our SOP as needed to obtain those samples, and then to provide those samples to investigators. It is essential for the leadership of this resource to be a highly trained Research Specialist with extensive experience through participation in the autopsies, a comprehensive knowledge of the patient samples available, as well as the ability to interact on a scientific level with each investigator. Specimens collected include primary tumor, residual unaffected pancreas, distant metastases, all unaffected tissues, as well as fluids such as blood and ascites. These tissues and fluids are rapidly harvested under an SOP that aims to maintain cellular and molecular integrity of the tissue. Selected fresh samples are processed immediately or used for live cell culture/xenograft procedures. These procedures are determined in advance and are based on the needs of funded projects both at UNMC and at other institutions. Currently, we ship fresh samples to other laboratories conducting research on blood based biomarkers (proteins, RNA, DNA), circulating tumor cells, cell cultures including organoids, and other processes. The remaining samples are frozen, maintained in long-term storage facilities and made available through request and collaboration for local, national and international pancreatic cancer studies. Dr. Grandgenett has a major role in all aspects of the collection, processing, annotation and distribution of these biospecimens.

项目摘要 高度创新的快速尸检胰腺（RAP）计划解决了胰腺的主要限制 癌症研究：缺乏代表疾病所有阶段的胰腺癌的优质人类标本。 我们的快速收集大量的所有器官和流体计划，这些器官和流体在死亡后一个小时内启动 在五个小时内完成是世界上独特的计划。从这些生成的存储库 尸检允许捕获死亡时出现的完整疾病病史，并最终允许 用于收集代表预先病变的全部疾病的样品，原发性肿瘤 和转移性疾病。说唱计划是内布拉斯加州大学医学中心的核心组成部分 （UNMC）胰腺癌研究计划，其任务是收集，归档和分发 最近患有胰腺癌的患者的组织和液体。单位主任 弗雷德（Fred）和帕梅拉·巴菲特（Pamela Buffett）癌症中心的胰腺癌研究计划是迈克尔博士 A. Hollingsworth。 Paul Grandgenett博士是Hollingsworth博士的宙斯盾的研究助理教授， 是RAP计划的主任，也是该研究专家奖的申请人。说唱程序 在UNMC的胰腺癌研究计划中运营，对目前的资助很重要 部分作者：胰腺癌孢子P50赠款；从早期检测中进行的和待定的U01赠款 研究网络； U54赠款作为肿瘤微环境网络的一部分；以及几个R01赠款 个人调查员。 Grandgenett博士的关键作用是与合作者合作以确定样本 研究项目需要的是，以获取这些样本的需要修改我们的SOP，然后 将这些样品提供给研究人员。对于该资源的领导才能成为经验丰富的训练 通过参与尸检，具有丰富经验的研究专家，这是一个全面的 了解可用的患者样本，以及与每个患者相互作用的能力 研究者。收集的标本包括原发性肿瘤，残留的未影响胰腺，远处转移，所有 未受影响的组织以及血液和腹水等液体。这些组织和流体迅速收获 在SOP下，旨在维持组织的细胞和分子完整性。选定的新鲜样品是 立即处理或用于活细胞培养/异种移植程序。这些程序是在 进步，并基于UNMC和其他机构的资助项目的需求。目前，我们 将新鲜样品运送到其他实验室，从 DNA），循环肿瘤细胞，包括器官和其他过程在内的细胞培养物。其余样品 被冷冻，维护在长期存储设施中，并通过要求和协作提供 地方，国家和国际胰腺癌研究。 Grandgenett博士在各个方面都有重要角色 这些生物测量的收集，处理，注释和分布。