Critical resources provided by UNMC RAP biorepository stimulate cancer research

UNMC RAP 生物样本库提供的关键资源促进癌症研究

• 批准号: 9353742
• 负责人: Paul M Grandgenett
• 金额: $ 10.68万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-16 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9353742
• 关键词: Address; Archives; Ascites; Autopsy; Award; Blood; Cancer Center; Cancer Research Project; Cell Culture Techniques; Cessation of life; Collaborations; Collection; DNA; Disease; Distant Metastasis; Drug or chemical Tissue Distribution; Early Detection Research Network; Freezing; Funding; Grant; Harvest; Hour; Human; Individual; Institution; International; Knowledge; Laboratories; Leadership; Lesion; Liquid substance; Malignant neoplasm of pancreas; Medical center; Molecular; Nebraska; Neoplasm Circulating Cells; Organ; Organoids; Pancreas; Patients; Play; Premalignant; Primary Neoplasm; Procedures; Process; Proteins; RNA; Recording of previous events; Research; Research Assistant; Research Personnel; Research Project Grants; Research Training; Residual state; Resources; Role; Sampling; Ships; Specialist; Specimen; TNFRSF5 gene; Time; Tissues; Universities; Work; Xenograft procedure; anticancer research; base; biobank; blood-based biomarker; experience; innovation; professor; programs; repository; sample collection; success; tumor microenvironment

Project Summary The highly innovative Rapid Autopsy Pancreas (RAP) program addresses a major limitation in pancreatic cancer research: the lack of quality human specimens for pancreatic cancer representing all stages of disease. Our program of rapid collection of large quantities of all organs and fluids that initiates within an hour of death and is completed within five hours is a unique program in the world. The repository generated from these autopsies allows for the capture of the complete disease history present at the time death and ultimately allows for collection of samples representing the full spectrum of disease from premalignant lesions, primary tumor and metastatic disease. The RAP program is a core component of the University of Nebraska Medical Center (UNMC) Pancreatic Cancer Research program that is tasked with the collection, archiving and distribution of tissues and fluids from patients that have recently passed with pancreatic cancer. The Unit Director of the Pancreatic Cancer Research program at the Fred and Pamela Buffett Cancer Center of UNMC is Dr. Michael A. Hollingsworth. Dr. Paul Grandgenett, a Research Assistant Professor under the aegis of Dr. Hollingsworth's, is the director of the RAP program and the applicant for this Research Specialist Award. The RAP program operates within the Pancreatic Cancer Research Program at UNMC, and is important to and currently funded in part by: a pancreatic cancer SPORE p50 grant; ongoing and pending U01 grants from the Early Detection Research Network; a U54 grant as part of the Tumor Microenvironment Network; and several R01 grants to individual investigators. A key role of Dr. Grandgenett is to work with collaborators to determine the samples that are needed for a research project, to modify our SOP as needed to obtain those samples, and then to provide those samples to investigators. It is essential for the leadership of this resource to be a highly trained Research Specialist with extensive experience through participation in the autopsies, a comprehensive knowledge of the patient samples available, as well as the ability to interact on a scientific level with each investigator. Specimens collected include primary tumor, residual unaffected pancreas, distant metastases, all unaffected tissues, as well as fluids such as blood and ascites. These tissues and fluids are rapidly harvested under an SOP that aims to maintain cellular and molecular integrity of the tissue. Selected fresh samples are processed immediately or used for live cell culture/xenograft procedures. These procedures are determined in advance and are based on the needs of funded projects both at UNMC and at other institutions. Currently, we ship fresh samples to other laboratories conducting research on blood based biomarkers (proteins, RNA, DNA), circulating tumor cells, cell cultures including organoids, and other processes. The remaining samples are frozen, maintained in long-term storage facilities and made available through request and collaboration for local, national and international pancreatic cancer studies. Dr. Grandgenett has a major role in all aspects of the collection, processing, annotation and distribution of these biospecimens.

项目摘要 高度创新的快速尸检胰腺（RAP）计划解决了胰腺癌的主要局限性， 癌症研究：缺乏代表疾病所有阶段的胰腺癌高质量人体标本。 我们的计划是在死亡后一小时内开始大量收集所有器官和体液 在五个小时内完成，是世界上独一无二的项目。从这些生成的存储库 尸体解剖允许捕获死亡时存在的完整疾病史，并最终允许 用于收集代表癌前病变、原发性肿瘤 和转移性疾病。RAP计划是内布拉斯加大学医学中心的核心组成部分 （UNMC）胰腺癌研究计划，其任务是收集，存档和分发 最近死于胰腺癌的病人的组织和体液。该股股长 UNMC弗雷德和帕梅拉·巴菲特癌症中心的胰腺癌研究项目是迈克尔博士 A.霍林斯沃思Paul Grandgenett博士是Hollingsworth博士的研究助理教授， 是RAP计划的主任，也是该研究专家奖的申请人。RAP计划 在UNMC的胰腺癌研究计划内运作，对目前资助的胰腺癌研究计划很重要。 部分原因是：胰腺癌SPORE p50补助金;正在进行和即将进行的早期检测U 01赠款 研究网络;作为肿瘤微环境网络一部分的U 54赠款;以及几个R 01赠款， 个别调查员。Grandgenett博士的一个关键作用是与合作者一起确定样本 研究项目所需的样本，根据需要修改我们的SOP以获得这些样本，然后 把这些样本交给调查人员这一资源的领导必须是训练有素的 研究专家通过参与尸检，全面的经验丰富， 了解可用的患者样本，以及在科学层面上与每个患者进行互动的能力。 调查员收集的标本包括原发性肿瘤、残留的未受影响的胰腺、远处转移瘤、所有 未受影响的组织，以及液体，如血液和腹水。这些组织和体液被迅速采集 根据旨在保持组织的细胞和分子完整性的SOP。所选新鲜样品为 立即处理或用于活细胞培养/异种移植程序。这些程序在 这是根据联合国监测委员会和其他机构的供资项目的需要预先确定的。目前我们 将新鲜样本运送到其他实验室进行基于血液的生物标志物（蛋白质，RNA， DNA）、循环肿瘤细胞、包括类器官的细胞培养物和其他过程。剩余样品 冷冻，保存在长期储存设施中，并通过请求和合作提供， 地方、国家和国际胰腺癌研究。Grandgenett博士在各个方面都发挥着重要作用， 这些生物标本的收集、加工、注释和分发。