Critical Resources Provided by UNMC RAP Biorepository Stimulate Cancer Research

UNMC RAP 生物样本库提供的关键资源促进癌症研究

• 批准号: 10323146
• 负责人: Paul M Grandgenett
• 金额: $ 14.42万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-15 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10323146
• 关键词: Address; Archives; Ascites; Autopsy; Award; Blood; Cancer Center; Cancer Patient; Cancer Research Project; Cessation of life; Collaborations; Collection; DNA; Disease; Distant Metastasis; Drug or chemical Tissue Distribution; Focus Groups; Freezing; Funding; Grant; Hour; Human; Individual; Institution; International; Knowledge; Laboratories; Leadership; Liquid substance; Malignant Neoplasms; Malignant neoplasm of pancreas; Malignant neoplasm of prostate; Medical center; Modeling; Molecular; Nebraska; Neoplasm Circulating Cells; Normal tissue morphology; Oncology; Organ; Organoids; Patients; Play; Postdoctoral Fellow; Primary Neoplasm; Process; Prostate; Proteins; RNA; Research; Research Personnel; Research Project Grants; Research Training; Residual state; Resources; Role; Sampling; Specialist; Specimen; TNFRSF5 gene; Tissues; Training; Universities; Work; Xenograft procedure; anticancer research; base; biobank; blood-based biomarker; design; experience; innovation; mid-career faculty; programs; success; volunteer

Project Summary The highly innovative Rapid Autopsy Program (RAP) addresses a major limitation in pancreatic and prostate cancer research: the lack of quality human specimens of cancer representing all stages of disease. This program, led by Dr. Paul M. Grandgenett, which includes rapid collection of large quantities of all organs and fluids within 5 hours of death, is a unique program in the world and is designed to maintain cellular and molecular integrity of the tissue. The RAP is a core component of the University of Nebraska Medical Center (UNMC) Pancreatic Cancer Research program and the GU Oncology Focus Group, that is tasked with the collection, archiving and distribution of tissues and fluids from patients that have recently passed with pancreas or prostate cancer. The Unit Director of the Pancreatic Cancer Research program at the Fred and Pamela Buffett Cancer Center is Dr. Michael A. Hollingsworth, while the prostate group is led by Dr. Surinder Batra and Dr. Shawna Boyle. Dr. Grandgenett, a Research Associate Professor under Dr. Hollingsworth's aegis, is the director of the RAP and the applicant for this Research Specialist Award. The rapid autopsy program operates within the Pancreatic Cancer Research Program at UNMC and is important to and currently funded in part by: a pancreatic cancer SPORE p50 grant; ongoing and pending P01 and U01 grants and several R01 grants to individual investigators. Dr. Grandgenett personally directs the collection of primary tumor, residual unaffected tissues, distant metastases, all unaffected tissues, as well as fluids such as blood and ascites for all PDAC and Prostate cancer patients. He has also established an extensive normal tissue acquisition program that is highly utilized. Dr. Grandgenett determines in advance how specific samples will be prioritized based on the needs of funded projects both at UNMC and at other institutions. Currently, Dr. Grandgenett provides fresh samples to multiple laboratories, at UNMC and outside-UNMC, conducting research on blood-based biomarkers (proteins, RNA, DNA), circulating tumor cells, patient-derived modeling including organoids and xenografts, and other processes. The remaining samples are snap frozen, maintained in long-term storage and made available through request and collaboration for local, national and international cancer studies. Key roles of Dr. Grandgenett is to coordinate the training and participation of the thirty RAP volunteers and to work with collaborators to determine the samples necessary for their research project, to modify our SOP as needed to obtain those samples, confirm specimen quality and then to distribute those samples to investigators. It is essential for the leadership of this resource to be a highly trained Research Specialist with extensive experience through participation in the autopsies, a comprehensive knowledge of patient sample availability, as well as the ability to interact on a scientific level with each investigator. Dr. Grandgenett has a major role in all aspects of the collection, processing, annotation and distribution of these biospecimens and plays a vital role in expanding collaborative projects with investigators at UNMC and on a national and international level.

项目摘要 高度创新的快速尸检计划（RAP）解决了胰腺和前列腺的主要限制 癌症研究：缺乏代表疾病所有阶段的癌症的优质人类标本。这 Paul M. Grandgenett博士领导的计划，其中包括大量所有器官的收集 死亡后5小时内的流体是世界上一个独特的计划，旨在维持细胞和 组织的分子完整性。说唱是内布拉斯加州大学医学中心的核心组成部分 （UNMC）胰腺癌研究计划和GU肿瘤学焦点小组，其任务是 最近与胰腺经过的患者的组织和流体的收集，归档和分布 或前列腺癌。弗雷德和帕梅拉的胰腺癌研究计划的单位主任 巴菲特癌症中心是迈克尔·霍林斯沃思（Michael A. Hollingsworth博士 Shawna Boyle博士。霍林斯沃思（Hollingsworth）博士的研究副教授Grandgenett博士是 RAP董事和该研究专家奖的申请人。快速尸检计划运行 在UNMC的胰腺癌研究计划中，对于以下部分非常重要，目前由以下方式资助： 胰腺癌孢子P50补助金；正在进行的和未决的P01和U01赠款以及几项R01赠款 个人调查员。 Grandgenett博士亲自指示原发性肿瘤的收集，残留不受影响 组织，远处转移，所有未受影响的组织，以及所有PDAC的血液和腹水等液体 前列腺癌患者。他还建立了一个广泛的正常组织采集计划 利用。 Grandgenett博士提前确定如何根据需求确定特定样本的优先级 在UNMC和其他机构的资助项目。目前，Grandgenett博士为 在UNMC和UNMC的多个实验室，对基于血液的生物标志物进行研究（蛋白质，， RNA，DNA），循环肿瘤细胞，包括器官和异种移植物的患者衍生建模以及其他 过程。其余样品被冻结，在长期存储中维护并提供 通过本地，国家和国际癌症研究的要求和协作。博士的关键角色 格兰杰特（Grandgenett）将协调30名说唱志愿者的培训和参与，并与之合作 合作者确定其研究项目所需的样本，以根据需要修改我们的SOP 获取这些样品，确认标本质量，然后将这些样品分配给研究人员。这是 对于该资源的领导才能成为一名训练有素的研究专家至关重要的 通过参与尸检的经验，对患者样本可用性的全面了解， 以及与每个研究者在科学水平上进行互动的能力。 Grandgenett博士在 收集，处理，注释和分布的所有方面，这些生物测量并发挥至关重要 在与UNMC以及国家和国际层面的调查人员扩大合作项目中的作用。