Silk-based biospecimen stabilization for exposure assessment diagnostics

用于暴露评估的丝基生物样本稳定化 项目概要/摘要 迫切需要一种稳定平台，该稳定平台可以保持生物样本的完整性，完全不受环境影响。 冷藏要求。这种技术将使研究人员、临床医生、政府人员和 消费者可以在现场条件下进行生物监测（即无需前往门诊实验室）。我们 长期目标是开发一种平台技术，最大限度地提高各种生物标本的稳定性 在一种新型的丝蛋白基质中，并与下游分析无缝集成。的目的 目前的建议是建立一种能够捕获已知体积的样本、整合 与我们的第一阶段计划中研究的丝基质稳定剂，并允许立即储存， 出货时通过使用干燥剂干燥。我们的中心假设是，可以设计一种新的收集装置， 为了克服当前样品的实际设计导向和基本技术限制 收藏选择我们的假设是根据我们最近发表和未发表的数据制定的 使用蚕丝来稳定一系列血清/血浆蛋白，以及对加工技术的新见解， 蚕丝需要在高温下长时间储存和运输。因此，在第一个目标中，我们将 开发一种新的收集装置，以适应新的丝绸特征。作为第二个目标， 将招募静脉血、毛细血管血和唾液样本，将其等分并储存在丝中 系统、干纸或作为液体等分试样进行各种处理延迟，以模拟远程运输 用例在第三个目标中，将对从现有印度队列中抽取的200名受试者进行评估， 危险暴露（Vaxess）和肝脏、甲状腺和心血管疾病生物标志物的血浆水平（Agada 医学）。预期的结果将是丝绸稳定和新的经济增长背后更广泛的基本面基础。 将这些发现转化为生物标本回收和储存的方法，因为它们与生物监测有关。 拟议研究的基本原理是，一旦收集设备得到验证，它们就可以用于 各种外部伙伴关系。计划方法的成功可以对许多领域产生积极影响， 诊断学和流行病学领域的需求。这些需求包括solid的替代方法 生物样本稳定化，以及对危害接触重要的分析技术的翻译/验证。 此外，通过提供与整体的手指针刺体积（即，侵入性较小的量）接口的方法， 血液，基于丝绸的稳定系统可以显着减少与血液相关的经济负担， 门诊部，并反过来增加可及性。

Silk-Based Biospecimen Stabilization for Exposure Assessment Project Summary/Abstract There is a critical need for a stabilization platform that can preserve biospecimen integrity, completely free of cold storage requirements. Such a technology would allow researchers, clinicians, government personnel and consumers to conduct biomonitoring in field conditions (i.e. without requiring visits to the outpatient lab). Our long-term goal is to develop a platform technology that maximizes the stability of a wide range of biospecimens in a novel silk protein matrix and that seamlessly integrates with downstream analytics. The objective of the current proposal is to build a novel collection device capable of capturing known volumes of specimen, integrating with the silk matrix stabilizer investigated in our Phase I program, and allowing for immediate storage and shipment via the use of desiccant drying. Our central hypothesis is that a new collection device can be designed in order to overcome both practical design-oriented and fundamental technological limitations of current sample collection options. Our hypothesis has been formulated based on our recent published and unpublished data using silk to stabilize a range of serum/plasma proteins and new insights into processing techniques that allow silk to be stored and shipped for extended durations at elevated temperatures. Therefore, in the first aim we will develop a novel collection device to accommodate the new silk feature. As a second aim, a 50-person cohort will be recruited for venous blood, capillary blood, and saliva samples, to be aliquoted and stored in the silk system, dried paper, or as liquid aliquots subject to various processing delays to simulate remote transportation use cases. In the third aim, two hundred subjects taken from an existing Indian cohort will be evaluated for hazard exposure (Vaxess) and plasma levels of liver, thyroid, and cardiovascular disease biomarkers (Agada medical). The expected outcomes would be a broader fundamental basis behind silk stabilization and new methods to translate these findings towards biospecimen recovery and storage as they pertain to biomonitoring. The rationale for the proposed research is that, once collection devices are validated, they can be used in a variety of external partnership settings. Success with the planned approach can positively impact many areas of need in the field of diagnostics and epidemiology. These needs include alternative approaches at solid biospecimen stabilization, and translation/validation of analytical techniques important for hazard exposures. Furthermore, by offering methods to interface with finger-prick volumes (i.e. less invasive quantities) of whole blood, a silk-based stabilization system could significantly reduce financial burdens associated with the outpatient clinic, and in turn increase accessibility.