Position effects on gene expression level and noise
位置对基因表达水平和噪声的影响
基本信息
- 批准号:9342995
- 负责人:
- 金额:$ 30.1万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-09-01 至 2020-05-31
- 项目状态:已结题
- 来源:
- 关键词:AniridiaBiological ModelsBiotechnologyBrainCellsCessation of lifeChromatinChromosomal RearrangementCodeDNA Replication TimingDataDiseaseEmbryoEnvironmentEpigenetic ProcessEssential GenesEuchromatinEukaryotaEvolutionFluorescence-Activated Cell SortingGalactoseGalectin 1Gene ClusterGene DuplicationGene ExpressionGene Expression RegulationGene ProteinsGene StructureGenesGeneticGenomeGenomic SegmentGenomicsHereditary DiseaseHeterochromatinHoloprosencephalyHumanIndividualInfertilityIrisKnowledgeLifeLightLinkLocationMeasuresMetabolic PathwayModelingMolecularMolecular GeneticsNoiseNucleosomesOrganPathway interactionsPatternPhenotypePositioning AttributeProteinsResearchRoleSaccharomyces cerevisiaeSaccharomycetalesSeriesTestingTimeTissuesTransgenesTransgenic OrganismsVariantYeastschromosomal locationdeafnessdevelopmental diseasedriving forceduplicate genesexperimental studyfitnessgenetic technologygenome-widehistone modificationhuman diseasemutantprogramspromoterprotein complexprotein expressionpublic health relevancescale upsexsuccesssynthetic biologytraittransgene expressionwhole genome
项目摘要
Project Summary
The long-term objective of my research program is to understand the molecular genetic mechanisms and
driving forces of phenotypic variation and evolution, where the phenotype may be at molecular, cellular, tissue,
organ, organismal or other levels. Gene expression is a phenotypic trait at the molecular level that is critical to
all forms of life. This proposal centers on one aspect of gene expression known as position effect, which refers
to the influence of the chromosomal location of a gene on its activity. Position effect has been implicated in a
number of genetic diseases such as sex reversal, aniridia (absence of iris), and holoprosencephaly (a brain
developmental disorder that typically leads to embryonic death). Any genetic disease caused by a
chromosomal rearrangement that does not disrupt gene structure may be regarded as a result of position
effect. Position effect has been observed many times in transgenic organisms, revealing the significance of the
location at which the transgene is placed in a genome on its expression. Position effect has also been invoked
in the explanations of multiple nonrandom features of genome organization. Despite its importance, our
knowledge about the pattern and mechanism of position effect is limited and many hypotheses regarding
position effect remain untested. Past studies of position effect focused on the role of heterochromatin, largely
ignoring euchromatin where the vast majority of genes are located (and translocated in mutants and in
evolution). A genomic study of position effects on mean protein expression and expression noise in the
budding yeast Saccharomyces cerevisiae is proposed. The generated data will be used to probe the general
patterns of position effects, to study the underlying genetic and epigenetic mechanisms of position effects, and
to test the role of position effects in the formation of nonrandom features of genome organization. Furthermore,
a series of manipulative experiments will be conducted in yeast to test a previously unrecognized role of
position effect on expression noise in the origin of chromosomal clustering of functionally related genes.
Overall, this project is expected to (i) provide unprecedented genome-wide information of position effect on
gene expression and expression noise in yeast, (ii) uncover the molecular and mechanistic basis of position
effects, and (iii) deepen our understanding of the role of position effect in the evolution of eukaryotic genome
organization.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JIANZHI ZHANG其他文献
JIANZHI ZHANG的其他文献
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{{ truncateString('JIANZHI ZHANG', 18)}}的其他基金
Genomic and systemic approaches to evolutionary mechanisms
进化机制的基因组和系统方法
- 批准号:
10551882 - 财政年份:2021
- 资助金额:
$ 30.1万 - 项目类别:
Equipment Supplement: Genomic and Systemic Approaches of Evolutionary Mechanisms
设备补充:进化机制的基因组和系统方法
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Pleiotropy: patterns, mechanisms, and evolutionary consequences
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