Animal Models & Phenotyping Core

动物模型

• 批准号: 9266738
• 负责人: Randall Lee Mynatt
• 金额: $ 26.26万
• 依托单位: LSU PENNINGTON BIOMEDICAL RESEARCH CTR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9266738
• 关键词: Address; Affect; Alleles; Animal Behavior; Animal Model; Animals; Behavioral; Comparative Biology; Constitution; Consultations; DNA; Deposition; Development; Diabetes Mellitus; Diet; Eating; Elements; Energy Metabolism; Environment; Equipment; Exposure to; Faculty; Fatty acid glycerol esters; Functional disorder; Funding; Gene Expression; Gene Targeting; Generations; Genes; Genetic; Genomics; Goals; Health; High Fat Diet; Human; Insulin Resistance; Investigation; Knockout Mice; Louisiana; Measures; Metabolic; Metabolism; Methods; Mission; Monitor; Mus; Nutritional Study; Obesity; Pancreas; Phase; Phenotype; Physiological; Procedures; Production; Productivity; Research; Research Personnel; Resources; Rodent Model; Role; Scientist; Services; Techniques; Technology; Tissues; Training; Transgenic Organisms; Ursidae Family; Work; base; cellular imaging; experimental study; flexibility; genetic manipulation; human disease; in vivo; innovation; insight; knockout gene; member; metabolic phenotype; mouse model; novel; nutrition; obesogenic; programs; promoter; sedentary lifestyle; translational approach

D. Abstract (Animal Models & Phenotyping Core) During the past 10 years (Y1-5 and Y6-10 of funding), the mandate of the Animal Models and Phenotyping Core (AMPC) was to make high quality transgenic and gene knockout mouse production and metabolic and behavioral phenotyping readily accessible, both technically and financially, to NORC members. The Animal Models and Phenotyping Core (AMPC) is comprised of two interactive components: the Animal Models Subcore and the Energy Metabolism and Behavioral Subcore. THE AMPC provides services that combined controlled manipulation of gene expression in mice with state-of-the-art in vivo metabolic phenotyping and detailed behavioral analysis. Over the past two funding cycles (Y1-5, Y6-10), the APMC has been a key component of the overall mission of the Pennington NORC to stimulate new and innovative research related to nutrition and obesity. The aims of the core are as follows: Aim 1: To utilize transgenic and gene targeting techniques to generate mouse models that mimic human disease states, such as obesity and insulin resistance, when exposed to an obeseogenic environment (sedentary lifestyle and high caloric diets). Aim 2: To conduct detailed in vivo metabolic phenotyping to assess measures of physiologic function such as, energy expenditure, fat accumulation, food intake, and metabolic flexibility. Aim 3: Conduct in vivo experiments for detailed analysis of animal behavior so as to support NORC members and the Pilot and Feasibility Program. Aim 4: Pursue new methods and experimental paradigms based on observations from NORC members and Pilot and Feasibility experiments. The above Aims will be performed using a combination of genetic and other in vivo approaches. Experiments in which the genetic constitution can be changed to study the role of specific genes on obesity and diabetes cannot be performed in humans. These studies will identify mechanisms that act on the progression of metabolic dysfunction (i.e., insulin resistance, ectopic fat deposition, metabolic inflexibility, pancreatic dysfunction, etc) by using rodent models that develop obesity and insulin resistance through direct genetic manipulation or exposure to high fat diets. Thus, through rigorous in vivo metabolic phenotyping in conjunction with detailed mechanistic analysis of relevant tissues by NORC investigators, the AMPC helps all NORC projects address the critical unanswered question of how an obesogenic environment affects normal metabolism at the whole body level.

D. 摘要（动物模型和表型分析核心） 在过去 10 年中（第 1-5 年和第 6-10 年的资助），动物模型和表型分析的任务 核心（AMPC）是制造高品质转基因和基因敲除小鼠的生产和代谢及 NORC 成员在技术和经济上都可以轻松获得行为表型分析。动物 模型和表型分析核心 (AMPC) 由两个交互组件组成：动物模型 子核心以及能量代谢和行为子核心。 AMPC 提供的服务结合了 通过最先进的体内代谢表型对小鼠基因表达进行控制操作 详细的行为分析。在过去的两个资助周期（Y1-5、Y6-10）中，APMC 一直是关键 Pennington NORC 总体使命的一部分是促进与以下领域相关的新的和创新的研究 营养和肥胖。核心目标如下： 目标 1：利用转基因和基因靶向 生成模拟人类疾病状态（例如肥胖和胰岛素）的小鼠模型的技术 当暴露于致肥胖环境（久坐的生活方式和高热量饮食）时，会产生抵抗力。目标 2： 进行详细的体内代谢表型分析，以评估生理功能的测量，例如能量 支出、脂肪积累、食物摄入和代谢灵活性。目标 3：进行体内实验 详细分析动物行为，以支持 NORC 成员以及试点和可行性计划。 目标 4：根据 NORC 成员和研究人员的观察，寻求新方法和实验范式 试点和可行性实验。上述目标将通过遗传和其他因素的结合来实现 体内方法。可以改变遗传构成以研究特定作用的实验 肥胖和糖尿病的基因无法在人类身上进行。这些研究将确定机制 作用于代谢功能障碍的进展（即胰岛素抵抗、异位脂肪沉积、代谢 通过使用产生肥胖和胰岛素抵抗的啮齿动物模型来治疗不灵活、胰腺功能障碍等） 通过直接基因操作或接触高脂肪饮食。因此，通过严格的体内代谢 NORC 研究人员对表型进行分析并结合相关组织的详细机制分析， AMPC 帮助所有 NORC 项目解决尚未解答的关键问题：肥胖环境如何影响肥胖？ 影响全身的正常新陈代谢。