Molecular Genetics of Thermogenesis

生热作用的分子遗传学

• 批准号: 8067076
• 负责人: Randall Lee Mynatt
• 金额: $ 39万
• 依托单位: LSU PENNINGTON BIOMEDICAL RESEARCH CTR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8067076
• 关键词: Adipose tissue; Adult; Alleles; Animals; Binding; Binding Proteins; Biopsy; Body Temperature; Brown Fat; Burn injury; Calories; Cardiovascular Diseases; Cell Culture System; Cell Culture Techniques; Cells; Characteristics; Cytoplasm; Development; Diet; Down-Regulation; EF Hand Motifs; EF-Hand Domain; Ear; Eating; Embryo; Energy Intake; Energy Metabolism; Energy Metabolism Pathway; Fatty acid glycerol esters; Fiber; Fibroblasts; Gene Expression; Gene Expression Profile; Generations; Genes; Genetic Models; Genetically Engineered Mouse; Genus Hippocampus; Glycerol; Glycerol-3-Phosphate Dehydrogenase; Grant; Heart; Heating; Homeostasis; Human; Image; In Vitro; Indirect Calorimetry; Inner mitochondrial membrane; Lead; Location; Maintenance; Measures; Mediating; Mesenchymal Stem Cells; Metabolic; Metabolism; Mitochondria; Molecular Genetics; Mus; Muscle; Muscle Cells; Muscle Contraction; Muscle Fatigue; Muscle function; Mutant Strains Mice; Mutation; Myocardial; Myocardium; Obesity; Oxidoreductase; Pathway interactions; Phenotype; Physical Endurance; Physical activity; Physiologic Thermoregulation; Physiological; Physiological Processes; Physiology; Play; Predisposition; Production; Property; Proteins; Regulation; Resistance; Respiration; Role; SV40 T Antigens; Sarcoplasmic Reticulum; Skeletal Muscle; Stress; Structure; System; Testing; Thermogenesis; Tissues; Up-Regulation; Work; base; design; diabetic; diabetic patient; energy balance; extracellular; in vivo; inorganic phosphate; insulin sensitivity; mitochondrial uncoupling protein; mutant; novel; null mutation; nutrition; oxidation; protein expression; public health relevance; research study; response; subcutaneous

DESCRIPTION (provided by applicant): A positive energy balance that occurs when energy intake exceeds energy expenditure is an essential physiological condition for the development obesity. While much is known of the basic mechanisms controlling food intake, almost nothing is known of the identity of thermogenic mechanisms, apart from physical activity, that could be activated to burn off excess calories. We have forced mice, in which the mitochondrial uncoupling protein1 (UCP1) has been ablated, to activate alternative mechanisms of thermogenesis by gradually exposing them to the cold. Using several genetic models of UCP1 deficiency we have found that a novel gene, Slc25a25, has been consistently induced in skeletal muscle and inguinal fat under conditions of cold stress and diet-induced obesity (DIO). Since Slc25a25 encodes a putative ATP-Mg2+/Pi transporter, which is located on the inner mitochondrial membrane where it is thought to be involved in the regulation of ATP levels in the mitochondria, we have pursued experiments to test the hypothesis that Slc25a25 is involved in energy homeostasis. Mice with a global inactivation of Slc25a25 are resistant to DIO and have reduced physical endurance when tested on a treadmill. Based upon the fact that SLC25A25 has Ca2+-binding EF-hand domains and its ATP-Mg2+/Pi activity is regulated by Ca2+, we have designed experiments to test the hypothesis that SLC25A25 plays a crucial role in regulation of optimal energy levels for muscle excitation- contraction. In addition, a second gene, Gdm, which encodes the mitochondrial glycerol 3-phosphate dehydrogenase and has many of the same phenotypes and properties as SLC25A25, including its location in the inner mitochondrial membrane and possession of Ca2+-binding EF-hand domains, will be tested for its role in energy homeostasis. Three specific aims will test the hypothesis that SLC25A25 and GDM function as important components of energy homeostasis in skeletal muscle and heart by maintaining the level of ATP necessary to support Ca2+ cycling across the sarcoplasmic reticulum. In the first aim Slc25a25 will be inactivated selectively in heart and skeletal muscle using the Cre-LoxP system. DIO and physical endurance will be determined as well as fiber type analysis and transcriptome analysis of muscle to determine the effects of Slc25a25 inactivation on expression of genes involved in substrate oxidation and energy metabolism. The second aim will use ex vivo analysis of the perfused heart and skeletal muscle to determine the effects of inactivated Slc25a25 and Gdm on force characteristics of muscle under conditions of altered nutrition. The third aim will utilize mouse embryonic fibroblasts, prepared from mice with inactivated Slc25a25 and Gdm, to investigate the effects of mutant genes on Ca2+ imaging, respiration and ATP production in cells and isolated mitochondria using the Seahorse XF Extracellular Flux analyzer. These studies will establish whether SCL25A25 and GDM support Ca2+ cycling through maintenance of ATP levels and that their inactivation leads to metabolic inefficiency with effects on both muscle physical endurance and adiposity. PUBLIC HEALTH RELEVANCE: The efficiency of energy metabolism, defined as the ability to rapidly produce energy in response to a cellular requirement, is profoundly important for many physiological processes, such as excitation-contraction of heart and skeletal muscle. Consequently, the inefficient production of ATP in the muscle of diabetic patients can lead to cardiovascular disease. Through our studies of mutant mice we have identified a protein called SLC25A25 that is important for the achieving maximal metabolic efficiency, which when inactivated causes extreme muscle fatigue. We found major reductions of Slc25a25 gene expression in skeletal muscle biopsies from obese and type 2 diabetic humans that correlate with insulin sensitivity. In this proposal using genetically engineered mice we will test the hypothesis that SLC25A25 functions by supporting the energy requirements for muscle function and body temperature regulation.

描述(申请人提供)：当能量摄入超过能量消耗时出现的正能量平衡是肥胖发生的基本生理条件。虽然人们对控制食物摄入量的基本机制知之甚少，但除了身体活动外，几乎没有人知道可以被激活以燃烧多余卡路里的生热机制的身份。我们强迫线粒体解偶联蛋白1(UCP1)被去除的小鼠，通过逐渐将它们暴露在寒冷中来激活替代的生热机制。利用几种UCP1缺乏的遗传模型，我们发现在冷应激和饮食诱导肥胖(DIO)的条件下，一个新的基因Slc25a25已经在骨骼肌和腹股沟脂肪中持续诱导。由于Slc25a25编码一个假定的ATP-Mg2+/PI转运体，该转运体位于线粒体内膜上，被认为参与调节线粒体中的ATP水平，因此我们进行了实验，以验证Slc25a25参与能量稳态的假设。SLc25a25基因全局失活的小鼠对DIO具有抵抗力，在跑步机上进行测试时，身体耐力降低。基于SLC25A25具有与钙离子结合的EF-Hand结构域以及其ATP-Mg2+/PI活性受钙调节的事实，我们设计了实验来验证SLC25A25在肌肉兴奋-收缩的最佳能量水平调节中起关键作用的假说。此外，第二个基因GDM编码线粒体3-磷酸甘油脱氢酶，具有许多与SLC25A25相同的表型和特性，包括它位于线粒体内膜和拥有钙结合的EF-Hand结构域，将被测试其在能量平衡中的作用。三个特定的目标将检验这一假设，即SLC25A25和GDM通过维持支持肌浆网中钙循环所需的ATP水平，作为骨骼肌和心脏能量稳态的重要组成部分发挥作用。在第一个目标中，将使用Cre-loxP系统选择性地灭活心肌和骨骼肌中的Slc25a25。将测定肌肉的DIO和身体耐力，以及肌肉的纤维类型分析和转录组分析，以确定Slc25a25失活对底物氧化和能量代谢相关基因表达的影响。第二个目的是通过对灌流的心脏和骨骼肌的体外分析，确定在营养改变的条件下，灭活的SLc25a25和GDM对肌肉力量特征的影响。第三个目标是利用从SLc25a25和GDM灭活小鼠制备的小鼠胚胎成纤维细胞，使用SeaHorse XF细胞外通量分析仪研究突变基因对细胞和分离线粒体中钙成像、呼吸和ATP产生的影响。这些研究将确定SCL25A25和GDM是否通过维持ATP水平来支持钙循环，以及它们的失活是否会导致代谢效率低下，从而影响肌肉的身体耐力和肥胖。 与公共健康相关：能量代谢的效率，被定义为快速产生能量以响应细胞需求的能力，对许多生理过程非常重要，如心脏和骨骼肌的兴奋收缩。因此，糖尿病患者肌肉中ATP的低效产生可能导致心血管疾病。通过对突变小鼠的研究，我们已经确定了一种名为SLC25A25的蛋白质，它对于实现最大的代谢效率很重要，当被灭活时，它会导致极端的肌肉疲劳。我们发现肥胖和2型糖尿病患者骨骼肌活检组织中SLc25a25基因的表达显著降低，这与胰岛素敏感性有关。在这项使用基因工程小鼠的提案中，我们将测试SLC25A25通过支持肌肉功能和体温调节所需的能量来发挥作用的假设。