Ice Free Vitrification and nanowarming of large cartilage samples for transplantation
用于移植的大型软骨样本的无冰玻璃化和纳米加温
基本信息
- 批准号:9473828
- 负责人:
- 金额:$ 15万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-07 至 2019-05-31
- 项目状态:已结题
- 来源:
- 关键词:AllogenicAllograftingAnimal ModelAnimalsBeaversBiocompatible MaterialsBiological AssayBiological PreservationCaliberCardiovascular systemCartilageCell Culture TechniquesCell SurvivalCellsChemistryChondrocytesClinicalClinical ResearchComplexConvectionCouplingCryopreservationDataDefectDegenerative polyarthritisEffectivenessEnsureEvaluationExtracellular MatrixFamily suidaeFood ProcessingFormulationFreedomFreezingFrequenciesFutureHarvestHeatingHumanIACUCIceIn VitroInvestigationIronLifeMagnetismMaintenanceMetabolicMethodsOutcomePatientsPermeabilityPharmacopoeiasPhasePlantsPlug-inPropertyResearchReview LiteratureRewarmingSample SizeSamplingSmall Business Innovation Research GrantSurfaceSurveysTechnologyTestingThickTimeTissue EngineeringTissue SampleTissue ViabilityTissuesTransplantationTraumaTrypan BlueUnited Statesarticular cartilageattenuationbiomaterial compatibilitybonecartilage allograftcartilage repaircommercializationimplantationin vivoinnovationnanoparticleosteochondral tissueparticleradio frequencyreconstructionrepairedresponsetranslation to humans
项目摘要
Resurfacing of articular cartilage with cold stored osteochondral allografts is employed clinically for repair of
trauma and osteoarthritis-induced articular cartilage surface damage. Chondrocyte viability of transplanted
articular cartilage is accepted as one of the determinants of outcome following osteochondral allograft
transplantation. We have previously developed an ice-free vitrification method of cryopreservation that
maintains excellent chondrocyte viability in animal model and human articular cartilage. The chondrocytes
survive vitrification due to the absence of ice formation during cooling and warming of 1-3mL samples.
However, it has not been possible to rewarm larger samples due to ice nucleation during rewarming that
results in loss of chondrocyte viability. The innovation in this proposal relates to a new rewarming method that
does not have the limitations of boundary convection warming that should be effective for samples up to 50mL
in volume. This rewarming method utilizes radio frequency induced heating of magnetic iron nanoparticles. We
will optimize nanowarming of full thickness osteocartilage storage for maintenance of both chondrocyte viability
and extracellular matrix integrity. This objective will be developed in two specific aims to optimize nanowarming
variables and scaleup from 5mL to 30-50mL volumes. Nanowarmed chondrocyte viability, chemistry, and
biomaterial properties will be compared with untreated fresh control tissue. The nanowarming conditions that
provides the best preservation of chondrocytes with minimal if any cartilage biomaterial changes will be
selected for further investigation in vivo and translation to human cartilage in a subsequent Phase II SBIR
application.
冷藏同种异体骨软骨表面置换术在临床上的应用
创伤和骨关节炎所致的关节软骨表面损伤。移植软骨细胞活性的研究
关节软骨被认为是同种异体骨软骨移植结果的决定因素之一。
移植。我们之前已经开发出一种无冰玻璃化冷冻保存方法,
在动物模型和人类关节软骨中保持良好的软骨细胞活性。软骨细胞
在1-3毫升样品的冷却和升温过程中,由于没有结冰,玻璃化保存下来。
然而,由于复温过程中的冰核作用,无法对较大的样品进行复温
导致软骨细胞活力丧失。这项提案的创新涉及一种新的复温方法,
没有边界对流变暖的限制,这种限制对50毫升以下的样品有效
在音量上。这种复温方法利用射频感应加热磁性铁纳米颗粒。我们
将优化全层骨吸收存储的纳米温控,以维持软骨细胞的活力
和细胞外基质的完整性。这一目标将在两个具体目标中发展,以优化纳米武器
变量和规模从5毫升到30-50毫升。纳米级软骨细胞的活性、化学和
生物材料的性能将与未经处理的新鲜对照组织进行比较。纳米武器的条件
提供最好的软骨细胞保存,即使有软骨生物材料的变化也是最小的
选择在体内进行进一步研究并在随后的第二阶段SBIR中移植到人软骨中
申请。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Kelvin G.M. Brockbank其他文献
71. Oxygenated hypothermic machine perfusion improves liver function
- DOI:
10.1016/j.cryobiol.2011.09.074 - 发表时间:
2011-12-01 - 期刊:
- 影响因子:
- 作者:
Kelvin G.M. Brockbank;Charles Y. Lee;Barry J. Fuller;Elizabeth D. Greene;Zhenzhen Chen;Lindsay K. Freeman;Hans R. Kershaw;David Kravitz;Lia H. Campbell - 通讯作者:
Lia H. Campbell
122. Impact of cold ischemia on pancreatic islet cell line viability and apoptosis
- DOI:
10.1016/j.cryobiol.2010.10.126 - 发表时间:
2010-12-01 - 期刊:
- 影响因子:
- 作者:
Lia H. Campbell;Alma Vazquez;Zhenzhen Chen;Michael J. Taylor;Kelvin G.M. Brockbank - 通讯作者:
Kelvin G.M. Brockbank
Vitreous tissue cryopreservation using a blood vessel model and cryomacroscopy for scale-up studies: Observations and mathematical modeling
- DOI:
10.1016/j.cryobiol.2024.104976 - 发表时间:
2024-12-01 - 期刊:
- 影响因子:
- 作者:
Michael J. Taylor;Prem K. Solanki;Zhenzhen Chen;Simona Baicu;Christina Crossley;Elizabeth D. Greene;Lia H. Campbell;Kelvin G.M. Brockbank;Yoed Rabin - 通讯作者:
Yoed Rabin
70. Comparison of liver hypothermic machine perfusion at 4–6 and 12–14 °C
- DOI:
10.1016/j.cryobiol.2010.10.074 - 发表时间:
2010-12-01 - 期刊:
- 影响因子:
- 作者:
Kelvin G.M. Brockbank;Charles Y. Lee;Elizabeth D. Greene;Zhenzhen Chen;Lindsay K. Freeman;Simona C. Baicu;David Kravitz;Lia H. Campbell - 通讯作者:
Lia H. Campbell
Optimization of hypothermic cartilage storage for chondrocyte viability and biomaterial preservation.
- DOI:
10.1016/j.cryobiol.2018.10.106 - 发表时间:
2018-12-01 - 期刊:
- 影响因子:
- 作者:
Kelvin G.M. Brockbank;Glenn Hepfer;Greg J. Wright;Lia H. Campbell;Zhen Chen;Elizabeth D. Greene;Hai Yao - 通讯作者:
Hai Yao
Kelvin G.M. Brockbank的其他文献
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{{ truncateString('Kelvin G.M. Brockbank', 18)}}的其他基金
Ice-free vitrification and nanowarming of meniscal grafts for transplantation
用于移植的半月板移植物的无冰玻璃化和纳米加温
- 批准号:
10819333 - 财政年份:2023
- 资助金额:
$ 15万 - 项目类别:
Mechanistic approach to optimization of a kidney preservation solution
优化肾脏保存溶液的机械方法
- 批准号:
10545982 - 财政年份:2022
- 资助金额:
$ 15万 - 项目类别:
Extended limb preservation employing an optimization strategy for stabilization.
采用优化稳定策略来延长肢体保护。
- 批准号:
10257524 - 财政年份:2021
- 资助金额:
$ 15万 - 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
- 批准号:
10379220 - 财政年份:2020
- 资助金额:
$ 15万 - 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
- 批准号:
10026454 - 财政年份:2020
- 资助金额:
$ 15万 - 项目类别:
Ice-free cryopreservation of whole pediatric testes for autologous banking and replantation.
整个儿科睾丸的无冰冷冻保存用于自体储存和再植。
- 批准号:
9919065 - 财政年份:2020
- 资助金额:
$ 15万 - 项目类别:
Feasibility of expanding ischemia time for hearts destined for transplantation
延长移植心脏缺血时间的可行性
- 批准号:
10082625 - 财政年份:2020
- 资助金额:
$ 15万 - 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
- 批准号:
10587348 - 财政年份:2020
- 资助金额:
$ 15万 - 项目类别:
Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation
用于移植的大型骨软骨移植物的无冰玻璃化和纳米加温
- 批准号:
9918800 - 财政年份:2017
- 资助金额:
$ 15万 - 项目类别:
Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation
用于移植的大型骨软骨移植物的无冰玻璃化和纳米加温
- 批准号:
10017008 - 财政年份:2017
- 资助金额:
$ 15万 - 项目类别:
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