Ice Free Vitrification and nanowarming of large cartilage samples for transplantation

用于移植的大型软骨样本的无冰玻璃化和纳米加温

基本信息

项目摘要

Resurfacing of articular cartilage with cold stored osteochondral allografts is employed clinically for repair of trauma and osteoarthritis-induced articular cartilage surface damage. Chondrocyte viability of transplanted articular cartilage is accepted as one of the determinants of outcome following osteochondral allograft transplantation. We have previously developed an ice-free vitrification method of cryopreservation that maintains excellent chondrocyte viability in animal model and human articular cartilage. The chondrocytes survive vitrification due to the absence of ice formation during cooling and warming of 1-3mL samples. However, it has not been possible to rewarm larger samples due to ice nucleation during rewarming that results in loss of chondrocyte viability. The innovation in this proposal relates to a new rewarming method that does not have the limitations of boundary convection warming that should be effective for samples up to 50mL in volume. This rewarming method utilizes radio frequency induced heating of magnetic iron nanoparticles. We will optimize nanowarming of full thickness osteocartilage storage for maintenance of both chondrocyte viability and extracellular matrix integrity. This objective will be developed in two specific aims to optimize nanowarming variables and scaleup from 5mL to 30-50mL volumes. Nanowarmed chondrocyte viability, chemistry, and biomaterial properties will be compared with untreated fresh control tissue. The nanowarming conditions that provides the best preservation of chondrocytes with minimal if any cartilage biomaterial changes will be selected for further investigation in vivo and translation to human cartilage in a subsequent Phase II SBIR application.
临床上采用冷保存的同种异体骨软骨移植物进行关节软骨表面置换, 创伤和骨关节炎引起的关节软骨表面损伤。移植软骨细胞活性 关节软骨被认为是骨软骨移植后结果的决定因素之一 移植我们以前已经开发了一种无冰玻璃化冷冻保存方法, 在动物模型和人关节软骨中保持优异的软骨细胞活力。软骨细胞 由于在1 - 3mL样品的冷却和加热期间没有冰形成,因此能够在玻璃化中存活。 然而,由于复温过程中的冰成核, 导致软骨细胞活力丧失。该提案的创新涉及一种新的复温方法, 不存在边界对流加热的局限性,边界对流加热应对高达50 mL的样品有效 在数量上。这种复温方法利用磁性铁纳米颗粒的射频诱导加热。我们 将优化全层骨软骨储存的预处理, 和细胞外基质的完整性。这一目标将分为两个具体目标, 变量和规模从5 mL到30 - 50 mL体积。纳米武装软骨细胞的活力,化学,和 将生物材料性能与未处理的新鲜对照组织进行比较。这些条件是 提供了软骨细胞的最佳保存, 选择用于进一步的体内研究,并在随后的II期SBIR中转化为人软骨 应用程序.

项目成果

期刊论文数量(0)
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Kelvin G.M. Brockbank其他文献

71. Oxygenated hypothermic machine perfusion improves liver function
  • DOI:
    10.1016/j.cryobiol.2011.09.074
  • 发表时间:
    2011-12-01
  • 期刊:
  • 影响因子:
  • 作者:
    Kelvin G.M. Brockbank;Charles Y. Lee;Barry J. Fuller;Elizabeth D. Greene;Zhenzhen Chen;Lindsay K. Freeman;Hans R. Kershaw;David Kravitz;Lia H. Campbell
  • 通讯作者:
    Lia H. Campbell
122. Impact of cold ischemia on pancreatic islet cell line viability and apoptosis
  • DOI:
    10.1016/j.cryobiol.2010.10.126
  • 发表时间:
    2010-12-01
  • 期刊:
  • 影响因子:
  • 作者:
    Lia H. Campbell;Alma Vazquez;Zhenzhen Chen;Michael J. Taylor;Kelvin G.M. Brockbank
  • 通讯作者:
    Kelvin G.M. Brockbank
Vitreous tissue cryopreservation using a blood vessel model and cryomacroscopy for scale-up studies: Observations and mathematical modeling
  • DOI:
    10.1016/j.cryobiol.2024.104976
  • 发表时间:
    2024-12-01
  • 期刊:
  • 影响因子:
  • 作者:
    Michael J. Taylor;Prem K. Solanki;Zhenzhen Chen;Simona Baicu;Christina Crossley;Elizabeth D. Greene;Lia H. Campbell;Kelvin G.M. Brockbank;Yoed Rabin
  • 通讯作者:
    Yoed Rabin
70. Comparison of liver hypothermic machine perfusion at 4–6 and 12–14 °C
  • DOI:
    10.1016/j.cryobiol.2010.10.074
  • 发表时间:
    2010-12-01
  • 期刊:
  • 影响因子:
  • 作者:
    Kelvin G.M. Brockbank;Charles Y. Lee;Elizabeth D. Greene;Zhenzhen Chen;Lindsay K. Freeman;Simona C. Baicu;David Kravitz;Lia H. Campbell
  • 通讯作者:
    Lia H. Campbell
Optimization of hypothermic cartilage storage for chondrocyte viability and biomaterial preservation.
  • DOI:
    10.1016/j.cryobiol.2018.10.106
  • 发表时间:
    2018-12-01
  • 期刊:
  • 影响因子:
  • 作者:
    Kelvin G.M. Brockbank;Glenn Hepfer;Greg J. Wright;Lia H. Campbell;Zhen Chen;Elizabeth D. Greene;Hai Yao
  • 通讯作者:
    Hai Yao

Kelvin G.M. Brockbank的其他文献

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{{ truncateString('Kelvin G.M. Brockbank', 18)}}的其他基金

Ice-free vitrification and nanowarming of meniscal grafts for transplantation
用于移植的半月板移植物的无冰玻璃化和纳米加温
  • 批准号:
    10819333
  • 财政年份:
    2023
  • 资助金额:
    $ 15万
  • 项目类别:
Mechanistic approach to optimization of a kidney preservation solution
优化肾脏保存溶液的机械方法
  • 批准号:
    10545982
  • 财政年份:
    2022
  • 资助金额:
    $ 15万
  • 项目类别:
Extended limb preservation employing an optimization strategy for stabilization.
采用优化稳定策略来延长肢体保护。
  • 批准号:
    10257524
  • 财政年份:
    2021
  • 资助金额:
    $ 15万
  • 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
  • 批准号:
    10379220
  • 财政年份:
    2020
  • 资助金额:
    $ 15万
  • 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
  • 批准号:
    10026454
  • 财政年份:
    2020
  • 资助金额:
    $ 15万
  • 项目类别:
Ice-free cryopreservation of whole pediatric testes for autologous banking and replantation.
整个儿科睾丸的无冰冷冻保存用于自体储存和再植。
  • 批准号:
    9919065
  • 财政年份:
    2020
  • 资助金额:
    $ 15万
  • 项目类别:
Feasibility of expanding ischemia time for hearts destined for transplantation
延长移植心脏缺血时间的可行性
  • 批准号:
    10082625
  • 财政年份:
    2020
  • 资助金额:
    $ 15万
  • 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
  • 批准号:
    10587348
  • 财政年份:
    2020
  • 资助金额:
    $ 15万
  • 项目类别:
Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation
用于移植的大型骨软骨移植物的无冰玻璃化和纳米加温
  • 批准号:
    9918800
  • 财政年份:
    2017
  • 资助金额:
    $ 15万
  • 项目类别:
Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation
用于移植的大型骨软骨移植物的无冰玻璃化和纳米加温
  • 批准号:
    10017008
  • 财政年份:
    2017
  • 资助金额:
    $ 15万
  • 项目类别:

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Establishment of novel osteochondral allografting combined with growth factor- collagen-binding domain fusion technology
新型同种异体骨软骨移植联合生长因子-胶原蛋白结合域融合技术的建立
  • 批准号:
    26462277
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Translating PTH Therapy as an Adjuvant for Structural Allografting
将 PTH 疗法转化为结构性同种异体移植的佐剂
  • 批准号:
    8344380
  • 财政年份:
    2012
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    $ 15万
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Allografting for Lukemia
白血病同种异体移植
  • 批准号:
    8260361
  • 财政年份:
    2011
  • 资助金额:
    $ 15万
  • 项目类别:
Composite Allografting for Promoting Survival of Corneal Transplants
复合同种异体移植促进角膜移植的存活
  • 批准号:
    7878675
  • 财政年份:
    2009
  • 资助金额:
    $ 15万
  • 项目类别:
Composite Allografting for Promoting Survival of Corneal Transplants
复合同种异体移植促进角膜移植的存活
  • 批准号:
    7677758
  • 财政年份:
    2009
  • 资助金额:
    $ 15万
  • 项目类别:
Augmenting Antitumor Immunity after Allografting
增强同种异体移植后的抗肿瘤免疫力
  • 批准号:
    7466112
  • 财政年份:
    2008
  • 资助金额:
    $ 15万
  • 项目类别:
Augmenting Antitumor Immunity after Allografting
增强同种异体移植后的抗肿瘤免疫力
  • 批准号:
    8010394
  • 财政年份:
    2008
  • 资助金额:
    $ 15万
  • 项目类别:
Augmenting Antitumor Immunity after Allografting
增强同种异体移植后的抗肿瘤免疫力
  • 批准号:
    8208131
  • 财政年份:
    2008
  • 资助金额:
    $ 15万
  • 项目类别:
Augmenting Antitumor Immunity after Allografting
增强同种异体移植后的抗肿瘤免疫力
  • 批准号:
    7575273
  • 财政年份:
    2008
  • 资助金额:
    $ 15万
  • 项目类别:
Augmenting Antitumor Immunity after Allografting
增强同种异体移植后的抗肿瘤免疫力
  • 批准号:
    7765518
  • 财政年份:
    2008
  • 资助金额:
    $ 15万
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