Ice-free vitrification and nanowarming of meniscal grafts for transplantation
用于移植的半月板移植物的无冰玻璃化和纳米加温
基本信息
- 批准号:10819333
- 负责人:
- 金额:$ 30.2万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-20 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AlamarBlueAllogenicAllograftingAnimal ModelAnimalsBiocompatible MaterialsBiological AssayBiomechanicsCardiovascular systemCartilageCell Culture TechniquesCell MaintenanceCell SurvivalCellsChemistryChondrocytesConvectionCouplingCryopreservationCrystallizationDataDiameterDimethyl SulfoxideEffectivenessEvaluationExtracellular MatrixFamily suidaeFibrocartilagesFood ProcessingFormulationFreezingFrequenciesFutureGene ExpressionGene Expression ProfilingHarvestHeart ValvesHeatingHumanIACUCIceIn VitroInvestigationIronIschemiaKneeLateral meniscus structureLifeMagnetic nanoparticlesMagnetismMedial meniscus structureMeniscus structure of jointMetabolicMethodsOperative Surgical ProceduresOutcomePatient-Focused OutcomesPatientsPenetrationPermeabilityPharmacopoeiasPhasePlantsPropertyPublicationsResearchReview LiteratureRewarmingSample SizeSamplingSideSmall Business Innovation Research GrantStainsSurveysTechnologyTestingThickThinnessTissue EngineeringTissue SampleTissue ViabilityTissuesTransplantationTrypan BlueUnited Statesarticular cartilageattenuationbiomaterial compatibilityclinical applicationcommercializationexperimental groupin vivoinnovationnanoparticlenanowarmingparticlepreservationradio frequencyreconstructionrepairedtranslation to humans
项目摘要
ABSTRACT: Limited availability of fresh viable donor meniscal grafts reduces size-matching efficiency for
meniscal allograft transplantation, restricting surgical accessibility and patient outcomes. Current preservation
methods with retention of meniscal viability are inadequate. Fibrochondrocyte viability of transplanted menisci is
accepted as one of the determinants of outcome following allograft transplantation. We have previously
developed an ice-free vitrification method of cryopreservation that maintains excellent chondrocyte viability in
animal models and human articular cartilage. We present preliminary data here demonstrating that
fibrochondrocytes in menisci can also survive vitrification due to the absence of ice formation during cooling and
warming of small 1-3mL samples. However, it has not been possible to rewarm larger samples due to ice
nucleation during rewarming that results in loss of cell viability. The innovation in this proposal relates to a new
rewarming method that does not have the limitations of boundary convection warming that should be effective
for samples up to 50mL in volume. This rewarming method utilizes radio frequency induced heating of magnetic
iron nanoparticles. We will optimize nanowarming of full thickness meniscal fibrocartilage plugs for maintenance
of cell viability, including gene expression, and extracellular matrix integrity. This objective will be developed in
two specific aims to optimize nanowarming variables and scaleup from 5mL employing tissue plugs to 30mL with
intact menisci. Menisci will be vitrified and stored for 1-4 weeks and then nanowarmed meniscal cell viability,
chemistry, and biomaterial properties will be compared with untreated fresh control tissue. The nanowarming
conditions that provides the best preservation of cells with minimal if any biomaterial changes will be selected
for further investigation in vivo and translation to human menisci in a subsequent, future, Phase II SBIR
application.
摘要:新鲜活体半月板移植物的可获得性有限降低了手术的大小匹配效率
同种异体半月板移植,限制了手术的可及性和患者的预后。当前保存
保留半月板存活的方法是不够的。半月板移植后纤维软骨细胞活性的研究
被认为是同种异体移植后结果的决定因素之一。我们之前已经
开发了一种无冰玻璃化冷冻保存方法,该方法可保持良好的软骨细胞活力
动物模型和人类关节软骨。我们在这里提供的初步数据表明
半月板中的纤维软骨细胞也可以在玻璃化冷冻中存活下来,因为在冷却和冷冻过程中没有结冰
将1-3毫升的小样品加热。然而,由于结冰,无法对更大的样品进行重新加热
在复温过程中导致细胞活力丧失的核化。这项提案的创新之处在于一项新的
不存在边界对流增温应有效的局限性的复温方法
适用于体积不超过50毫升的样品。这种复温方法利用射频感应加热磁场
铁纳米粒子。我们将优化全厚度半月板纤维软骨塞的纳米温控以进行维护
细胞活力,包括基因表达,以及细胞外基质的完整性。这一目标将在#年制定
两个具体的目标是优化纳米温控变量,并使用组织塞从5ml扩大到30ml
半月板完好无损。半月板将被玻璃化保存1-4周,然后纳米臂半月板细胞存活,
化学和生物材料特性将与未经处理的新鲜对照组织进行比较。纳米武器
提供最好的细胞保存的条件,如果有任何生物材料的变化,最小的变化将被选择
用于进一步的活体研究和在未来的第二阶段SBIR中移植到人半月板上
申请。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Kelvin G.M. Brockbank其他文献
71. Oxygenated hypothermic machine perfusion improves liver function
- DOI:
10.1016/j.cryobiol.2011.09.074 - 发表时间:
2011-12-01 - 期刊:
- 影响因子:
- 作者:
Kelvin G.M. Brockbank;Charles Y. Lee;Barry J. Fuller;Elizabeth D. Greene;Zhenzhen Chen;Lindsay K. Freeman;Hans R. Kershaw;David Kravitz;Lia H. Campbell - 通讯作者:
Lia H. Campbell
122. Impact of cold ischemia on pancreatic islet cell line viability and apoptosis
- DOI:
10.1016/j.cryobiol.2010.10.126 - 发表时间:
2010-12-01 - 期刊:
- 影响因子:
- 作者:
Lia H. Campbell;Alma Vazquez;Zhenzhen Chen;Michael J. Taylor;Kelvin G.M. Brockbank - 通讯作者:
Kelvin G.M. Brockbank
Vitreous tissue cryopreservation using a blood vessel model and cryomacroscopy for scale-up studies: Observations and mathematical modeling
- DOI:
10.1016/j.cryobiol.2024.104976 - 发表时间:
2024-12-01 - 期刊:
- 影响因子:
- 作者:
Michael J. Taylor;Prem K. Solanki;Zhenzhen Chen;Simona Baicu;Christina Crossley;Elizabeth D. Greene;Lia H. Campbell;Kelvin G.M. Brockbank;Yoed Rabin - 通讯作者:
Yoed Rabin
70. Comparison of liver hypothermic machine perfusion at 4–6 and 12–14 °C
- DOI:
10.1016/j.cryobiol.2010.10.074 - 发表时间:
2010-12-01 - 期刊:
- 影响因子:
- 作者:
Kelvin G.M. Brockbank;Charles Y. Lee;Elizabeth D. Greene;Zhenzhen Chen;Lindsay K. Freeman;Simona C. Baicu;David Kravitz;Lia H. Campbell - 通讯作者:
Lia H. Campbell
Optimization of hypothermic cartilage storage for chondrocyte viability and biomaterial preservation.
- DOI:
10.1016/j.cryobiol.2018.10.106 - 发表时间:
2018-12-01 - 期刊:
- 影响因子:
- 作者:
Kelvin G.M. Brockbank;Glenn Hepfer;Greg J. Wright;Lia H. Campbell;Zhen Chen;Elizabeth D. Greene;Hai Yao - 通讯作者:
Hai Yao
Kelvin G.M. Brockbank的其他文献
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{{ truncateString('Kelvin G.M. Brockbank', 18)}}的其他基金
Mechanistic approach to optimization of a kidney preservation solution
优化肾脏保存溶液的机械方法
- 批准号:
10545982 - 财政年份:2022
- 资助金额:
$ 30.2万 - 项目类别:
Extended limb preservation employing an optimization strategy for stabilization.
采用优化稳定策略来延长肢体保护。
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10257524 - 财政年份:2021
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$ 30.2万 - 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
- 批准号:
10379220 - 财政年份:2020
- 资助金额:
$ 30.2万 - 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
- 批准号:
10026454 - 财政年份:2020
- 资助金额:
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Ice-free cryopreservation of whole pediatric testes for autologous banking and replantation.
整个儿科睾丸的无冰冷冻保存用于自体储存和再植。
- 批准号:
9919065 - 财政年份:2020
- 资助金额:
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Feasibility of expanding ischemia time for hearts destined for transplantation
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- 批准号:
10082625 - 财政年份:2020
- 资助金额:
$ 30.2万 - 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
- 批准号:
10587348 - 财政年份:2020
- 资助金额:
$ 30.2万 - 项目类别:
Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation
用于移植的大型骨软骨移植物的无冰玻璃化和纳米加温
- 批准号:
9918800 - 财政年份:2017
- 资助金额:
$ 30.2万 - 项目类别:
Ice Free Vitrification and nanowarming of large cartilage samples for transplantation
用于移植的大型软骨样本的无冰玻璃化和纳米加温
- 批准号:
9473828 - 财政年份:2017
- 资助金额:
$ 30.2万 - 项目类别:
Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation
用于移植的大型骨软骨移植物的无冰玻璃化和纳米加温
- 批准号:
10017008 - 财政年份:2017
- 资助金额:
$ 30.2万 - 项目类别:
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