Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation
用于移植的大型骨软骨移植物的无冰玻璃化和纳米加温
基本信息
- 批准号:10017008
- 负责人:
- 金额:$ 82.67万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-07 至 2023-08-31
- 项目状态:已结题
- 来源:
- 关键词:AllogenicAllograftingAnimal ModelBeaversBiocompatible MaterialsCardiovascular systemCartilageCell SurvivalCellsChemistryChondrocytesClinicalClinical ResearchContralateralConvectionCouplingCryopreservationCustomDataDefectDegenerative polyarthritisDevelopmentEffectivenessEnsureEvaluationExtracellular MatrixFamily suidaeFarming environmentFood ProcessingFormulationFreedomFreezingFrequenciesFutureHeatingHistologyHumanIACUCIceImplantIn VitroInvestigationIronLeadMagnetismMaintenanceMetabolicMethodsMiniature SwineModelingOutcomePatientsPermeabilityPhasePlantsPropertyRecoveryResearchReview LiteratureRewarmingSample SizeSamplingSmall Business Innovation Research GrantSurfaceSurveysTechnologyTestingThickTimeTissue EngineeringTissue ViabilityTissuesTransplantationTraumaarticular cartilageattenuationbiomaterial compatibilitycartilage cellcommercializationimplantationimprovedin vivoin vivo evaluationinnovationnanoparticlenanowarmingosteochondral tissueparticlephase 1 studypreclinical studypreservationradio frequencyreconstructionrepairedresponsesuccesstranslation to humanstransplant model
项目摘要
Resurfacing of articular cartilage with cold stored osteochondral allografts is employed clinically for repair of
trauma and osteoarthritis-induced articular cartilage surface damage. Chondrocyte viability of transplanted
articular cartilage is accepted as one of the determinants of outcome following osteochondral allograft
transplantation. We have previously developed an ice-free vitrification method of cryopreservation that
maintains excellent chondrocyte viability in animal model and human articular cartilage. The chondrocytes
survive vitrification due to the absence of ice formation during cooling and warming of 1-3mL samples.
However, it had not been possible to rewarm larger samples due to ice nucleation during rewarming that
results in loss of chondrocyte viability prior to our Phase I studies. The innovation in this proposal relates to a
new rewarming method that does not have the limitations of boundary convection warming that should be
effective for samples up to 50mL in volume. This rewarming method utilizes radio frequency induced heating of
magnetic iron nanoparticles. In Phase I we demonstrated the effectiveness of ice-free vitrification combined
with nanowarming for large full thickness osteochondral tissues in 50 mL volumes in 80 seconds with
maintenance of both chondrocyte viability and extracellular matrix integrity. In Phase II we propose
development of a porcine model, further optimization of ice-free vitrification and nanowarming and in vivo
evaluation in a porcine model in three specific aims. Nanowarmed chondrocyte viability, chemistry, and
biomaterial properties will be compared with untreated fresh control tissues both before and after transplant.
The nanowarming conditions that provides the best preservation of chondrocytes with minimal if any cartilage
biomaterial changes will be selected for further investigation and translation to human cartilage in a
subsequent Phase IIb SBIR application.
骨软骨冷库异体移植关节软骨表面修复是临床上常用的修复方法
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nanowarming and ice-free cryopreservation of large sized, intact porcine articular cartilage.
- DOI:10.1038/s42003-023-04577-9
- 发表时间:2023-02-24
- 期刊:
- 影响因子:5.9
- 作者:
- 通讯作者:
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Kelvin G.M. Brockbank其他文献
71. Oxygenated hypothermic machine perfusion improves liver function
- DOI:
10.1016/j.cryobiol.2011.09.074 - 发表时间:
2011-12-01 - 期刊:
- 影响因子:
- 作者:
Kelvin G.M. Brockbank;Charles Y. Lee;Barry J. Fuller;Elizabeth D. Greene;Zhenzhen Chen;Lindsay K. Freeman;Hans R. Kershaw;David Kravitz;Lia H. Campbell - 通讯作者:
Lia H. Campbell
122. Impact of cold ischemia on pancreatic islet cell line viability and apoptosis
- DOI:
10.1016/j.cryobiol.2010.10.126 - 发表时间:
2010-12-01 - 期刊:
- 影响因子:
- 作者:
Lia H. Campbell;Alma Vazquez;Zhenzhen Chen;Michael J. Taylor;Kelvin G.M. Brockbank - 通讯作者:
Kelvin G.M. Brockbank
Vitreous tissue cryopreservation using a blood vessel model and cryomacroscopy for scale-up studies: Observations and mathematical modeling
- DOI:
10.1016/j.cryobiol.2024.104976 - 发表时间:
2024-12-01 - 期刊:
- 影响因子:
- 作者:
Michael J. Taylor;Prem K. Solanki;Zhenzhen Chen;Simona Baicu;Christina Crossley;Elizabeth D. Greene;Lia H. Campbell;Kelvin G.M. Brockbank;Yoed Rabin - 通讯作者:
Yoed Rabin
70. Comparison of liver hypothermic machine perfusion at 4–6 and 12–14 °C
- DOI:
10.1016/j.cryobiol.2010.10.074 - 发表时间:
2010-12-01 - 期刊:
- 影响因子:
- 作者:
Kelvin G.M. Brockbank;Charles Y. Lee;Elizabeth D. Greene;Zhenzhen Chen;Lindsay K. Freeman;Simona C. Baicu;David Kravitz;Lia H. Campbell - 通讯作者:
Lia H. Campbell
Optimization of hypothermic cartilage storage for chondrocyte viability and biomaterial preservation.
- DOI:
10.1016/j.cryobiol.2018.10.106 - 发表时间:
2018-12-01 - 期刊:
- 影响因子:
- 作者:
Kelvin G.M. Brockbank;Glenn Hepfer;Greg J. Wright;Lia H. Campbell;Zhen Chen;Elizabeth D. Greene;Hai Yao - 通讯作者:
Hai Yao
Kelvin G.M. Brockbank的其他文献
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{{ truncateString('Kelvin G.M. Brockbank', 18)}}的其他基金
Ice-free vitrification and nanowarming of meniscal grafts for transplantation
用于移植的半月板移植物的无冰玻璃化和纳米加温
- 批准号:
10819333 - 财政年份:2023
- 资助金额:
$ 82.67万 - 项目类别:
Mechanistic approach to optimization of a kidney preservation solution
优化肾脏保存溶液的机械方法
- 批准号:
10545982 - 财政年份:2022
- 资助金额:
$ 82.67万 - 项目类别:
Extended limb preservation employing an optimization strategy for stabilization.
采用优化稳定策略来延长肢体保护。
- 批准号:
10257524 - 财政年份:2021
- 资助金额:
$ 82.67万 - 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
- 批准号:
10379220 - 财政年份:2020
- 资助金额:
$ 82.67万 - 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
- 批准号:
10026454 - 财政年份:2020
- 资助金额:
$ 82.67万 - 项目类别:
Ice-free cryopreservation of whole pediatric testes for autologous banking and replantation.
整个儿科睾丸的无冰冷冻保存用于自体储存和再植。
- 批准号:
9919065 - 财政年份:2020
- 资助金额:
$ 82.67万 - 项目类别:
Feasibility of expanding ischemia time for hearts destined for transplantation
延长移植心脏缺血时间的可行性
- 批准号:
10082625 - 财政年份:2020
- 资助金额:
$ 82.67万 - 项目类别:
Ice-free vitrification and nano warming technology for banking of cardiovascular structures.
用于心血管结构银行的无冰玻璃化和纳米加温技术。
- 批准号:
10587348 - 财政年份:2020
- 资助金额:
$ 82.67万 - 项目类别:
Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation
用于移植的大型骨软骨移植物的无冰玻璃化和纳米加温
- 批准号:
9918800 - 财政年份:2017
- 资助金额:
$ 82.67万 - 项目类别:
Ice Free Vitrification and nanowarming of large cartilage samples for transplantation
用于移植的大型软骨样本的无冰玻璃化和纳米加温
- 批准号:
9473828 - 财政年份:2017
- 资助金额:
$ 82.67万 - 项目类别:
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