Light responses and microcircuitry of two novel amacrine cell types in the retina

视网膜中两种新型无长突细胞类型的光响应和微电路

• 批准号: 9461662
• 负责人: Jason Jacoby
• 金额: $ 0.06万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9461662
• 关键词: Address; Afferent Neurons; Amacrine Cells; Biological Neural Networks; Brain; CRF receptor type 1; Cells; Clinical; Complex; Coupled; Coupling; Development; Diagnostic tests; Dopamine; Feedback; Gap Junctions; Goals; Interneurons; Intervention; Knowledge; Label; Lateral; Left; Light; Measurement; Measures; Mediating; Morphology; Mus; Nervous system structure; Neuraxis; Neurodegenerative Disorders; Neurons; Neurophysiology - biologic function; Neurosciences; Nitric Oxide; Physiological; Physiology; Play; Property; Research; Retina; Retinal; Retinal Diseases; Retinal Ganglion Cells; Role; Signal Pathway; Structure; Synapses; System; Tissues; Typology; Visual; cell type; combat; experimental study; frontier; horizontal cell; information processing; insight; luminance; neural circuit; novel; novel therapeutics; postsynaptic; public health relevance; receptive field; relating to nervous system; response; signal processing; visual processing; visual stimulus

 DESCRIPTION (provided by applicant): Despite decades of research characterizing the structure and function of numerous cell types in the retina, the encoding properties of retinal amacrine cells remain largely unknown. The overall goal of my research is to contribute to our current understanding of visual processing in the retina by characterizing the intrinsic properties and connectivity of two newly identified retinal amacrine cell subtypes. To determine the physiological properties and synaptic microcircuitry of the CRH-1 retinal amacrine cell. I will be the first to (A) characterize the light responses of genetically labeled CRH-1 amacrine cells to a variety of visual stimuli. I will then (B) place CRH-1 into the context of a functional circuit by identifying postsynaptic retinal ganglion cells. These measurements will allow me determine whether CRH-1 cells could mediate feedforward inhibition in the inner retina. To examine the dynamics of gap-junctional coupling between nNOS-2 amacrine cells and the role of this network in visual processing. In preliminary experiments characterizing another genetically labeled amacrine cell, nNOS-2, I have discovered that these cells form an extensive coupled network. I will examine (A) whether nNOS-2 coupling is modulated by mean luminance and whether the modulatory signaling pathway involves dopamine or nitric oxide. I will also (B) determine how nNOS-2 coupling impacts visual response properties by measuring dendritic morphology and receptive fields in the same cells.