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Measuring multiprotein assemblies that drive biological signals

测量驱动生物信号的多蛋白组装体

基本信息

批准号：
9242653
负责人：
Adam G. Schrum
金额：
$ 4.16万
依托单位：
MAYO CLINIC ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-04-01 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9242653
关键词：
Adopted Alopecia Areata Autoimmune Diseases Award Binding Biochemical Biochemical Process Biocompatible Materials Bioinformatics Biological Biological Assay Biology Biometry Cells Chimeric Proteins Clinical Co-Immunoprecipitations Collection Complex Data Data Analyses Data Set Dimerization Disease Drug Targeting Electronic Mail Epitopes Flow Cytometry Generations Grant Health Homo Human Human Engineering Imagery Immune In Vitro Maps Measurement Measures Methodology Methods Microspheres Mouse Protein Mus Pathway interactions Patients Physiological Predictive Value Principal Investigator Protein Analysis Proteins Publishing Sample Size Sampling Signal Transduction Signaling Protein Source Surface Systems Analysis T-Lymphocyte Therapeutic Yeasts base biosignature clinical practice clinically relevant design dimer experimental study field study innovation interest monomer novel outcome forecast protein complex protein protein interaction public health relevance styrofoam treatment response virtual yeast two hybrid system

项目摘要

Grant Number: 1R01GM103841-01A1 Principal Investigator(s): Adam G. Schrum, PHD Project Title: Measuring multiprotein assemblies that drive biological signals Award e-mailed to: researchadmin@mayo.edu Project Period: 04/01/2013 – 03/31/2018 Current Date: April 15, 2015 Modified Project Summary/Abstract Section Proteins interact with each other to form complexes, and these complexes can be dynamic and interchanging as they relay biological signals. However, despite their central importance to biology and disease, protein complexes can be difficult to visualize and assess. There are even more technological barriers to analyzing protein complexes when they originate from non-genetically engineered human cells, such as those that would be provided in clinical patient samples. Currently, because physiologic protein complex profiles are virtually unobtainable, clinical practice cannot use them to assist in human health endeavors. We propose to advance a new strategy by mounting a new assay platform, MIF, to bring the analysis of physiologic, human protein complex profiles online. We have already learned how to overcome and control for the technical hurdles, leading us to launch MIF by targeting 21 human proteins that can participate in 231 inter-protein associations (Specific Aim 1). MIF will allow for analysis of small samples and high-throughput formatting, favoring its adoptability for primary human samples originating from clinical patients. Data analysis will involve the generation of Bioinformatics strategies (Specific Aim 2) to focus on multiplicity of proteins in shared complexes detected by exposed surface epitopes, to assess network protein associations. We will field-test MIF by applying it to the analysis of human protein complexes that may be associated with the autoimmune disease, Alopecia Areata (Specific Aim 3). Together, MIF and its unique analysis will make available the acquisition of physiologic, human complex profiles. We propose that the patient-derived MIF data will exemplify a new strategy for analyzing these complexes, and illustrate its general applicability to many fields of study and classes of disease.

资助号：1R01GM103841-01A1