Multiplex assay of T cell protein complexes for high-throughput drug screening

用于高通量药物筛选的 T 细胞蛋白复合物多重测定

• 批准号: 8077087
• 负责人: Adam G. Schrum
• 金额: $ 39.38万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-14 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8077087
• 关键词: Affect; Antigens; Autoimmune Diseases; Autoimmunity; Biochemical; Biological Assay; Cell Death; Cell physiology; Cells; Chemicals; Clinical; Clonal Expansion; Collaborations; Collection; Communicable Diseases; Complex; Detection; Development; Disease; Epitopes; Equilibrium; Evaluation; Flow Cytometry; Generations; Genetic Engineering; Goals; Graft Rejection; Human; Immune System Diseases; Immune response; Immune system; Immunity; Immunoprecipitation; Laboratories; Lead; Libraries; Malignant Neoplasms; Measurable; Measurement; Measures; Mediating; Medicine; Memory; Methods; Minnesota; Molecular; Molecular Bank; Monitor; Multiprotein Complexes; Mus; Organ Transplantation; Pathway interactions; Pharmaceutical Preparations; Pharmacotherapy; Physiological; Plant Roots; Population; Preclinical Drug Evaluation; Property; Proteins; Radioactivity; Research; Resources; Sampling; Screening procedure; Signal Transduction; Source; T cell response; T-Cell Activation; T-Cell Receptor; T-Lymphocyte; Techniques; Technology; Testing; Therapeutic; Time; United States National Institutes of Health; Universities; Wild Type Mouse; Work; abstracting; anergy; base; combat; design; disorder control; drug candidate; drug efficacy; empowered; field study; high throughput screening; improved; in vivo; interest; prevent; protein complex; protein protein interaction; response

项目成果

Multiplex IP-FCM (immunoprecipitation-flow cytometry): Principles and guidelines for assessing physiologic protein-protein interactions in multiprotein complexes.

• DOI: 10.1016/j.ymeth.2011.09.005
• 发表时间: 2012-02
• 期刊: METHODS
• 影响因子: 4.8
• 作者: Bida, Anya T.;Gil, Diana;Schrum, Adam G.
• 通讯作者: Schrum, Adam G.

Robustness and Specificity in Signal Transduction via Physiologic Protein Interaction Networks.

• DOI: 10.4172/2161-1459.s3-001
• 发表时间: 2012-12-21
• 期刊: Clinical & experimental pharmacology
• 作者: Schrum AG;Gil D
• 通讯作者: Gil D

Visualization of multiprotein complexes by flow cytometry.

• DOI: 10.1002/0471142735.im0509s87
• 发表时间: 2009-11
• 期刊: Current protocols in immunology
• 作者: Schrum, Adam G

IP-FCM measures physiologic protein-protein interactions modulated by signal transduction and small-molecule drug inhibition.

• DOI: 10.1371/journal.pone.0045722
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Smith SE;Bida AT;Davis TR;Sicotte H;Patterson SE;Gil D;Schrum AG
• 通讯作者: Schrum AG

Toward T cell protein-protein interaction activity relevant to alopecia areata.

与斑秃相关的 T 细胞蛋白质-蛋白质相互作用活性。

• DOI: 10.1038/jidsymp.2013.9
• 发表时间: 2013
• 期刊: The journal of investigative dermatology. Symposium proceedings
• 作者: Neier,StevenC;Smith,StephenEP;Davis,TessaR;Gil,Diana;Schrum,AdamG
• 通讯作者: Schrum,AdamG