Octet Red384

八位组红384

• 批准号: 9272704
• 负责人: Peter Gordon Barr-Gillespie
• 金额: $ 31.79万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-15 至 2018-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9272704
• 关键词: Affinity; Anemia; Binding; Biosensor; Detection; Disease; Funding; HIV; Health Sciences; Institution; Interferometry; Measures; Mental disorders; Methods; Oregon; Productivity; Protein Chemistry; Proteins; Proteomics Shared Resource; Research; Research Personnel; Speed; Universities; hearing impairment; human disease; improved; instrument; protein protein interaction; tool

Project Summary/Abstract We request funds to purchase a ForteBio Octet Red384 instrument to measure protein-protein interactions. To maximize research productivity, the upgraded instrument will be placed in the Oregon Health & Science University Proteomics Shared Resource. The only instruments that measure rates and affinities of protein-protein interactions available at OHSU provide very low throughput, and new instrument is required to increase research productivity at our institution. The new instrument will be used to study the cause and treatment of several diseases including psychiatric disorders, HIV, hearing loss, and anemia, as well as to study methods for treating those diseases. Purified proteins will be analyzed by binding one partner to cheap and dispensable biosensor probes, then using biolayer interferometry to measure binding of a second protein with high sensitivity. The Octet Red384 can efficiently measure many complete sets of interactions per day, each involving 6-8 protein concentrations and producing both kon and koff as well as KD. The introduction of this instrument will fulfill the growing needs of the researchers at the OHSU to incorporate state-of-the-art tools in protein chemistry into their research. The capability afforded by this instrument will speed the progress of discovery and improve the detection and treatment of human disease.

项目摘要/摘要 我们请求资金购买一台ForteBio Octet Red384仪器来测量蛋白质之间的相互作用。至 最大限度地提高研究效率，升级后的仪器将被放置在俄勒冈州健康与科学馆 大学蛋白质组学共享资源。唯一测量蛋白质-蛋白质速率和亲和力的仪器 OHSU提供的交互作用提供的吞吐量非常低，需要新的仪器来增加研究 我们机构的生产力。这种新仪器将被用来研究几种 疾病，包括精神障碍、艾滋病毒、听力损失和贫血，以及研究治疗方法 那些疾病。纯化的蛋白质将通过将一个配对结合到廉价的、不必要的生物传感器上进行分析。 探针，然后使用生物层干涉法以高灵敏度测量第二个蛋白质的结合。八重奏 Red384每天可以有效地测量许多完整的相互作用组，每个组涉及6-8个蛋白质 浓度和产生Kon和Koff以及Kd。这一工具的引入将实现 OHSU的研究人员日益增长的需求，将最先进的蛋白质化学工具纳入他们的 研究。该仪器所提供的能力将加快发现的进度，并改进 人类疾病的检测和治疗。